Determination of the quantity and topography dynamics of the LCA+-dendritic cells in rat thymus in early postnatal period in norm and after the prenatal administration of the dexamethasone

Authors

  • M. A. Voloshyn Zaporizhzhia State Medical University, Ukraine,
  • Ye. O. Aravitskiy Zaporizhzhia State Medical University, Ukraine,
  • O. G. Kushch Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1237.2017.3.118757

Keywords:

LCA -dendritic cells, dexamethasone, thymus gland

Abstract

Purpose was to study the dynamics of LCA+-dendritic thymus cells in newborn rats in the postnatal period after administration of dexamethasone in the intrauterine period.

Materials and methods. The study was performed on 144 white nonlinear rats on the 1st–3rd, 5th, 9th, 14th, 21st, 30th days after birth. There are 3 groups with 48 rats in each group: 1 – intact group; 2 – experimental group of rats, who received peritoneal injection of 0.05 ml of dexamethasone (in dilution 1:40) on the 18th day of the prenatal period, and a control group of rats receiving 0.05 ml of 0.9 % NaCl according to M. A. Voloshyn. To study the dynamics of dendritic cells in the thymus carrying the α-D mannose receptors, LCA-lectin was used.

Results. It has been established that the maximum content of LCA+-dendritic thymus cells in the intact (3.92 ± 0.65), experimental (3.41 ± 0.49) and control (4.07 ± 0.53) groups occurs on the 1st day after birth. The minimum number of LCA+-dendritic cells was detected on the 5th day in the intact (1.88 ± 0.37) and control (1.73 ± 0.34) groups, and their minimum in the experimental group (2.35 ± 0.48) was determined on the 30th day after birth. In the experimental group from the 5th day to the 9th day a significantly higher number of LCA+-dendritic cells than in the comparison groups was detected.

Conclusions. The introduction of dexamethasone in the prenatal period leads to the significant increase in the LCA+-dendritic cell content in the experimental group from the 5th to the 9th day of life, which indirectly coincides with the increase in the number of peripheral and mitotic dividing lymphocytes in the thymus of rats in the postnatal period.

References

Voloshin, N. A., & Aravickij, E. O. (2017) Osobennosti reaktivnosti subkapsulyarnoj zony timusa posle vnutriutrobnogo gormonal´nogo vozdejstviya [Features of the reactivity of the subcapsular zone of the thymus after intrauterine hormonal action.]. Suchasni aspekty medytsyny ta farmatsii. Proceedings of the Conference, (p. 5–6). Zaporizhzhia. [in Russian].

Voloshyn, M. A., & Hryhorieva, O. A. (2011) Tymus novonarodzhenykh [Thymus of newborn]. Zaporizhzhia. [in Ukrainian].

Voloshin, N. A., & Grigor´eva, E. A. (2005). Lektiny zhivotnogo i rastitel'nogo proiskhozhdeniya: rol' v processakh morfogeneza (obzor literatury i sobstvennykh issledovanij) [Lectins of animal and plant origin: the role in the processes of morphogenesis (review of literature and own research)]. Zhurnal AMN Ukrainy, 11(2), 223–237. [in Russian].

Kushch, O. H., & Voloshyn, M. A. (2010). Metodyka vyvchennia populiatsii γδ -T-limfotsytiv iz vykorystanniam paneli lektyniv [The methodology of the population population γδ-T- lymphocytes in the panel of lectins]. Visnyk morfolohii, 16(1), 76–80. [in Ukrainian].

(2008) Nakaz Ministerstva okhorony zdorov’ia Ukrainy vid 03.11.2008 r. №624 Pro vnesennia zmin do nakazu MOZ Ukrainy vid 15 hrudnia 2003 roku №582 «Pro zatverdzhennia klinichnykh protokoliv z akusherskoi ta hinekolohichnoi dopomohy», nakazu MOZ vid 31.12.2004 roku №676 «Pro zatverdzhennia klinichnykh protokoliv z akusherskoi ta hinekolohichnoi dopomohy» [The order of the Ministry of Health of Ukraine from November, 3, 2008, №624 On Amendments to the Order of the Ministry of Health of Ukraine of December, 15, 2003 No.582 "On Approval of Clinical Protocols on Obstetric and Gynecological Aid", MOH Order of December, 31, 2004 No.676 "On Approval of Clinical Protocols on Obstetric and Gynecological Aid]. [in Ukrainian].

Delamarre, L., & Cohn, L. (2014). Dendritic cell-targeted vaccines. Frontiers in Immunology, 5, 255–260. doi: 10.3389/fimmu.2014.00255

Moleriu, R. D., Zaharie, D., Moatar-Moleriu, L. C., Gruia, A. T., Mic, A. A., & Mic, F. A. (2014). Insights into the mechanisms of thymus involution and regeneration by modeling the glucocorticoid-induced perturbation of thymocytepopulations dynamics. J Theor Biol., 348(2), 84–87 doi: 10.1016/j.jtbi.2014.01.020

Sancho, D., & Reis e Sousa, C. (2012). Signaling by myeloid C-type lectin receptors in immunity and homeostasis. Annu Rev. Immunol. 30, 491–529. doi: 10.1146/annurev-immunol-031210-101352

Hrvačić, B., Šitum, K., Đurić, K., Bošnjak, B., Ferenčić, Ž., Brajša, K., et al. (2015). Relative potencies of three glucocorticoids to induce hypoplasia of the physis and concomitant biochemical alterations in the rat. Drug Chem Toxicol., 38(3), 272–277. doi: 10.3109/01480545.2014.947502

Taves, M. D., Plumb, A. W., Sandkam, B. A., Ma, Ch., Van Der Gugten, J. G., Holmes, D. T. et al. (2012). Steroid profiling reveals widespread local regulation of glucocorticoid levels during mouse development. Endocrinology, 156(2), 511–22. doi: https://doi.org/10.1210/en.2013-1606

Zhang, J. G., Czabotar, P. E., Policheni, A. N., Caminschi, I., Wan, S. S., Kitsoulis, S., et al. (2012). The dendritic cell receptor Clec9A binds damaged cells via exposed actin filaments. Immunity, 36(4), 646–57. doi: 10.1016/j.immuni.2012.03.009.

How to Cite

1.
Voloshyn MA, Aravitskiy YO, Kushch OG. Determination of the quantity and topography dynamics of the LCA+-dendritic cells in rat thymus in early postnatal period in norm and after the prenatal administration of the dexamethasone. Pathologia [Internet]. 2017Dec.22 [cited 2024Nov.23];(3). Available from: http://pat.zsmu.edu.ua/article/view/118757

Issue

Section

Original research