Morphometric and densitometric characteristics of the brainstem locus coeruleus neurons nuclei in rats with experimental arterial hypertension

Authors

  • O. V. Hancheva Zaporizhzhia State Medical University, Ukraine,
  • M. V. Danukalo Zaporizhzhia State Medical University, Ukraine,
  • O. V. Melnikova Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1237.2019.1.166145

Keywords:

densitometry, cariometry, brainstem, locus coeruleus, arterial hypertension, rats

Abstract

The aim of our study was to establish the morphodensitometric features of locus coeruleus (LC) neurons nuclei in the brain stem of rats with arterial hypertension of various origin (essential – SHR and endocrine-salt hypertension (ESH)).

Matherials and methods: the study was carried out on 30 mature male rats: 10 control Wistar rats, 10 Wistar rats with modeled endocrine-salt AH (ESH) and 10 spontaneously hypertensive rats (SHR) with genetically determined hypertension. The histochemical method of staining with gallocyanine-chrome alum by Einarson was used for the evaluation of morphodensitometric characteristics of the neurons nuclei: the nucleus area, the content and concentration of nucleic acids (NA) in the nucleus.

Results: it was found that the highest indices were observed in rats of the SHR line while in rats with ESH the content, RNA concentration and nucleus area decreased. In SHR rats with essential hypertension high synthetic activity was observed in the LC neurons with an increase of the content and concentration of NA.

Conclusions. Morphometric and densitometric characteristics of the of the brain stem LC in AH depend on the etiological factor and pathogenesis of hypertension. In essential AH of SHR there is a high synthetic activity in the brainstem LC neurons nuclei along with an increase in the content and concentration of NA. In symptomatic AH in rats with ESH, on the contrary, the structure activity is decreased and characterized by the reduction in nucleus size and NA content in it.

References

Swischenko, E., Bahrii, A. E., Yena, L. M., Kovalenko, V. M., Koval, S. M., Mellina, I. M., et al. (2012). Arterialna hipertenziia. Onovlena ta adaptovana klinichna nastanova, zasnovana na dokazakh [Arterial hypertension. An updated and adapted clinical guideline based on evidence]. Novosti mediciny i farmacii, 12(422), 24–31. [in Ukrainian].

Perez, H., Ruiz, S., Nunez, H., White, A., Gotteland, M., & Hernandez, A. (2006). Paraventricular–coerulear interactions: role in hypertension induced by prenatal undernutrition in the rat. European Journal of Neuroscience, 24(4), 1209–1219. doi: 10.1111/j.1460-9568.2006.04997.x

Chertok, V. M., & Kotsyuba, A. E. (2015). Norepinephrinergic and nitroxidergic neurons of vasomotor nuclei in hypertensive rats. Bulletin of experimental biology and medicine, 158(5), 695–699. doi: 10.1007/s10517-015-2838-4

Kotsiuba, A. E., Chertok, V. M., & Babich, E. V. (2010). Nitroksidergicheskie nejrony bul'barnogo vazomotornogo centra pri arterial'noj gipertenzii [Nitricoxideergic neurons of the human bulbar vasomotor center in arterial hypertension]. Zhurnal nevrologii i psikhiatrii im. C.C. Korsakova, 110(2), 61–65. [in Russian].

Feinstein, D. L., Kalinin, S., & Braun, D. (2016). Causes, consequences, and cures for neuroinflammation mediated via the locus coeruleus: noradrenergic signaling system. Journal of neurochemistry, 139(2), 154–178. doi: 10.1111/jnc.13447

Aston-Jones, G., & Waterhouse, B. (2016). Locus coeruleus: from global projection system to adaptive regulation of behavior. Brain research, 1645, 75–8. doi: 10.1016/j.brainres.2016.03.001

Samuels, E. R., & Szabadi, E. (2008). Functional neuroanatomy of the noradrenergic locus coeruleus: its roles in the regulation of arousal and autonomic function part I: principles of functional organisation. Current neuropharmacology, 6(3), 235–53. doi: 10.2174/157015908785777229

O’Donnell, J., Zeppenfeld, D., McConnell, E., Pena, S., & Nedergaard, M. (2012). Norepinephrine: a neuromodulator that boosts the function of multiple cell types to optimize CNS performance. Neurochemical research, 37(11), 2496–512. doi: 10.1007/s11064-012-0818-x

Wood, C. S., Valentino, R. J., & Wood, S. K. (2017). Individual differences in the locus coeruleus-norepinephrine system: Relevance to stress-induced cardiovascular vulnerability. Physiology & behavior, 172, 40–48. doi: 10.1016/j.physbeh.2016.07.008

