Features of the cytokine profile in the structures of chorion and placenta in premature termination of pregnancy
DOI:
https://doi.org/10.14739/2310-1237.2020.2.212790Keywords:
pregnancy, miscarriage, chorion, placenta, cytokines TNF-a, CD31/PECAM-1, CD45/T200/LCAAbstract
The placenta is a mirror of pregnancy. The formation of a healthy placenta predetermines embryo-endometrial relationships, which are regulated by cytokines. The study of the role of cytokines in the regulation of the gestation process in miscarriage is relevant.
The aim of the work is to establish markers of miscarriage based on the study of features of the cytokine profile in the structures of the chorion and placenta in premature termination of pregnancy.
Materials and methods. 22 chorions and 26 placentas from women with a history of reproductive loss and termination of current pregnancy were examined (the main group). The control was the tissue of the chorion, taken after artificial abortion in 20 women, and 20 placentas from uncomplicated pregnancy and physiological births in women with the first pregnancy. The placenta was examined according to the protocol (form No. 013-1/0). Expression of TNF-a, CD31/PECAM-1, CD45/T200/LCA in chorion and placenta structures were determined by immunohistochemistry.
Results. The termination of pregnancy in the first trimester is accompanied by a violation of the ratio of cytokines - an increase in the expression of TNF-α and a decrease in the expression of CD31/PECAM-1 in the structures of the endometrium and villous chorion. The results indicate a shift in the immunological balance towards an inflammatory response and a violation of the cytotrophoblastic invasion. Inflammation plays a leading role in the development of preterm birth. Structural and functional changes in the placenta against the background of inflammation make up the morphological substrate of chronic placental dysfunction, which, in a case of decompensation, triggers the process of preterm birth.
Conclusions. The cytokine marker of terminating pregnancy in the first trimester is the high expression of TNF-α in the structures of chorion and endometrium in combination with low expression of CD31/PECAM-1 in the endothelium of the spiral arteries, in the intravascular and interstitial cytotrophoblast. The marker of inflammation is a moderate expression of TNF- α in the invasive cytotrophoblast, decidual cells and terminal villi of placenta in combination with high expression of CD45/T200/LCA in the decidual membrane.
References
Zhivetskaya-Denisova, A. A., Vorobyova, I. I., Tkachenko, V. B., & Rudakova, N. V. (2017). Nevynoshuvannia vahitnosti: suchasni pidkhody do diahnostyky, profilaktyky ta likuvannia [Miscarriage of pregnancy: modern approaches to diagnostics, prevention, treatment]. Perinatologiya i pediatriya, (2), 91-98. [in Ukrainian]. https://doi.org/10.15574/pp.2017.70.91
Strizhakov, A. N., Belousova, V. S., & Svitich, O. A. (2016). Klinicheskoe znachenie Toll-podobnyh receptorov v patogeneze prezhdevremennyh rodov [Clinical significance of Toll-like receptors in the pathogenesis of preterm labor]. Voprosy ginekologii, akusherstva i perinatologii, 15(1), 35-40. [in Russian]. https://doi.org/10.20953/1726-1678-2016-1-35-40
Vorobyova, I. I., Skripchenko, N. I., Tkachenko, V. B., Rudakova, N. V., Zhyvetskaya-Denisova, A. A., & Tolkach, S. M. (2016). Vyznachennia roli polimorfizmu heniv-kandydativ u peredchasnomu pereryvanni vahitnosti riznykh terminiv [Defining the role of polymorphism of genes of different classes in premature termination of pregnancy by various terms]. Perinatologiya i pediatriya, (3), 61-65. [in Ukrainian].
Sementsova, N. A., Ponomareva, Ju. N., Barsanova, T. G., & Zayratyants, O. V. (2012). Narushenie protsessov angiogeneza, apoptoza i proliferatsii v tkanyakh plodnogo yaitsa i endometrii pri nerazvivayushcheisya beremennosti v I trimestre. [Disorders of angiogenesis, apoptosis and proliferation in the tissue of the gestational sac and endometrium in non-developing pregnancy]. Klinicheskaya i eksperimentalnaya morfologiya, (3), 11-16 [in Russian].
