Activity of matrix metalloproteinases and their tissue inhibitors in serum in chronic granulating periodontitis
DOI:
https://doi.org/10.14739/2310-1237.2019.3.188896Keywords:
chronic granulating periodontitis, matrix metalloproteinases, tissue inhibitors of matrix metalloproteinasesAbstract
Aim. The aim of the work is to study the activity of matrix metalloproteinases and their tissue inhibitors in chronic granular periodontitis.
Materials and methods. We examined serum of 15 patients with chronic granulating periodontitis and 15 healthy male blood donors aged 32 to 37 years. Determination of MMP-8, MMP-9 and TIMP-1 activity was performed by solid-phase enzyme immunoassay using test systems (R&D Diagnostics Inc., USA) on the automatic immunoenzyme complex “GBG Star Fax 2100” produced by “Awareness Technology Inc.” (USA). Statistical evaluation of the results was carried out using the generated database in the program Statistica v. 10.0 (StatSoft. Inc., USA), № STA999K347156-W.
Results. It was established that in the phase of exacerbation of chronic granulating periodontitis in the blood serum of patients there was an increase in concentrations of MMP-8 and MMP-9, which exceeded the content of TIMP-1, which was accompanied by a decrease in the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1, compared with those for healthy individuals. In the phase of early convalescence, 3–5 days after treatment of patients with exacerbation of chronic granulating periodontitis, concentrations of MMP-8 and MMP-9 in the blood serum of the examined patients decreased more dynamically than the concentration of TIMP-1, that was accompanied by an increase in the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1, at the same time the full normalization of MMP-8, MMP-9, TIMP-1 and the ratio of MMP-8/TIMP-1 and MMP-9/TIMP-1 did not happen.
Conclusions. Chronic granulating periodontitis is accompanied by changes in the blood serum of patients with MMP-8, MMP-9 and TIMP-1, which depend on the phase of the pathological process. The highest concentrations of MMP-8, MMP-9 and TIMP-1 are recorded in the acute phase of the disease. In the phase of early convalescence, 3–5 days after the remediation of the focus of inflammation, the concentrations of MMP-8, MMP-9 and TIMP-1 in serum are decreased, while the dynamics of decreasing of the content of MMP-8 and MMP-9 prevails over that for TIMP-1. The coefficients MMP-8/TIMP-1 and MMP-9/TIMP-1, which characterize the balance in the system of MMP/TIMP, allow assessing the state of the system in the dynamics of the disease.
References
Borysenko, A. V., Antonenko, M. Yu., Lynovytska, L. V., Sidelnikova, L. F., Nesyn, O. F., Dikova, I. H., … Skyba, I. M. (2017). Stomatolohichni zakhvoriuvannia: terapevtychna stomatolohiia [Dental treatment: therapeutic dentistry]. Kyiv: Medytsyna. [in Ukrainian].
Nazir, M. A. (2017). Prevalence of periodontal disease, its association with systemic diseases and prevention. International Journal of Health Sciences-Ijhs, 11(2), 72-80.
Righolt, A. J., Jevdjevic, M., Marcenes, W., & Listl, S. (2018). Global-, Regional-, and Country-Level Economic Impacts of Dental Diseases in 2015. Journal of Dental Research, 97(5), 501-507. https://doi.org/10.1177/0022034517750572
Tonetti, M. S., Jepsen, S., Jin, L. J., & Otomo-Corgel, J. (2017). Impact of the global burden of periodontal diseases on health, nutrition and wellbeing of mankind: A call for global action. Journal of Clinical Periodontology, 44(5), 456-462. https://doi.org/10.1111/jcpe.12732
Ziebolz, D., Schmalz, G., Kauffels, A., Widmer, F., Widmer, K., Slotta, J. E., . . . Kollmar, O. (2017). Periodontal pathogenic bacteria and aMMP-8 findings depending on periodontal conditions of patients before and after liver transplantation. Clinical Oral Investigations, 21(3), 745-752. https://doi.org/10.1007/s00784-016-1821-4
