Activity of matrix metalloproteinases and their tissue inhibitors in serum in chronic granulating periodontitis

Authors

  • D. I. Haidash State Institution “Lugansk State Medical University”, Rubizhne, Ukraine,
  • I. S. Haidash State Institution “Lugansk State Medical University”, Rubizhne, Ukraine,
  • O. О. Bondar State Institution "Lugansk State Medical University", s. Rubizhne, Ukraine,
  • Yu. О. Yevtushenko State Institution "Lugansk State Medical University", Rubizhne, Ukraine,
  • O. I. Haidash State Institution "Lugansk State Medical University", Rubizhne, Ukraine,

DOI:

https://doi.org/10.14739/2310-1237.2019.3.188896

Keywords:

chronic granulating periodontitis, matrix metalloproteinases, tissue inhibitors of matrix metalloproteinases

Abstract

 

Aim. The aim of the work is to study the activity of matrix metalloproteinases and their tissue inhibitors in chronic granular periodontitis.

Materials and methods. We examined serum of 15 patients with chronic granulating periodontitis and 15 healthy male blood donors aged 32 to 37 years. Determination of MMP-8, MMP-9 and TIMP-1 activity was performed by solid-phase enzyme immunoassay using test systems (R&D Diagnostics Inc., USA) on the automatic immunoenzyme complex “GBG Star Fax 2100” produced by “Awareness Technology Inc.” (USA). Statistical evaluation of the results was carried out using the generated database in the program Statistica v. 10.0 (StatSoft. Inc., USA), № STA999K347156-W.

Results. It was established that in the phase of exacerbation of chronic granulating periodontitis in the blood serum of patients there was an increase in concentrations of MMP-8 and MMP-9, which exceeded the content of TIMP-1, which was accompanied by a decrease in the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1, compared with those for healthy individuals. In the phase of early convalescence, 3–5 days after treatment of patients with exacerbation of chronic granulating periodontitis, concentrations of MMP-8 and MMP-9 in the blood serum of the examined patients decreased more dynamically than the concentration of TIMP-1, that was accompanied by an increase in the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1, at the same time the full normalization of MMP-8, MMP-9, TIMP-1 and the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1 did not happen. 

Conclusions. Chronic granulating periodontitis is accompanied by changes in the blood serum of patients with MMP-8, MMP-9 and TIMP-1, which depend on the phase of the pathological process. The highest concentrations of MMP-8, MMP-9 and TIMP-1 are recorded in the acute phase of the disease. In the phase of early convalescence, 3–5 days after the remediation of the focus of inflammation, the concentrations of MMP-8, MMP-9 and TIMP-1 in serum are decreased, while the dynamics of decreasing of the content of MMP-8 and MMP-9 prevails over that for TIMP-1. The coefficients MMP-8/TIMP-1 and MMP-9/TIMP-1, which characterize the balance in the system of MMP/TIMP, allow assessing the state of the system in the dynamics of the disease.

 

References

Borysenko, A. V., Antonenko, M. Yu., Lynovytska, L. V., Sidelnikova, L. F., Nesyn, O. F., Dikova, I. H., … Skyba, I. M. (2017). Stomatolohichni zakhvoriuvannia: terapevtychna stomatolohiia [Dental treatment: therapeutic dentistry]. Kyiv: Medytsyna. [in Ukrainian].

Nazir, M. A. (2017). Prevalence of periodontal disease, its association with systemic diseases and prevention. International Journal of Health Sciences-Ijhs, 11(2), 72-80.

Righolt, A. J., Jevdjevic, M., Marcenes, W., & Listl, S. (2018). Global-, Regional-, and Country-Level Economic Impacts of Dental Diseases in 2015. Journal of Dental Research, 97(5), 501-507. https://doi.org/10.1177/0022034517750572

Tonetti, M. S., Jepsen, S., Jin, L. J., & Otomo-Corgel, J. (2017). Impact of the global burden of periodontal diseases on health, nutrition and wellbeing of mankind: A call for global action. Journal of Clinical Periodontology, 44(5), 456-462. https://doi.org/10.1111/jcpe.12732

Ziebolz, D., Schmalz, G., Kauffels, A., Widmer, F., Widmer, K., Slotta, J. E., . . . Kollmar, O. (2017). Periodontal pathogenic bacteria and aMMP-8 findings depending on periodontal conditions of patients before and after liver transplantation. Clinical Oral Investigations, 21(3), 745-752. https://doi.org/10.1007/s00784-016-1821-4

