Dynamics of enzymatic and glutathione link of antioxidant protection of the liver of rats of different age in case of experimental cranioskeletal injury

Authors

DOI:

https://doi.org/10.14739/2310-1237.2024.2.302020

Keywords:

traumatic brain injury, skeletal injury, liver, oxidative stress, antioxidant system, superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione

Abstract

Aim. To determine the peculiarities of enzymatic and glutathione link of antioxidant protection of the liver of rats of different age in case of experimental cranioskeletal injury.

Materials and methods. Cranioskeletal trauma (CST) was modelled in rats of three age groups (sexually immature, sexually mature, old) under thiopental sodium anesthesia. Rats were removed from the experiment under anesthesia after 3 and 7 days of the post-traumatic period. Superoxide dismutase and catalase activity, as well as glutathione peroxidase (GP) and glutathione reductase (GR) activity and the content of reduced glutathione were determined in the liver homogenate extract.

Results. It was identified that, regardless of age, under the influence of CST, after 3 and 7 days of the post-traumatic period, in the liver of experimental rats, indicated parameters of enzymatic and glutathione link of antioxidant protection significantly decrease. However, the degree of decrease of the studied indicators is significantly greater in old rats, in particular after 7 days: according to catalase activity – on 30.8 % compared to sexually immature rats and on 27.0 % compared to sexually mature rats; according to glutathione peroxidase activity – on 31.6 % compared to sexually immature rats and on 23.5 % compared to sexually mature rats; according to glutathione reductase activity – on 22.0 % compared to sexually mature rats. The obtained results indicate a systemic pro-oxidant effect of the modelled injury on organs which are distant from the site of direct injury, as well as a decrease in the antioxidant capacity of the liver of rats with increasing age.

Conclusions. Under the influence of CST, the activity of the enzyme and glutathione link of antioxidant protection of the liver gradually decreases after 3 and 7 days in experimental rats, regardless of age. Under the conditions of CST modeling, the antioxidant capacity of the liver of old rats is lower than that of sexually mature and immature rats.

Author Biographies

Yu. I. Sushko, Danylo Halytsky Lviv National Medical University, Ukraine

PhD, Associate Professor of the Department of Normal Anatomy

M. I. Badіuk, Ukrainian Military Medical Academy, Kyiv

MD, PhD, DSc, Professor, Нead of the Department of Armed Forces Medical Support

A. A. Hudyma, Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

MD, PhD, DSc, Professor, Chief of the Department of Emergency and Simulation Medicine

O. A. Zachepa, Military Medical Clinical Center of Western region, Lviv, Ukraine

MD, PhD, Deputy Chief on Medical Affairs – Chief of the Department

A. V. Dobrorodnii, Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

MD, PhD, Associate Professor of Department of Anaesthesiology and Intensive Care

L. Ye. Hrytsyshyn, Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

MD, PhD, Associate Professor of Department of Infectious Diseases and Epidemiology, Dermatology and Venereology

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Additional Files

Published

2024-08-30

How to Cite

1.
Sushko YI, Badіuk MI, Hudyma AA, Zachepa OA, Dobrorodnii AV, Hrytsyshyn LY. Dynamics of enzymatic and glutathione link of antioxidant protection of the liver of rats of different age in case of experimental cranioskeletal injury. Pathologia [Internet]. 2024Aug.30 [cited 2024Nov.21];21(2):156-61. Available from: http://pat.zsmu.edu.ua/article/view/302020

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Original research