The combined effect of intermittent normobaric hypoxia and melatonin on the morphological changes of the spontaneously hypertensive rats pancreas

Authors

  • R. V. Yanko Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv,
  • M. I. Levashov Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv,
  • I. H. Litovka Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv,
  • S. L. Safonov Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv,

DOI:

https://doi.org/10.14739/2310-1237.2019.2.177123

Keywords:

pancreas, hypoxia, melatonin, hypertension

Abstract

The aim of the study was to investigate the morphological changes in the pancreas of spontaneously hypertensive animals after the combined effect of intermittent normobaric hypoxia (INH) and melatonin.

Materials and methods. The study was carried out in spring on 24 spontaneously hypertensive male rats (SHR line). The experimental animals were daily given a hypoxic gas mixture (12 % oxygen in nitrogen) in an intermittent mode: 15 min deoxygenation/15 min reoxygenation for 2 hours. The same rats were daily administered orally with exogenous melatonin at 10.00 at a dose of 5 mg/kg. The duration of the experiment was 28 days. Histological preparations were prepared according to a standard procedure. Morphometry of the digital images of preparations was performed using the computer program ImageJ.

Results. Based on changes in morphometric parameters (reduction in the size of acini, exocrinocytes, height of the epithelium), it can be assumed that the combined effect of INH and melatonin reduces the activity of the exocrine function of the pancreas. In the endocrine part of the pancreas of experimental animals morphological signs of its activation were noted: the linear dimensions of the Langerhans islets, the number and the density of their endocrine cells increased. Experimental animals also showed a decrease in the width of interlobular and interacinar interlayers of connective tissue, which can be considered as a manifestation of the mechanism of structural adaptation that facilitates the transport of oxygen and nutrients to the parenchymal elements of the pancreas.

Conclusions. The combined effect of intermittent normobaric hypoxia and melatonin leads to the appearance of morphological signs of a decrease in the activity of the exocrine part of the pancreas in hypertensive rats. At the same time, the activity of the endocrine gland function in these animals increased.

References

Cruickshank, A. H., & Benbow E. W. (1995). Pathology of the pancreas. Berlin, Heidelberg, NewYork, Tokyo: Springer-Verlag.

Berezovskij, V. A. (2012). Prirodnaya i instrumental'naya oroterapiya [Natural and instrumental orotherapy]. Doneck. [in Russian].

Możdżan, M., Możdżan, M., Chałubiński, M., Wojdan, K. & Broncel, M., (2014). The effect of melatonin on circadian blood pressure in patients with type 2 diabetes and essential hypertension. Arch Med Sci., 10(4), 669–75. doi: 10.5114/aoms.2014.44858

Morishima, T., Hasegawa, Y., Sasaki, H., Kurihara, T., Hamaoka, T., & Goto K. (2015). Effects of different periods of hypoxic training on glucose metabolism and insulin sensitivity. Clin Physiol Funct Imaging, 35(2), 104-9. doi: 10.1111/cpf.12133

Fraker, C. A., Ricordi, C., Inverardi, L., & Domínguez-Bendala, J. (2009). Oxygen: a master regulator of pancreatic development? Biol Cell., 101(8), 431–40. doi: 10.1042/BC20080178

Zhuravleva, S. A. (2013). Gistologiya. Praktikum [Histology. Workshop]. Minsk: Vyshejshaya shkola. [in Russian].

Yanko, R. V., Plotnikova, L. N., & Chaka, E. G. (2017). Vliyanie preryvistoj normobaricheskoj gipoksii na morfofunkcional'noe sostoyanie podzheludochnoj zhelezy spontanno-gipertenzivnykh krys [Effect of intermittent normobaric hypoxia on morphofunctional state of pancreas in spontaneously hypertensive rats]. Aktual'ni problemy suchasnoi medytsyny: Visnyk Ukrainskoi medychnoi stomatolohichnoi akademii, 17, 4(60), 1, 68–71. [in Russian].

Yanko, R., Berezovsky, V., Levashov, M., Plotnikova, L., & Chaka, O. (2016). Reaktsiia pidshlunkovoi zalozy spontanno-hipertenzyvnykh shhuriv na diiu ekzohennoho melatoninu u vesnianyi ta osinnii periody roku [Pancreatic response of spontaneously hypertensive rats to the exogenous administration of melatonin in the spring and autumn]. Visnyk Dnipropetrovskoho universytetu. Biolohiia, medytsyna, 7(2), 90–95. [in Ukrainian]. doi: 10.15421/021616

Ota, H., Itaya-Hironaka, A., Yamauchi, A., Sakuramoto-Tsuchida, S., Miyaoka, T., Fujimura, T., et al. (2013). Pancreatic β cell proliferation by intermittent hypoxia via up-regulation of Reg family genes and HGF gene. Life Sci., 93(18–19), 664–72. doi: 10.1016/j.lfs.2013.09.001

Kolesnyk, Y. M., Abramov, A. V., Ivanenko, T. V., & Zhulinskyi, V. O. (2014). Osoblyvosti ekspresii bilkiv BCL-2, P53 ta proliferatyvnoi aktyvnosti v pankreatychnykh ostrivtsiakh pid vplyvom pereryvchastoi hipoksii za umov eksperymentu [The features of expression of BCL-2 and P53 proteins and proliferative activity in pancreatic islets under the influence of intermittent hypoxia in experimental conditions]. Visnyk morfolohii, 20(2), 366–69. [in Ukrainian].

Kushnir, A. Yu., & Davydenko, I. S., (2009). Vplyv melatoninu na stan ostrivtsiv Langergansa pidshlunkovoi zalozy v aloksandiabetychnyh shhuriv [Influence of melatonin on condition of the langergans isles of the pancreas in alloksandiabetic rats]. Svit medytsyny ta biolohii, 4, 31–35. [in Ukrainian].

Lyssenko, V., Nagorny, C. L., Erdos, M. R., Wierup, N., Jonsson, A., Spégel, P., et al. (2009). Common variant in MTNR1B associated with increased risk of type 2 diabetes and impaired early insulin secretion. Nat Genet., 41(1), 82–8. doi: 10.1038/ng.288

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1.
Yanko RV, Levashov MI, Litovka IH, Safonov SL. The combined effect of intermittent normobaric hypoxia and melatonin on the morphological changes of the spontaneously hypertensive rats pancreas. Pathologia [Internet]. 2019Sep.2 [cited 2024Apr.19];(2). Available from: http://pat.zsmu.edu.ua/article/view/177123

Issue

No. 2 (2019)

Section

Original research

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