Lebedeva, О. P., Pakhomov, S. P., Ivashov, O. N., Startseva, N. Yu., & Churnosov, M. I. (2015). Signalnye receptory vrozhdennogo immuniteta v indukcii apoptoza pri nevynashivanii beremennosti rannih srokov [Signal receptors of congenital immunity in the induction of apoptosis in the case of miscarriages of early pregnancy]. Akusherstvo i ginekologiya, (2), 39-43 [in Russian].
Alijotas-Reig, J., Llurba, E., & Gris, J. M. (2014). Potentiating maternal immune tolerance in pregnancy: a new challenging role for regulatory T cells. Placenta, 35(4), 241-248. https://doi.org/10.1016/j.placenta.2014.02.004
Batrak, N. V., Malyshkina, A. I., & Kroshkina, N. V. (2014). Immunologicheskie aspekty privychnogo nevynashivaniya beremennosti [Immunological aspects of habitual miscarriage]. Akusherstvo i ginekologiya, (12), 10-14. [in Russian].
Jones, N. M., Holzman, C., Friderici, K. H., Jernigan, K., Chung, H., Wirth, J., & Fisher, R. (2010). Interplay of cytokine polymorphisms and bacterial vaginosis in the etiology of preterm delivery. Journal of reproductive immunology, 87(1-2), 82-89. https://doi.org/10.1016/j.jri.2010.06.158
Chernobai, L. V., Tyshchenko, A. N., Lazurenko, V. V., Muryzina, I. Yu., & Yurkova, O. V. (2016). Rol' narusheniya tsitokinovogo profilya v snizhenii fertil'nosti [The role of cytokine profile disruption in fertility reduction]. Mizhnarodnyi medychnyi zhurnal, (2), 38-43. [in Russian].
Yuan, J., Li, J., Huang, S. Y., & Sun, X. (2015). Characterization of the subsets of human NKT-like cells and the expression of Th1/Th2 cytokines in patients with unexplained recurrent spontaneous abortion. Journal of reproductive immunology, 110, 81-88. https://doi.org/10.1016/j.jri.2015.05.001
Dobrokhotova, Yu. E., Gankovskaya, L. V., Bakhareva, I. V., Svitich, O. A., Malushenko, S. V., & Magomedova, A. M. (2016). Rol' immunnykh mekhanizmov v patogeneze nevynashivaniya beremennosti [Role of immune mechanisms in the pathogenesis of miscarriage]. Akusherstvo i hinekolohiia, (7), 5-10. [in Russian]. http://doi.org/10.18565/aig.2016.7.5-10
Zhivetskaya-Denisova, A. A., Vorobyova, I. I., Tkachenko, V. B., Podolskyi, V. V., & Tykha, V. G. (2019). Platsenta yak dzerkalo vahitnosti (Ohliad literatury) [Placenta - mirror of pregnancy (literature review)]. Zdorov'e zhenshchiny, (3), 101-106. [in Ukrainian].
Antipkin, Yu. G., Zadorozhnaya, T. D., & Parnitskaya, O. I. (Eds.). (2016). Patologiya placenty (sovremennye aspekty) [Pathology of placenta: modern aspects]. Кyiv. [in Russian].