Kirchner, A., Jager, J., Krohn-Grimberghe, B., Patschan, S., Kottmann, T., Schmalz, G., . . . Ziebolz, D. (2017). Active matrix metalloproteinase-8 and periodontal bacteria depending on periodontal status in patients with rheumatoid arthritis. Journal of Periodontal Research, 52(4), 745-754. https://doi.org/10.1111/jre.12443

de Morais, E. F., Pinheiro, J. C., Leite, R. B., Santos, P. P. A., Barboza, C. A. G., & Freitas, R. A. (2018). Matrix metalloproteinase-8 levels in periodontal disease patients: A systematic review. Journal of Periodontal Research, 53(2), 156-163. https://doi.org/10.1111/jre.12495

Zhang, L., Li, X., Yan, H., & Huang, L. (2018). Salivary matrix metalloproteinase (MMP)-8 as a biomarker for periodontitis A PRISMA-compliant systematic review and meta-analysis. Medicine, 97(3). https://doi.org/10.1097/md.0000000000009642

Franco, C., Patricia, H. R., Timo, S., Claudia, B., & Marcela, H. (2017). Matrix Metalloproteinases as Regulators of Periodontal Inflammation. International Journal of Molecular Sciences, 18(2). https://doi.org/10.3390/ijms18020440

Jasberg, H., Tervahartiala, T., Sorsa, T., Soderling, E., & Haukioja, A. (2018). Probiotic intervention influences the salivary levels of Matrix Metalloproteinase (MMP)-9 and Tissue Inhibitor of metalloproteinases (TIMP)-1 in healthy adults. Archives of Oral Biology, 85, 58-63. https://doi.org/10.1016/j.archoralbio.2017.10.003

Bastos, M. F., Tucci, M. A., de Siqueira, A., de Faveri, M., Figueiredo, L. C., Vallim, P. C., & Duarte, P. M. (2017). Diabetes may affect the expression of matrix metalloproteinases and their inhibitors more than smoking in chronic periodontitis. Journal of Periodontal Research, 52(2), 292-299. https://doi.org/10.1111/jre.12394

Alfakry, H., Malle, E., Koyani, C. N., Pussinen, P. J., & Sorsa, T. (2016). Neutrophil proteolytic activation cascades: a possible mechanistic link between chronic periodontitis and coronary heart disease. Innate Immunity, 22(1), 85-99. https://doi.org/10.1177/1753425915617521

Koymans, K. J., Bisschop, A., Vughs, M. M., van Kessel, K. P. M., de Haas, C. J. C., & van Strijp, J. A. G. (2016). Staphylococcal Superantigen-Like Protein 1 and 5 (SSL1 & SSL5) Limit Neutrophil Chemotaxis and Migration through MMP-Inhibition. International Journal of Molecular Sciences, 17(7). https://doi.org/10.3390/ijms17071072

Ayravainen, L., Heikkinen, A. M., Kuuliala, A., Ahola, K., Koivuniemi, R., Moilanen, E., . . . Sorsa, T. (2018). Anti-rheumatic medication and salivary MMP-8, a biomarker for periodontal disease. Oral Diseases, 24(8), 1562-1571. https://doi.org/10.1111/odi.12930

Fernández, A., Cárdenas, A. M., Astorga, J., Veloso, P., Alvarado, A., Merino, P., . . . Hernández, M. (2019). Expression of toll-like receptors 2 and 4 and its association with matrix metalloproteinases in symptomatic and asymptomatic apical periodontitis. Clinical Oral Investigations, 23(12), 4205-4212. https://doi.org/10.1007/s00784-019-02861-9

Gursoy, U. K., Kononen, E., Tervahartiala, T., Gursoy, M., Pitkanen, J., Torvi, P., . . . Sorsa, T. (2018). Molecular forms and fragments of salivary MMP-8 in relation to periodontitis. Journal of Clinical Periodontology, 45(12), 1421-1428. https://doi.org/10.1111/jcpe.13024

Li, X. T., Lu, J. X., Teng, W., Zhao, C. J., & Ye, X. L. (2018). Quantitative Evaluation of MMP-9 and TIMP-1 Promoter Methylation in Chronic Periodontitis. DNA and Cell Biology, 37(3), 168-173. https://doi.org/10.1089/dna.2017.3948

Gao, Y., Hao, C. D. (2018). Expression of miR-146a in saliva of chronic periodontitis patients and its influence on gingival crevicular inflammation and MMP-8/TIMP-1 levels. Shanghai Kou Qiang Yi Xue. 27(3), 309-312.

Wang, L., Feng, K., & Sun, L. (2014). The influences of non-surgical periodontal treatment on gingival crevicular fluid levels of MMP-8 and tissue inhibitor of TIMP-1. Shanghai Kou Qiang Yi Xue, 23(2), 229-232.

How to Cite

1.
Haidash DI, Haidash IS, Bondar OО, Yevtushenko YО, Haidash OI. Activity of matrix metalloproteinases and their tissue inhibitors in serum in chronic granulating periodontitis. Pathologia [Internet]. 2019Dec.23 [cited 2024Dec.23];(3). Available from: http://pat.zsmu.edu.ua/article/view/188896

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Section

Original research