The effect of cytomegalovirus on clinical and paraclinical as well as immune parameters of children with rotavirus infection

Authors

DOI:

https://doi.org/10.14739/2310-1237.2021.2.230336

Keywords:

children, cytomegalovirus, rotavirus gastroenteritis

Abstract

The aim is to evaluate the effect of cytomegalovirus on clinical and paraclinical as well as immunological parameters in children with rotavirus infection (RVI).

Materials and methods. 50 children aged one to three years, patients with moderate and severe intestinal infections of rotavirus etiology were examined. All children were examined for herpes virus infections types 1, 2, 4, 5, 6. Enzyme-linked immunosorbent assay (ELISA) was used to determine the level of specific Ig G and Ig M for herpes viruses types 1, 2, 4, 5, 6, and in the case of a positive test, polymerase chain reaction (PCR) determined the presence of DNA (qualitatively) of these herpesviruses. Group 1 (mono-RVI), which was taken as a reference, included 33 children in whom no infection with any of these viruses of the herpes group was detected. Group 2 included 17 patients who underwent RVI on the background of CMV infection, the presence of infection with other viruses of the herpes group was excluded. Immune response parameters (CD 3+, CD 4+, CD 8+, CD 16+, CD 22+, Ig A, Ig M, Ig G, IL 1β, 4, TNF-α) in the acute period of the disease and in the period of convalescence were analyzed.

Qualitative indicators were given in the form of absolute and relative (%) value. Significance of differences was determined using Pearson’s χ2 test (Pearson’s Chi-squared test). Quantitative indicators were given in the form of median (Me) and values of lower (LQ) and upper (UQ) quartiles. The significance of differences in quantitative indicators in two unrelated groups was determined using the Mann–Whitney U-test. The threshold value of the confidence level was taken as 0.05.

Results. The analysis of the obtained data allows to establish that in the acute period of RVI infection in children with CMV leads to lower figures of temperature reaction, lower vomiting rate, prolongation of hospitalization, along with decrease in leukocytes, CD 16+ (%) cells and immunoregulatory index (CD 4+/CD 8+) against the background of increased levels of monocytes, CD 8+ (%) T-lymphocytes, concentrations of IL-4 and TNF-α. During convalescence, the presence of CMV is associated with an increase in the duration of fever and diarrhea, an increase in monocytes, CD 8+ (%) T cells, concentrations of IL-4, TNF-α and lower figures of immunoregulatory index, CD 16+ (%), CD 22+ (%) T cells and Ig M.

Conclusions. Latent cytomegalovirus infection in children with rotavirus gastroenteritis significantly affects a number of clinical and paraclinical as well as immune parameters, which leads to a decrease in the intensity of clinical manifestations in the acute period of the disease and the prolongation of some symptoms during convalescence.

Author Biographies

M. Yu. Sliepchenko, Kharkiv National Medical University, Ukraine

MD, Assistant of the Department of Pediatric Infectious Diseases

S. V. Kuznetsov, Kharkiv National Medical University, Ukraine

MD, PhD, DSc, Professor, Head of the Department of Pediatric Infectious Diseases

References

Badur, S., Öztürk, S., Pereira, P., AbdelGhany, M., Khalaf, M., Lagoubi, Y., Ozudogru, O., Hanif, K., & Saha, D. (2019). Systematic review of the rotavirus infection burden in the WHO-EMRO region. Human Vaccines and Immunotherapeutics, 15(11), 2754-2768. https://doi.org/10.1080/21645515.2019.1603984

Crawford, S. E., Ramani, S., Tate, J. E., Parashar, U. D., Svensson, L., Hagbom, M., Franco, M. A., Greenberg, H. B., O’Ryan, M., Kang, G., Desselberger, U., & Estes, M. K. (2017). Rotavirus infection. Nature Reviews Disease Primers, 3. https://doi.org/10.1038/nrdp.2017.83

Alain, S., Garnier-Geoffroy, F., Labrunie, A., Montané, A., Marin, B., Gatet, M., Grosjean, J., Dufour, V., Saugeras, M., Postil, D., & Hantz, S. (2020). Cytomegalovirus (CMV) Shedding in French Day-Care Centers: A Nationwide Study of Epidemiology, Risk Factors, Centers’ Practices, and Parents’ Awareness of CMV. Journal of the Pediatric Infectious Diseases Society, 9(6), 686-694. https://doi.org/10.1093/jpids/piz097

Martins-Celini, F. P., Yamamoto, A. Y., Passos, D. M., Do Nascimento, S. D., Lima, E. V., Di Giovanni, C. M., Quadrado, E. R. S., Barta, R., Aragon, D. C., Do Prado, S. I., De Almeida, M. F. B., & Mussi-Pinhata, M. M. (2016). Incidence, Risk Factors, and Morbidity of Acquired Postnatal Cytomegalovirus Infection among Preterm Infants Fed Maternal Milk in a HIg hly Seropositive Population. Clinical Infectious Diseases, 63(7), 929-936. https://doi.org/10.1093/cid/ciw394

Picarda, G., & Benedict, C. A. (2018). Cytomegalovirus: Shape-Shifting the Immune System. The Journal of Immunology, 200(12), 3881-3889. https://doi.org/10.4049/jimmunol.1800171

Van den Berg, S. P. H., Pardieck, I. N., Lanfermeijer, J., Sauce, D., Klenerman, P., van Baarle, D., & Arens, R. (2019). The hallmarks of CMV-specific CD8 T-cell differentiation. Medical Microbiology and Immunology, 208(3-4), 365-373. https://doi.org/10.1007/s00430-019-00608-7

