Results of experimental modeling of postoperative peritoneal adhesions
DOI:
https://doi.org/10.14739/2310-1237.2019.1.166220Keywords:
animal experimentation, tissue adhesionsAbstract
Objective: Optimization of postoperative intraabdominal adhesions modeling.
Material and methods. The objects of the study were 17 mature rats of the Wistar line of both sexes weighing 200–250 g. Experimental modeling of postoperative intraabdominal adhesions was carried out under aseptic conditions (the 1st series, 11 animals) and in peritonitis conditions (the 2nd series, 6 animals) .
Results. We have used the Matoba score scale for the quantitative assessment of the adhesion process, as well as to analyze the models with the involvement of the cecum: the average score of the adhesion process in the 1st group was 4.90 (3.00; 6.00), in the peritonitis conditions – 4.83 (2.00; 7.00). Formation of the intraperitoneal adhesion process was achieved in all experimental animals (100 %). Formation of adhesions between the cecum and the abdominal wall was noted in 66.7 % of cases.
Conclusions. For the experimental reproduction of intraabdominal adhesions in aseptic conditions, it is advisable to use a method of simulation by abrasions of the serous cover of cecum with a cervical brush along the intestine till the appearance of spot hemorrhages in the form of “blood dew” and damage to the parietal peritoneum in the lower quadrants, which provides the development of adhesive intraabdominal process in all experimental animals. For experimental reproduction of intraabdominal adhesions in peritonitis it is expedient for 20 hours prior to the above-described method of modeling to complete an intraperitoneal injection of 2 ml of fecal suspension into a physiological solution of sodium chloride, which provides the development of an adhesive intra-abdominal process under conditions of fibrinous purulent peritonitis in all experimental animals. The obtained data allowed to consider the developed models to be adequate for further experimental research with the aim of studying intraabdominal prophylaxis of adhesion formation under conditions of infection and in aseptic conditions.
References
Ashurmetov, R. I., & Rizakhodzhaev, A. A. (2012). Modelirovanie spaechnoj bolezni [Modeling of adhesive disease]. Voprosy mediciny: teoriya i praktika. Proceedings of the International Scientific Practical Conference. (P. 107–110). Novosibirsk. [in Russian].
Lipatov, V. A. (2004). Obosnovanie primeneniya gelya metilcellyulozy dlya profilaktiki posleoperacionnogo spaechnogo processa bryushnoj polosti (Avtoref. dis …kand. med. nauk). [Substantiation of the use of methylcellulose gel for the prevention of postoperative adhesions of the abdominal cavity]. (Extended abstract of candidate’s thesis). Kursk. [in Russian].
Sopuev, A. A., Mamatov, N. N., Ovcharenko, K. Ye., Umurzakov, O. A., Ibragimov, U. K. (2011). Ocenka e'ffektivnosti razlichnykh variantov modelirovaniya spaechnogo processa bryushnoj polosti [Evaluation of the effectiveness of various options for modeling the adhesion process of the abdominal cavity]. Aktual'nye problemy gumanitarnykh i yestestvennykh nauk, 3, 327–332. [in Russian].
Zeng, L., Yan, S., Li, W. L., Yang, L., Yue, Y. Z., Jiang, C. X., et al. (2015). Assessment of postoperative adhesion formation in a rat cecum model using different techniques. Pakistan journal of pharmaceutical sciences, 28, 1049–54.
Poehnert, D., Abbas, M., Kreipe, H., Klempnauer, J., & Winny, M. (2015). High Reproducibility of Adhesion Formation in Rat with Meso-Stitch Approximation of Injured Cecum and Abdominal Wall. International Journal of Medical Sciences, 12(1), 1–6. doi: 10.7150/ijms.8870
Wallwiener, M., Brucker, S., Hierlemann, H., Brochhausen, C., Solomayer, E., & Wallwiener, C. (2006). Innovative barriers for peritoneal adhesion prevention: liquid or solid? A rat uterine horn model. Fertility And Sterility, 86(4), 1266–1276. doi: 10.1016/j.fertnstert.2006.05.023
Matoba, M., Hashimoto, A., Tanzawa, A., Orikasa, T., Ikeda, J., Iwame, Y., et al. (2015). Prevention of Polyglycolic Acid-Induced Peritoneal Adhesions Using Alginate in a Rat Model. Biomed Research International, 2015, 403413. doi: 10.1155/2015/403413
Kraemer, B., Wallwiener, C., Rajab, T., Brochhausen, C., Wallwiener, M., & Rothmund, R. (2014). Standardised Models for Inducing Experimental Peritoneal Adhesions in Female Rats. Biomed Research International, 2014, 435056. doi: 10.1155/2014/435056
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
