Influence of shortwave laser therapy on morphometric parameters of sciatic nerve regeneration after experimental neurorrhaphy

Authors

DOI:

https://doi.org/10.14739/2310-1237.2023.3.282793

Keywords:

sciatic nerve, regeneration, morphometry, laser therapy

Abstract

The aim of this work was to reveal the effectiveness of delayed laser therapy for the regeneration of the sciatic nerve.

Materials and methods. In 21 rats, transection and epineural microsurgical suture of the right sciatic nerve (SN) were performed. In 16 rats (experimental group) in the period from 1.0 to 2.5 months after surgery, a course of laser therapy (LT-1, n = 8) and a delayed (after 6 months) course of laser therapy (LT-2, n = 8) were used 18 sessions were performed in each subgroup. Laser therapy was not performed in 5 operated animals and they were included in the control group. The terms of withdrawal of rats from the experiment were 2.5 and 12.0 months after the operation. During the morphometry of semi-thin sections distal to the level of the sutures, the volumetric density of the constituent elements of the SN, the number density of myelinated nerve fibers (MNF), their diameters, the thickness of the axons and myelin sheath, the myelination index (index G) distribution of MNF by diameter and dependence of index G on the fiber caliber were measured. For double control, we used muscle morphometry data from intact rats (Intact). Muscle tissue was additionally examined by the method of transmission electron microscopy using a generally accepted technique with a REM-100 electron microscope (Selmi, Sumy, Ukraine).

Results. In the control group, as compared to the intact group, the numerical density of muscle fibers was 21 % higher, but 38 % less than the numerical density of microvessels, therefore, the vascularization index was reduced by 1.7 times. The obtained results indicated laser compensation for denervation atrophy, which was confirmed by morphometric data.

Conclusions. The results of the study indicate a stimulating prolonged effect of delayed laser therapy in regenerative axonogenesis.

Author Biographies

Z. M. Yashchуshуn, Vasyl Stefanyk Precarpathian National University, Ivano-Frankivsk, Ukrainе

MD, PhD, Head of the Department of Physical Therapy, Occupational Therapy

O. O. Dyadyk, Shupyk National Healthcare University of Ukraine, Kyiv

MD, PhD, DSc, Professor, Head of Department of Pathological Anatomy and Forensic Medicine

References

Eberstein, A., & Eberstein, S. (1996). Electrical stimulation of denervated muscle: is it worthwhile?. Medicine and science in sports and exercise, 28(12), 1463-1469. https://doi.org/10.1097/00005768-199612000-00004

Schimrigk, K., McLaughlin, J., & Grüninger, W. (1977). The effect of electrical stimulation on the experimentally denervated rat muscle. Scandinavian journal of rehabilitation medicine, 9(2), 55-60.

Schwartz, L. M. (2008). Atrophy and programmed cell death of skeletal muscle. Cell death and differentiation, 15(7), 1163-1169. https://doi.org/10.1038/cdd.2008.68

Nakano, S. (2020). Histological investigation of picosecond laser-toning and fractional laser therapy. Laser therapy, 29(1), 53-60. https://doi.org/10.5978/islsm.20-OR-05

Bianciardi, G., Pontenani, F., Vassallo, L., Tacchini, D., Buonsanti, M., & Tripodi, S. (2016). Morphometric analysis of cryofixed muscular tissue for intraoperative consultation. Microscopy research and technique, 79(3), 155-161. https://doi.org/10.1002/jemt.22616

Ebrahimi-Zadehlou, P., Najafpour, A., & Mohammadi, R. (2021). Assessments of regenerative potential of silymarin nanoparticles loaded into chitosan conduit on peripheral nerve regeneration: a transected sciatic nerve model in rat. Neurological research, 43(2), 148-156. https://doi.org/10.1080/01616412.2020.1831341

Smith, J. J. (2017). Laser Therapy and Multimodal Postoperative Rehabilitation. In Laser Therapy in Veterinary Medicine (pp. 239-251). John Wiley & Sons, Inc. https://doi.org/10.1002/9781119220190.ch22

Bersch, I., & Fridén, J. (2021). Electrical stimulation alters muscle morphological properties in denervated upper limb muscles. EBioMedicine, 74. https://doi.org/10.1016/j.ebiom.2021.103737

Meijer, A. E. (1979). Semipermeable membrane techniques in quantitative enzyme histochemistry. Ciba Foundation Symposium, (73), 103-120. https://doi.org/10.1002/9780470720561.ch7https://search.crossref.org/?q=Enzyme+histochemistry&from_ui=yes

Durfee, W. K., & MacLean, K. E. (1989). Methods for estimating isometric recruitment curves of electrically stimulated muscle. IEEE transactions on bio-medical engineering, 36(7), 654-667. https://doi.org/10.1109/10.32097

Downloads

Published

2023-12-22

How to Cite

1.
Yashchуshуn ZM, Dyadyk OO. Influence of shortwave laser therapy on morphometric parameters of sciatic nerve regeneration after experimental neurorrhaphy. Pathologia [Internet]. 2023Dec.22 [cited 2024Oct.5];20(3):261-5. Available from: http://pat.zsmu.edu.ua/article/view/282793

Issue

Section

Original research