Characteristics of the nitric oxide system indicators in the left ventricle myocardium in SHR
DOI:
https://doi.org/10.14739/2310-1237.2018.3.151670Keywords:
NOS isoforms, nitric oxide synthase, ventricular remodeling, left ventricle, heart, essential hypertension, rats, SHRAbstract
The aim was to determine the morpho-functional parameters of the left ventricular myocardium NO system in the rats with essential hypertension (SHR line).
Material and methods. We used a combination of modern highly informative methods, namely: research of NOS isoform profie (nNOS, iNOS eNOS) in the myocardial slices along with an assessment of their synthesis and expression of the corresponding mRNA; NO derived nitrites level determination directly in the myocardium homogenates and concentration of nitrotyrosine in blood plasma of rats.
The results of the performed studies have shown that high blood pressure in the SHR was accompanied by a signifiant increase in the concentrations of all three NOS isoforms in the myocardium and increased expression of
their mRNA. Higher concentration of nitrites by 18.8 % was detected in the SHR group compared with the control animals. The concentration of nitrotyrosine in blood plasma of rats with essential hypertension was also increased by 25 %.
Conclusions. The predominance of IRM to constitutive isoforms of NOS with low IRM content to iNOS was noted in the myocardium of the control group rats while in SHR rats higher IRM values were marked for all NOS isoforms. The formation of hypertension is accompanied by high content of NO end metabolites and the development of systemic nitroso-oxidative stress with the increase of nitrotyrosine concentration.
References
Manukhina, E. B., & Malyshev, I. Yu. (2003). Rol' oksida azota v razvitii i preduprezhdenii disfunkcii e'ndoteliya [The role of nitric oxide in the development and prevention of endothelial dysfunction]. Vestnik Vitebskogo gosudarstvennogo medicinskogo universiteta, 2(2), 5–17. [in Russian].
Ruetten, H., Dimmeler, S., Gehring, D., Ihling, C., & Zeiher, A. (2005) Concentric left ventricular remodeling in endothelial nitric oxide synthase knockout mice by chronic pressure overload. Cardiovascular Research, 66(3), 444–453. doi: 10.1016/j.cardiores.2005.01.021.
Tsutsui, M., Shimokawa, H., Otsuji, Y., & Yanagihara, N. (2010) Pathophysiological relevance of NO signaling in the cardiovascular system: Novel insight from mice lacking all NO synthases. Pharmacology & Therapeutics, 128(3), 499–508. doi: 10.1016/j.pharmthera.2010.08.010.
Shimokawa, H., & Tsutsui, M. (2010) Nitric oxide synthases in the pathogenesis of cardiovascular disease. Pflügers Archiv – European Journal of Physiology, 459(6), 959–967. doi: 10.1007/s00424-010-0796-2.
Antonio, E., Serra, A., Santos, A., Vieira, S., Silva, J., Yoshizaki, A., et al. (2014) Are there gender differences in left ventricular remodeling after myocardial infarction in rats? Revista Brasileira de Cirurgia Cardiovascular, 30(1), 70–76. doi: 10.5935/1678-741.20140093.
Camici, G., Savarese, G., Akhmedov, A., & Lüscher, T. (2015) Molecular mechanism of endothelial and vascular aging: implications for cardiovascular disease. European Heart Journal, 36(48), 3392–3403. doi: 10.1093/eurheartj/ehv587.
Fedotova, M. I., Kovalov, M. M., Zhulinskyi, V. O., & Kadzharian Ye. V. (2017) Оsoblyvosti ekspresii izoform sintazy oksydu azotu u miokardi livoho shlunochka schuriv pry arterialnii hipertenzii riznoho henezu. Aktualni problemy suchasnoi medytsyny [Features of expression of isoforms of nitric oxide synthase in myocardium of the left ventricle of rats with arterial hypertension of different genesis]. Visnyk Ukrainskoi medychnoi stomatolohichnoi akademii, 17, 4(60), 91–95. [in Ukrainian].