Wang, X., Piñol, R. A., Byrne, P., & Mendelowitz, D. (2014). Optogenetic stimulation of locus ceruleus neurons augments inhibitory transmission to parasympathetic cardiac vagal neurons via activation of brainstem α1 and β1 receptors. Journal of Neuroscience, 34(18), 6182–9. doi: 10.1523/JNEUROSCI.5093-13.2014

Tverskoy, A. V., Dolzhikov, A. A., Bobintsev, I. I., Kryukov, A. A., & Belykh, A. E. (2014). Morfologicheskiye izmeneniya neyronov oblastey CA1 i CA3 gippokampa krys pri hronicheskom immobilizacyonnom stresse (morfometricheskoye issledovanie) [Morphological changes of CA1 and CA3 hippocampal regions in rats under chronic immobilization stress (morphometric study)]. Kurskij nauchno-prakticheskij vestnik “Chelovek i ego zdorov'e”, 3, 37–41. [in Russian].

Kadzharyan, E. V. (2013) Osobennosti funcional`nogo sostoyaniya beta-e'ndorfin synteziruyuschikh nejronov paraventricularnogo yadra hipotalamusa krys v norme i pri e'ksperimental`nom sakharnom diabete [The peculiarities of the functional state of beta-endorphinsynthesizing neurons of the paraventricular nucleus of rats’ hypothalamus in normal conditions and with experimental diabetes mellitus]. Perspektyvy medytsyny ta biolohii, 5(1), 78–82. [in Russian].

Camacho, P., Fan, H., Liu, Z., & He, J. Q. (2016). Small mammalian animal models of heart disease. American journal of cardiovascular disease, 6(3), 70–80.

Kolesnyk, Yu. M., Hancheva, O. V., Abramov, A. V., Ivanenko, T. V., Tischenko, S. V., Kuzo, N. (patentee) (2015). Patent 102234 Ukraina, MPK G09V 23/28. Sposib modeliuvannia symptomatychnoi arterialnoi hipertezii u dribnykh hryzuniv [Patent 102234 Ukraine, IPC G09V 23/28. Method of simulation of symptomatic arterial hypertension in small rodents]. Bulleten, 20. [in Ukrainian].

Paxinos, G., & Watson, C. H. (1986). Atlas of the rat brain in stereotaxic coordinates. Academic, New York.

Pearse, A. G. E. (1962). Gistokhimiya. Teoreticheskaya i prikladnaya [Histochemistry, Theoretical and Applied]. Moscow: Izdatel`stvo inostrannoy literatyri. [in Russian].

Gajdyshev, I. P. (2015). Modelirovanie stokhasticheskikh i determinirovannykh sistem: rukovodtvo pol`zovatelya programmy AtteStat [Modeling Stochastic and Deterministic Systems: AtteStat Software User Guide]. Kurgan. [in Russian].

Zajcev, V. M., Liflyandskij, V. G., & Marinkin, V. I. (2006). Prikladnaya medicinskaya statistika [Applied medical statistics]. Saint Petersburg: Foliant. [in Russian].

Kourtesis, I., Kasparov, S., Verkade, P., & Teschemacher, A. G. (2015). Ultrastructural correlates of enhanced norepinephrine and neuropeptide Y cotransmission in the spontaneously hypertensive rat brain. ASN Neuro, 7(5), pii: 1759091415610115. doi: 10.1177/1759091415610115

Bliss, E. L., & Zwanziger, J. (1966). Brain amines and emotional stress. Journal of psychiatric research, 4(3), 189–198.

Uzbekov, M. G. (2005). Nejrokhimicheskie aspekty vzaimosvyazi monoaninergicheskikh i gormonal`nykh sistem v patogeneze trevozhnoj depressii [Neurochemical aspects of the relationship between monoaminergic and hormonal systems in the pathogenesis of anxious depression]. Social`naya i klinicheskaya psikhiatriya, 15(2), 108–110. [in Russian].

Svensson, T. H., & Thoren, P. (1979). Brain noradrenergic neurons in the locus coeruleus: inhibition by blood volume load through vagal afferents. Brain Research, 172(1), 174–178. https://doi.org/10.1016/0006-8993(79)90908-9

Downloads

How to Cite

1.
Hancheva OV, Danukalo MV, Melnikova OV. Morphometric and densitometric characteristics of the brainstem locus coeruleus neurons nuclei in rats with experimental arterial hypertension. Pathologia [Internet]. 2019May13 [cited 2024Nov.13];(1). Available from: http://pat.zsmu.edu.ua/article/view/166145

Issue

Section

Original research