Cytokines, insulin resistance and arterial wall stiffness in assessing the course of non-alcoholic fatty liver disease

Authors

DOI:

https://doi.org/10.14739/2310-1237.2022.1.245985

Keywords:

fatty liver, arterial stiffness

Abstract

The progression of non-alcoholic fatty liver disease is associated with an increased prevalence of cardiovascular disease.

Aim: to assess the level of cytokines, insulin resistance, arterial wall stiffness during the progression of hepatic steatosis in patients with non-alcoholic fatty liver disease.

Material and methods. 87 patients with non-alcoholic fatty liver disease were examined. Group I consisted of 14 patients without steatosis, group II – 32 patients with steatosis grade 1; III – 30 patients with steatosis grade 2, IV group – 11 patients with steatosis grade 3. The control group consisted of 30 apparently healthy individuals. Steatometry with a quantitative assessment of the grade of hepatic steatosis was performed using a Soneus P7 ultrasound machine. The content of cytokines (interleukin-6, interleukin-10, TNF-α) was determined using an enzyme-linked immunosorbent assay using a Stat Fax 303 Plus analyzer. The level of insulin resistance was determined using the HOMA-IR index.

Results. The content of interleukin-6 in the blood serum of patients of group IV was significantly increased relative to the level in the control group (by 1.8 times, P < 0.05), patients of group I (by 3.2 times, P < 0.05), II group (by 3.0 times, P < 0.05) and group III (2.3 times, P < 0.05). The level of TNF-α increased with the progression of structural changes in the liver and at grade 4 of steatosis its content was increased by 6.4 times (P < 0.05) relative to the level in the control group, by 8.0 times (P < 0.05) relative to group I patients, by 3.4 times (P < 0.05) – group II patients and by 1.8 times (P < 0.05) – group III patients. An increase in the HOMA-IR index by 3.2 times (P < 0.05) was found in patients with moderate steatosis and 3.9 times (P < 0.05) in patients with severe steatosis compared to controls. A correlation was established between the intima-media thickness of the left common carotid artery and the level of TNF-α (r = 0.438; P = 0.006) and the HOMA-IR index (r = 0.557; P = 0.0008).

Conclusions. In patients with non-alcoholic fatty liver disease with progression of the grade of hepatic steatosis, a significant increase in proinflammatory cytokines, insulin resistance and intima-media thickness of the left common carotid artery was found.

Author Biographies

Yu. M. Stepanov, State Institution “Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine”, Dnipro

MD, PhD, DSc, Professor, Director of State Institution “Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine”

V. I. Didenko, State University “Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine”, Dnipro

MD, PhD, Senior Researcher, Deputy Director for Research

O. M. Tatarchuk, State Institution “Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine”, Dnipro

PhD, Senior Researcher of the Research Sector

I. S. Konenko, State Institution “Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine”, Dnipro

MD, PhD, Head of the Department of Mini-invasive Endoscopic Interventions and Instrumental Diagnostics

O. P. Petishko, State Institution “Institute of Gastroenterology of the National Academy of Medical Sciences of Ukraine”, Dnipro

Researcher of the Department of Scientific-Organizational, Methodical Work and Information Technologies

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Published

2022-04-15

How to Cite

1.
Stepanov YM, Didenko VI, Tatarchuk OM, Konenko IS, Petishko OP. Cytokines, insulin resistance and arterial wall stiffness in assessing the course of non-alcoholic fatty liver disease. Pathologia [Internet]. 2022Apr.15 [cited 2024Apr.16];19(1):5-11. Available from: http://pat.zsmu.edu.ua/article/view/245985

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Original research