Gomez-Lopez, N., StLouis, D., Lehr, M. A., Sanchez-Rodriguez, E. N., & Arenas-Hernandez, M. (2014). Immune cells in term and preterm labor. Cellular & molecular immunology, 11(6), 571-581. https://doi.org/10.1038/cmi.2014.46
Azizieh, F. Y., & Raghupathy, R. G. (2015). Tumor necrosis factor-α and pregnancy complications: a prospective study. Medical principles and practice, 24(2), 165-170. https://doi.org/10.1159/000369363
Matson, B. C., & Caron, K. M. (2014). Uterine natural killer cells as modulators of the maternal-fetal vasculature. The International journal of developmental biology, 58(2-4), 199-204. https://doi.org/10.1387/ijdb.140032kc
Cöl-Madendag, I., Madendag, Y., Altinkaya, S. Ö., Bayramoglu, H., & Danisman, N. (2014). The role of VEGF and its receptors in the etiology of early pregnancy loss. Gynecological endocrinology, 30(2), 153-156. https://doi.org/10.3109/09513590.2013.864272
Okada, H., Tsuzuki, T., Shindoh, H., Nishigaki, A., Yasuda, K., & Kanzaki, H. (2014). Regulation of decidualization and angiogenesis in the human endometrium: mini review. The journal of obstetrics and gynaecology research, 40(5), 1180-1187. https://doi.org/10.1111/jog.12392
Peretiatko, L. P., Fateeva, N. V., Kuznetsov, R. A., & Malyshkina A. I. (2017). Vaskulyarizatsiya vorsin khoriona v pervom trimestre beremennosti pri fiziologicheskom techenii i privychnom nevynashivanii na fone khronicheskogo endometrita [Vascularization of chorion villi in the first trimester of gestation with physiological course and in recurrent miscarriage with underlying chronic endometritis]. I. P. Pavlov Russian Medical Biological Herald, 25(4), 612-620. [in Russian]. https://doi.org/10.23888/PAVLOVJ20174612-620
Brogin Moreli, J., Cirino Ruocco, A. M., Vernini, J. M., Rudge, M. V., & Calderon, I. M. (2012). Interleukin 10 and tumor necrosis factor-alpha in pregnancy: aspects of interest in clinical obstetrics. ISRN obstetrics and gynecology, 2012, 230742. https://doi.org/10.5402/2012/230742
Ashford, K., Chavan, N. R., Wiggins, A. T., Sayre, M. M., McCubbin, A., Critchfield, A. S., & O'Brien, J. (2018). Comparison of Serum and Cervical Cytokine Levels throughout Pregnancy between Preterm and Term Births. AJP reports, 8(2), e113-e120. https://doi.org/10.1055/s-0038-1656534
Shchegolev, A. I. (2016). Sovremennaya morfologicheskaya klassifikatsiya povrezhdenii platsenty [Current morphological classification of damages to the placenta]. Akusherstvo i ginekologiya, (4), 16-23. [in Russian]. http://doi.org/10.18565/aig.2016.4.16-23
Khong, T. Y., Mooney, E. E., Nikkels, P. G. J., Morgan, T. K., & Gordijn, S. J. (Eds.). (2019). Pathology of the Placenta: A Practical Guide. Switzerland: Springer Nature. https://doi.org/10.1007/978-3-319-97214-5
Vorobjova, I. I., Zhivetska-Denisova, A. A., Rudakova, N. V., Tkachenko, V. B., Tolkach, S. M., & Nakvasiuk, T. S. (2017). Osoblyvosti mistsevoho imunitetu u zhinok z nevynoshuvanniam [Features of local immunity in women with miscarriage]. Perinatologiya i pediatriya, (4), 49-53. [in Ukrainian].
Avtandilov, G. G. (1990). Medicinskaya morfometriya [Medical morphometry]. Moscow: Meditsina. [in Russian].
Putilova, N. V. (2011). Trombofilii i beremennost': prognozirovanie perinatal'nykh oslozhnenii i optimizatsiya taktiki vedeniya [Thrombophilia and pregnancy: prediction of perinatal complications and optimization of management tactics]. Akusherstvo i ginekologiya, (4), 31-35. [in Russian].
McQueen, D. B., Perfetto, C. O., Hazard, F. K., & Lathi, R. B. (2015). Pregnancy outcomes in women with chronic endometritis and recurrent pregnancy loss. Fertility and sterility, 104(4), 927-931. https://doi.org/10.1016/j.fertnstert.2015.06.044
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