Kirchner, A., Jager, J., Krohn-Grimberghe, B., Patschan, S., Kottmann, T., Schmalz, G., . . . Ziebolz, D. (2017). Active matrix metalloproteinase-8 and periodontal bacteria depending on periodontal status in patients with rheumatoid arthritis. Journal of Periodontal Research, 52(4), 745-754. https://doi.org/10.1111/jre.12443
de Morais, E. F., Pinheiro, J. C., Leite, R. B., Santos, P. P. A., Barboza, C. A. G., & Freitas, R. A. (2018). Matrix metalloproteinase-8 levels in periodontal disease patients: A systematic review. Journal of Periodontal Research, 53(2), 156-163. https://doi.org/10.1111/jre.12495
Zhang, L., Li, X., Yan, H., & Huang, L. (2018). Salivary matrix metalloproteinase (MMP)-8 as a biomarker for periodontitis A PRISMA-compliant systematic review and meta-analysis. Medicine, 97(3). https://doi.org/10.1097/md.0000000000009642
Franco, C., Patricia, H. R., Timo, S., Claudia, B., & Marcela, H. (2017). Matrix Metalloproteinases as Regulators of Periodontal Inflammation. International Journal of Molecular Sciences, 18(2). https://doi.org/10.3390/ijms18020440
Jasberg, H., Tervahartiala, T., Sorsa, T., Soderling, E., & Haukioja, A. (2018). Probiotic intervention influences the salivary levels of Matrix Metalloproteinase (MMP)-9 and Tissue Inhibitor of metalloproteinases (TIMP)-1 in healthy adults. Archives of Oral Biology, 85, 58-63. https://doi.org/10.1016/j.archoralbio.2017.10.003
Bastos, M. F., Tucci, M. A., de Siqueira, A., de Faveri, M., Figueiredo, L. C., Vallim, P. C., & Duarte, P. M. (2017). Diabetes may affect the expression of matrix metalloproteinases and their inhibitors more than smoking in chronic periodontitis. Journal of Periodontal Research, 52(2), 292-299. https://doi.org/10.1111/jre.12394
Alfakry, H., Malle, E., Koyani, C. N., Pussinen, P. J., & Sorsa, T. (2016). Neutrophil proteolytic activation cascades: a possible mechanistic link between chronic periodontitis and coronary heart disease. Innate Immunity, 22(1), 85-99. https://doi.org/10.1177/1753425915617521
Koymans, K. J., Bisschop, A., Vughs, M. M., van Kessel, K. P. M., de Haas, C. J. C., & van Strijp, J. A. G. (2016). Staphylococcal Superantigen-Like Protein 1 and 5 (SSL1 & SSL5) Limit Neutrophil Chemotaxis and Migration through MMP-Inhibition. International Journal of Molecular Sciences, 17(7). https://doi.org/10.3390/ijms17071072
Ayravainen, L., Heikkinen, A. M., Kuuliala, A., Ahola, K., Koivuniemi, R., Moilanen, E., . . . Sorsa, T. (2018). Anti-rheumatic medication and salivary MMP-8, a biomarker for periodontal disease. Oral Diseases, 24(8), 1562-1571. https://doi.org/10.1111/odi.12930
Fernández, A., Cárdenas, A. M., Astorga, J., Veloso, P., Alvarado, A., Merino, P., . . . Hernández, M. (2019). Expression of toll-like receptors 2 and 4 and its association with matrix metalloproteinases in symptomatic and asymptomatic apical periodontitis. Clinical Oral Investigations, 23(12), 4205-4212. https://doi.org/10.1007/s00784-019-02861-9
Gursoy, U. K., Kononen, E., Tervahartiala, T., Gursoy, M., Pitkanen, J., Torvi, P., . . . Sorsa, T. (2018). Molecular forms and fragments of salivary MMP-8 in relation to periodontitis. Journal of Clinical Periodontology, 45(12), 1421-1428. https://doi.org/10.1111/jcpe.13024
Li, X. T., Lu, J. X., Teng, W., Zhao, C. J., & Ye, X. L. (2018). Quantitative Evaluation of MMP-9 and TIMP-1 Promoter Methylation in Chronic Periodontitis. DNA and Cell Biology, 37(3), 168-173. https://doi.org/10.1089/dna.2017.3948
Gao, Y., Hao, C. D. (2018). Expression of miR-146a in saliva of chronic periodontitis patients and its influence on gingival crevicular inflammation and MMP-8/TIMP-1 levels. Shanghai Kou Qiang Yi Xue. 27(3), 309-312.
Wang, L., Feng, K., & Sun, L. (2014). The influences of non-surgical periodontal treatment on gingival crevicular fluid levels of MMP-8 and tissue inhibitor of TIMP-1. Shanghai Kou Qiang Yi Xue, 23(2), 229-232.
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
