Placental markers of miscarriage

Authors

  • A. A. Zhyvetska-Denysova State Institution “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine https://orcid.org/0000-0001-8418-5740
  • I. I. Vorobiova State Institution “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine https://orcid.org/0000-0002-6836-5845
  • N. Ya. Skrypchenko State Institution “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine https://orcid.org/0000-0003-2849-8499
  • S. M. Tolkach State Institution “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Academy of Medical Sciences of Ukraine”, Kyiv, Ukraine https://orcid.org/0000-0003-0753-8869
  • S. M. Razdaibedin Bogomolets National Medical University, Kyiv, Ukraine, Ukraine
  • Yu. M. Bondarenko SI “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Аcademy of Мedical Sciences of Ukraine”, Kyiv, Ukraine

DOI:

https://doi.org/10.14739/2310-1237.2021.3.232302

Keywords:

pregnancy, miscarriage, chorion, placenta, VEGF, CD31, PECAM1, CD105, endoglin, TGFβ 1/3 receptor, TNF-alpha, CD45/T200/LCA, CD56, NCAM1

Abstract

Successful implantation involves a high degree of development of spiral arteries, combined with high proliferative activity, which ensures the formation of a healthy placenta, full uterine-placental circulation, and the birth of a healthy child. The placenta is the unique organ of the biological monitoring, the mirror of pregnancy. Identification of placental markers of miscarriage is a promising direction for preventing reproductive losses.

The aim of the work is to identify the markers of miscarriage and premature labor in the structures of the chorion and placenta.

Materials and methods. The main group included tissue samples of the 22 chorions and 64 placentas after termination of current pregnancy from women with a history of reproductive losses. The control group included tissue samples of the 20 chorions after artificial abortion and 20 placentas after physiological pregnancy and birth. The placenta was examined according to the protocol (form No. 013-1/0). The expressions of VEGF, CD31/PECAM1, CD105/Endoglin/TGFβ 1/3 Receptor, Bcl-2α Ab-1, TNF-α, CD45/T200/LCA, CD56/NCAM1 were studied in the structures of chorion and placenta by immunohistochemistry.

Results. Based on histological and immuno-histochemical study of chorion and placenta samples in women with reproductive history and termination of the current pregnancy, it was established that embryo-endometrial dysfunction is the cause of miscarriage in the first trimester, and inflammation is the precondition of preterm birth; markers of miscarriage and premature labor in the structures of the chorion and placenta have been identified.

Conclusions. The markers of miscarriage are pathomorphological changes in endometrium and chorion combined with high expression of TNF-α and NK-CD56, low expression of CD31/PECAM1, negative expression of VEGF to indicate a violation of cytotrophoblast invasions. The markers of inflammation and premature labor are structural and functional changes of placenta in combination with moderate expression of TNF-α in syncytium, high expression of NK-CD56 in villous stroma, high expression of CD45/T200/LCA in the decidual membrane.

Author Biographies

A. A. Zhyvetska-Denysova, State Institution “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Academy of Medical Sciences of Ukraine”, Kyiv

MD, PhD, Senior Researcher of the Department of Scientific Problems of Miscarriage

I. I. Vorobiova, State Institution “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Academy of Medical Sciences of Ukraine”, Kyiv

MD, PhD, DSc, Head of the Department of Scientific Problems of Miscarriage

N. Ya. Skrypchenko, State Institution “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Academy of Medical Sciences of Ukraine”, Kyiv

MD, PhD, DSc, Head of the Department of Introduction and Research of Modern Medical Technologies Effectiveness in Obstetrics and Perinatology

S. M. Tolkach, State Institution “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Academy of Medical Sciences of Ukraine”, Kyiv

MD, PhD, Leading Researcher of the Department of Scientific Problems of Miscarriage

S. M. Razdaibedin, Bogomolets National Medical University, Kyiv, Ukraine

MD, PhD, Associate Professor of the Department of Dermatology and Venereology with a Course of Cosmetology

Yu. M. Bondarenko, SI “Institute of Pediatrics, Obstetrics and Gynecology named after Academician O. M. Lukyanova of National Аcademy of Мedical Sciences of Ukraine”, Kyiv

Junior Researcher, Laboratory of Pathomorphology

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Published

2021-12-01

How to Cite

1.
Zhyvetska-Denysova AA, Vorobiova II, Skrypchenko NY, Tolkach SM, Razdaibedin SM, Bondarenko YM. Placental markers of miscarriage. Pathologia [Internet]. 2021Dec.1 [cited 2024Dec.1];18(3):328-39. Available from: http://pat.zsmu.edu.ua/article/view/232302

Issue

Vol. 18 No. 3 (2021): Pathologia

Section

Original research

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