The balance of pro-inflammatory and anti-inflammatory cytokines in the fluid of the radicular cysts of the jaws in adults in asymptomatic and clinically manifest
DOI:
https://doi.org/10.14739/2310-1237.2020.1.203707Keywords:
radicular cysts, interleukins, tumor necrosis factor, inflammationAbstract
Aim. The aim of the work is to study the balance of pro-inflammatory and anti-inflammatory cytokines in the fluid of radicular jaw cysts in adults with asymptomatic and clinically manifest course of the disease.
Materials and methods. Cystic fluid was studied in 28 adult men aged 35-43 years with radicular cysts of the jaws, of which 11 patients had asymptomatic course of the disease, and 17 patients had clinically manifest course.
The concentrations of IL-1β, IL-4, IL-6, IL-8, IL-10, and TNF-α were determined by the enzyme-linked immunosorbent assay using the automatic enzyme-linked immunosorbent assay “GBG Star Fax 2100” from “Awareness Technology Inc.” (USA) with the help of commercial standard test systems of company “Gen-Probe Diaclone” (France). Statistical evaluation of the obtained results was carried out using formed database in the program Statistica v. 10.0 (StatSoft. Inc., USA), No. STA999K347156-W.
Results. It was found that in the asymptomatic course of the radicular jaw cysts, the concentrations of pro-inflammatory cytokines (IL-1β, IL-6, IL-8 and TNF-α) in the cystic fluid were significantly lower than the concentrations of anti-inflammatory cytokines (IL-4, IL-10).The concentration of IL-4 was 2.27 times higher than the concentration of IL-1β, 3.03 times higher than IL-6, and 2.81 and 2.51 times higher than IL-8 and TNF-α respectively. The concentration of IL-10 exceeded that for IL-1β by 1.56 times, for IL-6 - by 2.09 times, for IL-8 and TNF-α - by 1.95 and 1.73 times, respectively. All ratios between pro-inflammatory and anti-inflammatory cytokines, which characterized the balance in their systems, were less than 1, which indicated the predominance of anti-inflammatory cytokines over pro-inflammatory.
In the clinically manifest course of radicular cysts of the jaw, with the presence of symptoms of inflammation, the concentrations of pro-inflammatory cytokines in the cystic fluid increased significantly, while the increase in the concentrations of cytokines with anti-inflammatory action was relatively insignificant, and therefore there was a predominance of pro-inflammatory cytokines over anti-inflammatory.
Conclusions. Asymptomatic and clinical manifest course of the radicular cysts of the jaw have differences in the concentrations and balance of pro-inflammatory and anti-inflammatory cytokines in the composition of the cystic fluid. In the asymptomatic course of radicular cysts of the jaw, in the absence of symptoms of inflammation, the concentration of pro-inflammatory cytokines (IL-1β, IL-6, IL-8 and TNF-α) in the cystic fluid is significantly lower than the concentrations of anti-inflammatory cytokines (IL-4, IL-10). In the clinically manifest course of radicular cysts of the jaw, with the presence of symptoms of inflammation, in the cystic fluid, the concentrations of pro-inflammatory cytokines significantly increase and prevail over the concentrations of cytokines with anti-inflammatory effects.
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