Sex differences in the cardiac cholinergic response to adrenalin-induced myocardial necrosis and light desynchronosis

Authors

DOI:

https://doi.org/10.14739/2310-1237.2020.2.212727

Keywords:

necrosis, chronobiology disorders, cholinergic receptors, vagus nerve, sex

Abstract

 

The aim was to identify sex differences in the cardiac response to cholinergic stimuli in the development of myocardial necrosis and light desynchronosis.

Materials and methods. Experiments were conducted in 144 albino rats (72 males, 72 females). Myocardial necrosis was induced by adrenaline (0.5 mg/kg, intramuscularly).

The intensity of bradycardia that occurred as a response to intravenous acetylcholine injection and electrical stimulation of vagus nerve was measured. The animals were divided into 4 groups: 1 – rats that were in a light balance (day/night); 2 – rats exposed to light deprivation for 10 days; 3 – rats exposed to permanent lighting for 10 days; 4 – animals exposed to permanent lighting for 10 days and injected with melatonin (5 mg/kg) intraperitoneally 1 hour before the necrosis modeling.

Results. The development of myocardial necrosis when occurring in light deprivation was characterized by an increased responsiveness of the rat heart to cholinergic stimulation due to an increase in both cholinoreceptors sensitivity and release of acetylcholine from the vagus nerve terminals in females, and due to only an increased cholinoreceptors sensitivity in males. The development of myocardial necrosis in rats exposed to permanent lighting was characterized by significantly higher sensitivity of cholinergic structures of the heart than that in light balance, especially in females. Melatonin injection in the development of myocardial necrosis contributed to the heart cholinergic structures response to the stimulation, which was close to that observed in conditions of light balance, though it remained somewhat higher: due to the facilitation of acetylcholine release from vagus nerve in females, and it was combined with a higher sensitivity of cholinoreceptors in males.

Conclusions. The development of myocardial necrosis in light desynchronosis (light deprivation or permanent lighting) causes an increased heart response to cholinergic stimulation in rats. Such effects are stronger, especially in females in conditions of permanent lighting. The injection of melatonin in rats with myocardial necrosis and permanent lighting results in an approximation (but not restoration) of the heart sensitivity to cholinergic stimuli parameters to those that are observed in light balance. However, the heart response to cholinergic stimulation remains higher, especially in males.

 

References

Nicholas, M., Townsend, N., Scarborough, P., & Rayner, M. (2015). Corrigendum to: cardiovascular disease in Europe 2014: epidemiological update. European heart journal, 36(13), 794. https://doi.org/10.1093/eurheartj/ehu489

Roth, G. A., Forouzanfar, M. H., Moran, A. E., Barber, R., Nguyen, G., Feigin, V. L., Naghavi, M., Mensah, G. A., & Murray, C. J. (2015). Demographic and epidemiologic drivers of global cardiovascular mortality. The New England journal of medicine, 372(14), 1333-1341. https://doi.org/10.1056/NEJMoa1406656

EUGenMed Cardiovascular Clinical Study Group, Regitz-Zagrosek, V., Oertelt-Prigione, S., Prescott, E., Franconi, F., Gerdts, E., Foryst-Ludwig, A., Maas, A. H., Kautzky-Willer, A., Knappe-Wegner, D., Kintscher, U., Ladwig, K. H., Schenck-Gustafsson, K., & Stangl, V. (2016). Gender in cardiovascular diseases: impact on clinical manifestations, management, and outcomes. European heart journal, 37(1), 24-34. https://doi.org/10.1093/eurheartj/ehv598

Reitz, C. J., & Martino, T. A. (2015). Disruption of Circadian Rhythms and Sleep on Critical Illness and the Impact on Cardiovascular Events. Current Pharmaceutical Design, 21(24), 3505-3511. https://doi.org/10.2174/1381612821666150706105926

Fullagar, H., Skorski, S., Duffield, R., & Meyer, T. (2016). The effect of an acute sleep hygiene strategy following a late-night soccer match on recovery of players. Chronobiology International, 33(5), 490-505. https://doi.org/10.3109/07420528.2016.1149190