Pachnio, A., Ciaurriz, M., Begum, J., Lal, N., Zuo, J., Beggs, A., & Moss, P. (2016). Cytomegalovirus Infection Leads to Development of High Frequencies of Cytotoxic Virus-Specific CD4+ T Cells Targeted to Vascular Endothelium. PLoS pathogens, 12(9), e1005832. https://doi.org/10.1371/journal.ppat.1005832

Müller, J., Tanner, R., Matsumiya, M., Snowden, M. A., Landry, B., Satti, I., Harris, S. A., O'Shea, M. K., Stockdale, L., Marsay, L., Chomka, A., Harrington-Kandt, R., Thomas, Z. M., Naranbhai, V., Stylianou, E., Mbandi, S. K., Hatherill, M., Hussey, G., Mahomed, H., Tameris, M., … Fletcher, H. A. (2019). Cytomegalovirus infection is a risk factor for tuberculosis disease in infants. JCI insight, 4(23), e130090. https://doi.org/10.1172/jci.insight.130090

Jain, S., Namdeo, D., Sahu, P., Kedia, S., Sahni, P., Das, P., Sharma, R., Gupta, V., Makharia, G., Dar, L., Travis, S. P., & Ahuja, V. (2020). High mucosal cytomegalovirus DNA helps predict adverse short-term outcome in acute severe ulcerative colitis. Intestinal research, 10.5217/ir.2020.00055. Advance online publication. https://doi.org/10.5217/ir.2020.00055

Bowyer, G., Sharpe, H., Venkatraman, N., Ndiaye, P. B., Wade, D., Brenner, N., Mentzer, A., Mair, C., Waterboer, T., Lambe, T., Dieye, T., Mboup, S., Hill, A., & Ewer, K. J. (2020). Reduced Ebola vaccine responses in CMV+ young adults is associated with expansion of CD57+KLRG1+ T cells. The Journal of experimental medicine, 217(7), e20200004. https://doi.org/10.1084/jem.20200004

Furman, D., Jojic, V., Sharma, S., Shen-Orr, S. S., Angel, C. J., Onengut-Gumuscu, S., Kidd, B. A., Maecker, H. T., Concannon, P., Dekker, C. L., Thomas, P. G., & Davis, M. M. (2015). Cytomegalovirus infection enhances the immune response to influenza. Science translational medicine, 7(281), 281ra43. https://doi.org/10.1126/scitranslmed.aaa2293

Usachova, E. V., Paholchuk, T. M., Silina, E. A., Matveeva, T. B., Shulga, O. V., Pechugina, V. V., Berestovaya, I. V., Shtibler, A. V., & Turlyun, V. A. (2013). Osoblyvosti perebihu rota virusnoi infektsii v ditei rannoho viku z tsytomehaliieiu ta pidkhody do patohenetychnoi terapii [Features of rotavirus infection course in infants with cytomegalovirus and the way of pathogenic therapy]. Sovremennaya pediatriya, (1), 134-138. [in Ukrainian].

Babik R. K. (2013). Kliniko-immunologicheskie osobennosti virusnykh kishechnykh infektsii u detei [Clinical and immunological features of viral intestinal infections in children]. Extended abstract of Doctor’s thesis. Moscow. [in Russian].

Klenerman, P., & Oxenius, A. (2016). T cell responses to cytomegalovirus. Nature reviews. Immunology, 16(6), 367-377. https://doi.org/10.1038/nri.2016.38

De Pelsmaeker, S., Romero, N., Vitale, M., & Favoreel, H. W. (2018). Herpesvirus Evasion of Natural Killer Cells. Journal of virology, 92(11), e02105-17. https://doi.org/10.1128/JVI.02105-17

Attaf, M., Roider, J., Malik, A., Rius Rafael, C., Dolton, G., Predergast, A. J., Leslie, A., Ndung'u, T., Kløverpris, H. N., Sewell, A. K., & Goulder, P. J. (2020). Cytomegalovirus-Mediated T Cell Receptor Repertoire Perturbation Is Present in Early Life. Frontiers in immunology, 11, 1587. https://doi.org/10.3389/fimmu.2020.01587

Boyarskaya, L. N., Usacheva, E. V., Gerasimchuk, T. S., Silina, E. A., Paholchuk, T. N., Konakova, O. V., Slipko, V. A., & Turlyun, V. A. (2014). Kliniko-imunolohichni osoblyvosti perebihu hostrykh respiratornykh infektsii u ditei rannoho viku, infikovanykh tsytomehalovirusom [Clinical and immunological features of acute respiratory infections in infants infected with cytomegalovirus]. Sovremennaya pediatriya, (1), 87-91. [in Ukrainian].

Bilovol, O. M., Kravchun, P. H., Babadzhan, V. D., Kuznetsova, L. V., Tsyhanenko, A. Y., Olkhovskyi, D. V., Kozhyn, M. I., Sydorov, O. P., Borzova, O. Y., Ryndina, N. H., & Tytova, H. Y. (2011). Klinichna imunolohiia ta alerholohiia [Clinical immunology and allergology], (рр. 38-71). Kharkiv: Grif. [in Ukrainian].

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Published

2021-08-20

How to Cite

1.
Sliepchenko MY, Kuznetsov SV. The effect of cytomegalovirus on clinical and paraclinical as well as immune parameters of children with rotavirus infection. Pathologia [Internet]. 2021Aug.20 [cited 2024Nov.22];18(2):211-7. Available from: http://pat.zsmu.edu.ua/article/view/230336

Issue

Vol. 18 No. 2 (2021): Pathologia

Section

Original research

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