The role of single nucleotide polymorphisms of NFATC genes in the development of left ventricular hypertrophy in primary arterial hypertensive adolescents

Authors

  • A.V. Tovma Zaporizhzhia State Medical University, Ukraine,
  • A. M. Kamyshnyi Zaporizhzhia State Medical University, Ukraine,
  • A. V. Kamenshchyk Zaporizhzhia State Medical University, Ukraine,
  • O. G. Ivanko Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1237.2017.3.118322

Keywords:

left ventricular hypertrophy, genetic polymorphism, NFATC transcription factors, high blood pressure, adolescents

Abstract

The aim of the study was to determine the possibility of association of the single nucleotide polymorphisms (SNP) of NFATC1 and NFATC4 genes with the development of concentric hypertrophy of the left ventricle (LVH) in adolescents aged 16-18 with newly diagnosed primary hypertension (AH).

Material and methods. The study included 74 adolescents aged 16-18 years (50 boys and 24 girls) who for the first time in the course of preventive medical examination were identified as primary hypertensive carriers. According to the echocardioscopy (Echo-CS) the concentric hypertrophy of the left ventricle was shown in 32 (43%) of adolescents. Other 42 (57%) young people with AH were identified as a control group because their Echo-CS and еlectrocardiography (ECG) data were within the age limits. At the next stage of the study for all the patients the genotyping by a standard PCR method in a real time was performed using samples of total DNA isolated from the whole venous blood.

Results. As shown by the results of genotyping and statistical analysis SNP association with LVH was detected in relation to rs2229309 of the NFATC4 gene and not confirmed for SNP (rs7240256, rs11665469, rs754505) of the NFATC1 gene. Statistically significant differences of incidence (p <0.05) were found for the heterozygous genotype status of this SNP which is almost 2 times more common for adolescents with primary hypertension complicated with early LVH. Predictive significance of the sign "heterozygous genotype CG rs2229309 of the NFATC4 gene" for the development of concentric LVH in adolescents aged 16-18 with primary hypertension was sensitive in 46%, specific in 74% and accurate in 62% of cases. The odds ratio of development of concentric LVH in the adolescents with the detected symptom was 2.43 with the 95% confidence interval of 0.78 to 7.58. At the same moment the predominance of the G allele in these adolescents also was established.

Conclusions. There is an obvious association between the heterozygous condition of CG rs2229309 of NFATC4 gene and early LVH development in adolescents with primary arterial hypertension, diagnosed at the age of 16-18. Also this people are prone to be carriers of the G allele. The genotyping of rs2229309 of the NFATC4 gene can be useful in prognosis of the risk of LVH development in adolescents with AH.

References

Mancia, G., Fagard, R., Narkiewicz, K., Redón, J., Zanchetti, A., Böhm, M., et al. (2013). Guidelines for the Management of Arterial Hypertension: The Task Force for the Management of Arterial Hypertension of the European Society of Hypertension (ESH) and of the European Society of Cardiology (ESC). J Hypertens, 31(7), 1281–357. doi: 10.1097/01.hjh.0000431740.32696.cc

Korenev, N. M., Bogmat, L. F., & Nosova, Ye. M. (2010). Arterial'naya gipertenziya podrostkovogo vozrasta: rasprostranennost', mekhanizmy formirovaniya, podkhody k lecheniyu [Hypertension prevalence of adolescent age: the formative mechanisms, approaches for treatment]. Pediatria, akusherstvo ta hinekolohia, 72(4), 68–69. [in Russian].

Gazieva, Kh. Sh., Mirzaeva, B. M., & Usmanova, U.Sh. (2017). Izuchenie osobennostej techeniya arterial'noj gipertenzii u podrostkov i lic molodogo vozrasta [Study of the features of the course of arterial hypertension in adolescents and young people]. Molodoj uchenyj, 6(140), 144–148. [in Russian].

Kozlova, A. S., Lebed', T. L., Malinovskaya, Y. V., & Melnov, S. B. (2014). Geneticheskie markery serdechno-sosudistoj patologii sportsmenov sporta vysshikh dostizhenij [Genetic markers of cardiovascularpathology in combat sport athletes]. E'kologichekij vestnik, 2(28), 42–49. [in Russian].

Kamenshchyk, А. V., Ivanko, O. G., & Fеdchеnkо, A. V. (2015). Nuklearnyi faktor aktyvovanykh T-klityn (NFATC) yak mozhlyvyi diahnostychnyi i prohnostychnyi marker pry klapannykh vrodzhenykh vadakh sertsia ta hipertrofii miokarda [Nuclear factor of activated T-cells (NFATC) as a possible diagnostic and prognostic marker in congenital valvular diseases and myocardial hypertrophy]. Pathologia, 1, 4–7. [in Ukranian]. doi: https://doi.org/10.14739/2310-1237.2015.1.42815

Ahmetov, I. I., & Fedotovskaya, O. N. (2015). Current Progress in Sports Genomics. Advances in clinical chemistry, 70, 247–314. doi: 10.1016/bs.acc.2015.03.003.

Maidannyk, V. H., Koreniev, M. M., Khaitovych, M. V., & Bohmat, L. F. (2006). Diahnostyka ta klasyfikatsiia pervynnoi arterialnoi hipertenzii u ditei [Diagnosis and classification of primary arterial hypertension in children]. Pediatriia, akusherstvo ta hinekolohiia, 6, 3–10. [in Ukranian].

Teregulov, Yu. E., Teregulova, E. T., Mayanskaya, S. D., & Latipova, Z. K. (2014). Remodelirovanie levogo zheludochka: geometricheskie i e'lektrokardiograficheskie sopostavleniya [Remodeling of the left ventricle: geometrical and electrocardiographic comparisons]. Prakticheskaiya medicina, 3(79), 149–154. [in Russian].

Ivanko, O. G., Tovma, A. V., Kamenshchyk, A. V., & Patsera, M. V. (2017). Indeks Sokolow–Lyon u podrostkov s arteryal'noj gipertenzyej v prognoze koncentricheskoj gipertrofii levogo zheludochka serdca v otdalennyj period nablyudeniya [Prognostic role of Sokolow-Lyon criterion in further development of the left ventricular concentric hypertrophy in adolescents with arterial hypertension]. Zaporozhye medical journal, 1(100), 14–19. [in Russian]. doi: 10.14739/2310-1210.2017.1.91605

Szumilas, M. (2010). Explaining Odds Ratios. J Can Acad Child Adolesc Psychiatry, 19(3), 227–229.

Glanc, S. (1999). Mediko-biologicheskaya statistika [Medical and Biological Statistics]. Moscow: Praktika [in Russian].

How to Cite

1.
Tovma A, Kamyshnyi AM, Kamenshchyk AV, Ivanko OG. The role of single nucleotide polymorphisms of NFATC genes in the development of left ventricular hypertrophy in primary arterial hypertensive adolescents. Pathologia [Internet]. 2017Dec.22 [cited 2024Nov.13];(3). Available from: http://pat.zsmu.edu.ua/article/view/118322

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Section

Original research