Gostyukhina, A. A., Zamoshchina, T. A., Zaitsev, K. V., Gutor, S. S., Zhukova, O. B., Svetlik, M. V., Abdulkina, N. G., & Zaitsev, A. A. (2018). Adaptivnye reaktsii krys posle svetovykh desinkhronozov i fizicheskogo pereutomleniya [Adaptive reactions of rats after light desynchronosis and physical overwork]. Bulletin of Siberian Medicine, 17(3), 22-34. [in Russian]. https://doi.org/10.20538/1682-0363-2018-3-22–34

Khara, M. R., & Shkumbatyuk, O. V. (2014). Polovye otlichiya reaktsii miokardial'nykh kholinoretseptorov pri povrezhdenii serdtsa adrenalinom na fone melatonina [Gender Difference of Myocardial Cholinergic Receptors Sensitivity at Adrenalin Heart Damage on the Ground of Melatonin]. Visnyk problem biolohii i medytsyny, (4), 176-179. [in Russian].

Yu, L. M., Di, W. C., Dong, X., Li, Z., Zhang, Y., Xue, X. D., Xu, Y. L., Zhang, J., Xiao, X., Han, J. S., Liu, Y., Yang, Y., & Wang, H. S. (2018). Melatonin protects diabetic heart against ischemia-reperfusion injury, role of membrane receptor-dependent cGMP-PKG activation. Biochimica Et Biophysica Acta-Molecular Basis of Disease, 1864(2), 563-578. https://doi.org/10.1016/j.bbadis.2017.11.023

Genade, S., Genis, A., Ytrehus, K., Huisamen, B., & Lochner, A. (2008). Melatonin receptor-mediated protection against myocardial ischaemia/reperfusion injury: role of its anti-adrenergic actions. Journal of Pineal Research, 45(4), 449-458. https://doi.org/10.1111/j.1600-079X.2008.00615.x

Green, E. A., Black, B. K., Biaggioni, I., Paranjape, S. Y., Bagai, K., Shibao, C., Okoye, M. C., Dupont, W. D., Robertson, D., & Raj, S. R. (2014). Melatonin Reduces Tachycardia in Postural Tachycardia Syndrome: A Randomized, Crossover Trial. Cardiovascular Therapeutics, 32(3), 105-112. https://doi.org/10.1111/1755-5922.12067

Campos, L., Cipolla-Neto, J., & Michelini, L. (2013). Melatonin modulates baroreflex control via area postrema. Brain And Behavior, 3(2), 171-177. https://doi.org/10.1002/brb3.123

Pyle, W. G., & Martino, T. A. (2018). Circadian rhythms influence cardiovascular disease differently in males and females: role of sex and gender. Current Opinion in Physiology, 5, 30-37. https://doi.org/10.1016/j.cophys.2018.05.003

Verkhovna Rada of Ukraine. (2006, February 21). Pro zakhyst tvaryn vid zhorstokoho povodzhennia ; zakon Ukrainy vid 21.02.2006 No. 3447-IV [On the Protection of Animals from Brutal Treatment (No. 3447-IV)]. https://zakon.rada.gov.ua/laws/show/3447-15?lang=en

Zaitsev, V. M., Liflyandskii, V. G., & Marinkin, V. I. (2006). Prikladnaya meditsinskaya statistika [Applied Medical Statistics]. St. Petersburg: Foliant. [in Russian].

