Peculiarities of lymphocytes emigration from newborn thymus

Authors

  • O. A. Grygorieva Zaporizhzhia State Medical University, Ukraine,
  • O. A. Apt Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1237.2017.3.118765

Keywords:

thymus, lymphocytes, lymphatic vessels, mast cells

Abstract

Introduction. Mechanisms of mass lymphocytes’ emigration from thymus are still unclear. The process of lymphocytes migration and mechanisms, which provide it, form one of the important problems of immunomorphology.

Aim of the work: to establish the peculiarities of newborn thymus structure and to reveal pathways and mechanisms which provide lymphocytes’ emigration from thymus.

Materials and methods. Morphological, histological and histochemical analyses of 160 rats’ thymuses from birth up to the 7th day after birth were conducted and discussed. The animals were handled according to the European Commission Directive (86/609/EEC). The profound microscopic analysis of cell contents, lymphatic vessels morphology in morpho-functional areas of rats’ thymuses during the first week after birth in 4 hours interval was carried out. All quantitative results were estimated by the statistic methods.

Results. It is settled that thymus specific density tightly depends on the quantity of degranulated mast cells, absolute quantity of microcirculatory vessels, especially of lymphatic ones. The index of specific density, lymphocyte-epithelial index change wavy during the first week after birth every 12 hours. These changes are followed by the changes of extracellular matrix structure, absolute quantity of small lymphocytes in thymic cortex and lymphocytes’ adhesive properties. The key periods are: from 0 till 2, from 12 till 16, and from 108 till 120 hours after birth. At that time discrete migration of lymphocytes from the thymus occurs through paravasal lymphatic vessels. After temporal edema reduction, lymphatic vessels turn to be empty, their quantity decreases.

Conclusion. Therefore, emigration of lymphocytes from thymus takes place through lymphatic vessels on the top of periodical short-term local edema.

References

Crisa, L., Cirulli, V., Ellisman, M. H., Ishii, J. K., Elices, M. J., & Salomon, D. R. (1996) Cell adhesion and migration are regulated at distinct stages of thymic T cell development: the roles of fibronectin, VLA4, and VLA5. J. Exp. Med., 184(1), 215–28. doi: 10.1084/jem.184.1.215

Jotereau, F., Heuze, F., Salomon-Vie, V., & Gascan, H. (1987) cell kinetics in the fetal mouse thymus: precursor cell input, proliferation, end emigration. J. of Immunology, 138(4), 1026–1030.

Kawakami, N., Nishizawa, F., Sakane, N., Iwao, M., Tsujikawa, K., Ikawa, M., et al. (1999) Roles of integrins and CD44 on the adhesion and migration of fetal liver cells to the fetal thymus. J. of Immunololgy., 163, 3211–3216.

Mori, K., Itoi, M., Tsukamoto, N., Kubo, H., & Amagai, T. (2007) The perivascular space as a path of hematopoietic progenitor cells and mature T cells between blood circulation and the thymic parenchyma. International Immunology, 19(6), 745–753. doi: 10.1093/intimm/dxm041

Weinreich, M. A., & Hogquist, K. A. (2008) Thymic emigration: when and how T cells leave home. The journal of immunology, 181, 2265–2270. doi: https://doi.org/10.4049/jimmunol.181.4.2265

Wilkinson, B., Owen, J J., & Jenkinson, E. J. (1999) Factors regulating stem cell recruitment to the fetal thymus. J. Immunology, 162, 3873–3881.

Voloshyn, M. A (2005) Limfotsyt – faktor morfoheneza [Lymphocyte is a factor of morphogenesis]. Zaporozhye medical journal, 3, 122 [in Ukrainian]

Voloshyn, N. A., Karzow, M. V., Grigoreva.,E. A., Kusch, O. G., Medvedev, A. E., & Popravko, M. I. (2002). Vnutriutrobnoe vvedenie antigena – model' dlya izucheniya processov morphogeneza limphoidnykh organov [Intrauterine antigen injection - a model for studying the processes of morphogenesis of lymphoid organs]. Tavricheskij mediko-biologicheskij vestnik, 5(3), 43–46 [in Russian].

Voloshin, N. A., & Grigor'eva, E. A. (2011) Timus novorozhdennykh [Thymus of newborns]. Zaporizhzhia: ZGMU [in Russian].

Sviridov, A. I. (1966) Anatomicheskij atlas limphaticheskikh kapillyarov [Anatomical atlas of lymphatic capillaries]. Kyiv: Zdorovya [in Russian].

Yarilin, A. A., Pinchuk, V. G., & Grinevich, Yu. A. (1991) Struktura tymusa I differencirovka T-limphocitov [Thymus structure and differentiation of T-lymphocytes]. Kyiv: Nauka [in Russian].

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How to Cite

1.
Grygorieva OA, Apt OA. Peculiarities of lymphocytes emigration from newborn thymus. Pathologia [Internet]. 2017Dec.22 [cited 2024Jun.13];(3). Available from: http://pat.zsmu.edu.ua/article/view/118765

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Original research