Zherebiatiev, A., & Kamyshny, A. (2016) Expression Levels of Proinflammatory Cytokines and NLRP3 Inflammasome in an Experimental Model of Oxazolone-induced Colitis. Iran J Allergy Asthma Immunol, 15(1), 39–45.
Gorbunov, N. V. (1995) Opredelenie stabil'nykh metabolitov oksida azota po Grissu v biologicheskom materiale [Determination of stable nitric oxide metabolites by Griss in biological material]. Byulleten' e'ksperimental'noj biologii i mediciny, 7, 40–48. [in Russian].
Zajcev, V. M., Liflyandskij, V. G., & Marinkin, V. I. (2003) Prikladnaya medicinskaya statistika [Applied Medical Statistics]. [in Russian].
Shabeeh, H., Khan, S., Jiang, B., Brett, S., Melikian, N., Casadei, B., et al. (2017) Blood Pressure in Healthy Humans Is Regulated by Neuronal NO Synthase Novelty and Significance. Hypertension, 69(5), 970–976. doi: 10.1161/HYPERTENSIONAHA.116.08792.
Jang, J., Chun, J., Godo, S., Wu, G., Shimokawa, H., Jin, C., et al. (2015) ROS and endothelial nitric oxide synthase (eNOS)-dependent trafficking of angiotensin II type 2 receptor begets neuronal NOS in cardiac myocytes. Basic Research in Cardiology, 110(3), 1–23. doi: 10.1007/s00395-015-0477-6.
Dokken, B., Gaballa, M., Hilwig, R., Berg, R., & Kern, K. (2015) Inhibition of Nitric Oxide Synthases, But Not Inducible Nitric Oxide Synthase, Selectively Worsens Left Ventricular Function After Successful Resuscitation From Cardiac Arrest in Swine. Academic Emergency Medicine, 22(2), 197–203. doi: 10.1111/acem.12575.
Yuyun, M., Ng, L., & Ng, G. (2018) Endothelial dysfunction, endothelial nitric oxide bioavailability, tetrahydrobiopterin, and 5-methyltetrahydrofolate in cardiovascular disease. Where are we with therapy? Microvascular Research, 119, 7–12. doi: 10.1016/j.mvr.2018.03.012.
Viaro, F., Nobre, F., & Roberto, P. (2000) Expression of nitric oxide synthases in the pathophysiology of cardiovascular diseases. Arquivos Brasileiros de Cardiologia, 74(4), 380–393. http://dx.doi.org/10.1590/S0066-782X2000000400009
Kravchenko, N. A., & Yarmysh, N. V. (2008) Regulyaciya e'kspressii e'ndotelial'noj NO-sintazy i disfunkciya sosudistogo e'ndoteliya pri serdechno-sosudistoj patologii [Regulation of expression of endothelial NO synthase and dysfunction of vascular endothelium in cardiovascular pathology]. Citologiya i genetika, 4, 69–79. [in Russian].
Champion, H. C., Georgakopoulos, D., Takimoto, E., Isoda, T., Wang, Y., & Kass, D. A. (2004) Modulation of in vivo cardiac function by myocyte-specific nitric oxide synthase-3. Circulation Research, 94(5), 657–63. doi: 10.1161/01.RES.0000119323.79644.20.
Buchwalow, I., Schulze, W., Karczewski, P., Kostic, M. M., Wallukat, G., Morwinski, R., et al. (2001) Inducible nitric oxide synthase in the myocard. Molecular and Cellular Biochemistry, 217(1–2), 73–82.
Drummond, G., Cai, H., Davis, M., Ramasamy, S., & Harrison, D. (2000) Transcriptional and Posttranscriptional Regulation of Endothelial Nitric Oxide Synthase Expression by Hydrogen Peroxide. Circulation Research, 86(3), 347–354. doi: 10.1161/01.RES.86.3.347.
Álvarez, M., Caldiz, C., Fantinelli, J., Garciarena, C., Console, G., Chiappe de Cingolani, G., et al. (2008) Is Cardiac Hypertrophy in Spontaneously Hypertensive Rats the Cause or the Consequence of Oxidative Stress? Hypertension Research, 31(7), 1465–1476. doi: 10.1291/hypres.31.1465.
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