Bezkorovaina, H. O., Klishch, I. M., & Khara, M. R. (2020). Statevi vidminnosti vilnoradykalnykh protsesiv u miokardi pry ushkodzhenni adrenalinom na tli svitlovoi depryvatsii [Gender difference of free-radical processes in the myocardium at adrenaline damage on the background of light deprivation]. Medical and Clinical Chemistry, (4), 13-19. [in Ukrainian]. https://doi.org/10.11603/mcch.2410-681X.2019.v.i4.10821

Khara, M. R., Kuziv, O. V., & Pelykh, V. Ye. (2015). Vplyv systemy oksydu azotu na chutlyvist cholinoretseptoriv sertsia pry poshkodzhenni adrenalinom v tvaryn zalezhno vid stati ta aktyvnosti honad [Effect of Nitric Oxide on Cholinireceptors of Heatr of Animals at Damage by Adrenalin in Terms of Sex and Activity of the Gonads]. ScienseRise, 5(4), 122-128. [in Ukrainian]. https://doi.org/https://doi.org/10.15587/2313-8416.2015.43305

Khara, M. R., & Kuchyrka, L. I. (2014). Rol melatoninu u kholiroreaktyvnosti miokarda honadektomovanykh shchuriv pry poshkodzhenni adrenalinom [Role of melatonin in holyroreactivity infarction in rats honadektomy damage adrenaline]. Aktualnye problemy transportnoi mediciny, 2(2), 21-26. [in Ukrainian].

ViviD, D., & Bentley, G. E. (2018). Seasonal Reproduction in Vertebrates: Melatonin Synthesis, Binding, and Functionality Using Tinbergen's Four Questions. Molecules, 23(3), Article 652. https://doi.org/10.3390/molecules23030652

Korkushko, O. V., & Pishak, V. P. (2012). V. I. Vernadskyi i khronorytmichna orhanizatsiia biosfery [V. I. Vernadskiy and chronorhythmic organization of the biosphere]. Krovoobih ta hemostaz, (4), 5-11. [in Ukrainian].

Malyarenko, Y. E., Malyarenko, T. N., Matyukhov, A. V., & Govsha, Y. A. (2001). Vegetativnoe obespechenie khronotropnoi funktsii serdtsa [The autonomic providing of the heart chronotropic function]. Vestnik TGU, 6(2), 230-240. [in Russian].

Chiglintsev, V. M. (2013). Vliyanie stimulyatsii bluzhdayushchikh nervov na pokazateli serdechnoi deyatel'nosti i soderzhanie oksida azota u rastushchikh gipokinezirovannykh i desimpatizirovannykh krys : dissertatsiya ... kandidata biologicheskikh nauk : 03.00.13 [Effect of vagus nerve stimulation on cardiac output and nitric oxide content in growing hypokinesis and desympathetic rats (PhD Dissertation)]. Tatar State Humanitarian Pedagogical University. [in Russian].

Khara, M. R., & Dorokhina, A. M. (2009). Vplyv honadektomii na stan systemy oksydu azotu v shchuriv riznoi stati [The effect of gonadectomy on the status of the system of nitric oxide in rats of different sexes]. Visnyk naukovykh doslidzshen, (3), 78-80. [in Ukrainian].

Dominguez-Rodriguez, A., Abreu-Gonzalez, P., Garcia-Gonzalez, M. J., Kaski, J. C., Reiter, R. J., & Jimenez-Sosa, A. (2007). A unicenter, randomized, double-blind, parallel-group, placebo-controlled study of Melatonin as an Adjunct in patients with acute myocaRdial Infarction undergoing primary Angioplasty - The Melatonin Adjunct in the acute myocaRdial Infarction treated with Angioplasty (MARIA) trial: Study design and rationale. Contemporary Clinical Trials, 28(4), 532-539. https://doi.org/10.1016/j.cct.2006.10.007

Reiter, R. J., & Tan, D. X. (2003). Melatonin: a novel protective agent against oxidative injury of the ischemic/reperfused heart. Cardiovascular Research, 58(1), 10-19. https://doi.org/10.1016/s0008-6363(02)00827-1

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1.
Khara MR, Bezkorovaina HO, Klishch IM, Pelykh VY. Sex differences in the cardiac cholinergic response to adrenalin-induced myocardial necrosis and light desynchronosis. Pathologia [Internet]. 2020Sep.28 [cited 2024Nov.23];(2). Available from: http://pat.zsmu.edu.ua/article/view/212727

Issue

No. 2 (2020)

Section

Original research

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