Dynamics of enzymatic and glutathione link of antioxidant protection of the liver of rats of different age in case of experimental cranioskeletal injury

Authors

DOI:

https://doi.org/10.14739/2310-1237.2024.2.302020

Keywords:

traumatic brain injury, skeletal injury, liver, oxidative stress, antioxidant system, superoxide dismutase, catalase, glutathione peroxidase, reduced glutathione

Abstract

Aim. To determine the peculiarities of enzymatic and glutathione link of antioxidant protection of the liver of rats of different age in case of experimental cranioskeletal injury.

Materials and methods. Cranioskeletal trauma (CST) was modelled in rats of three age groups (sexually immature, sexually mature, old) under thiopental sodium anesthesia. Rats were removed from the experiment under anesthesia after 3 and 7 days of the post-traumatic period. Superoxide dismutase and catalase activity, as well as glutathione peroxidase (GP) and glutathione reductase (GR) activity and the content of reduced glutathione were determined in the liver homogenate extract.

Results. It was identified that, regardless of age, under the influence of CST, after 3 and 7 days of the post-traumatic period, in the liver of experimental rats, indicated parameters of enzymatic and glutathione link of antioxidant protection significantly decrease. However, the degree of decrease of the studied indicators is significantly greater in old rats, in particular after 7 days: according to catalase activity – on 30.8 % compared to sexually immature rats and on 27.0 % compared to sexually mature rats; according to glutathione peroxidase activity – on 31.6 % compared to sexually immature rats and on 23.5 % compared to sexually mature rats; according to glutathione reductase activity – on 22.0 % compared to sexually mature rats. The obtained results indicate a systemic pro-oxidant effect of the modelled injury on organs which are distant from the site of direct injury, as well as a decrease in the antioxidant capacity of the liver of rats with increasing age.

Conclusions. Under the influence of CST, the activity of the enzyme and glutathione link of antioxidant protection of the liver gradually decreases after 3 and 7 days in experimental rats, regardless of age. Under the conditions of CST modeling, the antioxidant capacity of the liver of old rats is lower than that of sexually mature and immature rats.

Author Biographies

Yu. I. Sushko, Danylo Halytsky Lviv National Medical University, Ukraine

PhD, Associate Professor of the Department of Normal Anatomy

M. I. Badіuk, Ukrainian Military Medical Academy, Kyiv

MD, PhD, DSc, Professor, Нead of the Department of Armed Forces Medical Support

A. A. Hudyma, Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

MD, PhD, DSc, Professor, Chief of the Department of Emergency and Simulation Medicine

O. A. Zachepa, Military Medical Clinical Center of Western region, Lviv, Ukraine

MD, PhD, Deputy Chief on Medical Affairs – Chief of the Department

A. V. Dobrorodnii, Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

MD, PhD, Associate Professor of Department of Anaesthesiology and Intensive Care

L. Ye. Hrytsyshyn, Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine

MD, PhD, Associate Professor of Department of Infectious Diseases and Epidemiology, Dermatology and Venereology

References

Taylor CA, Bell JM, Breiding MJ, Xu L. Traumatic Brain Injury-Related Emergency Department Visits, Hospitalizations, and Deaths - United States, 2007 and 2013. MMWR Surveill Summ. 2017;66(9):1-16. doi: https://doi.org/https://doi.org/10.15585/mmwr.ss6609a1

de Vries R, Reininga IH, de Graaf MW, Heineman E, El Moumni M, Wendt KW. Older polytrauma: Mortality and complications. Injury. 2019;50(8):1440-7. doi: https://doi.org/https://doi.org/10.1016/j.injury.2019.06.024

McDonald SJ, Sun M, Agoston DV, Shultz SR. The effect of concomitant peripheral injury on traumatic brain injury pathobiology and outcome. J Neuroinflammation. 2016;13(1):90. doi: https://doi.org/https://doi.org/10.1186/s12974-016-0555-1

Antoni A, Heinz T, Leitgeb J. Polytrauma und begleitendes Schädel-Hirn-Trauma : Die Rolle des Unfallchirurgen [Polytrauma and concomitant traumatic brain injury : The role of the trauma surgeon]. Unfallchirurg. 2017;120(9):722-7. German. doi: https://doi.org/https://doi.org/10.1007/s00113-017-0354-x

Weihs V, Heel V, Dedeyan M, Lang NW, Frenzel S, Hajdu S, et al. Age and traumatic brain injury as prognostic factors for late-phase mortality in patients defined as polytrauma according to the New Berlin Definition: experiences from a level I trauma center. Arch Orthop Trauma Surg. 2021;141(10):1677-81. doi: https://doi.org/https://doi.org/10.1007/s00402-020-03626-w

Leichtle SW, Sarma AK, Strein M, Yajnik V, Rivet D, Sima A, et al. High-Dose Intravenous Ascorbic Acid: Ready for Prime Time in Traumatic Brain Injury? Neurocrit Care. 2020;32(1):333-9. doi: https://doi.org/https://doi.org/10.1007/s12028-019-00829-x

Davis CK, Vemuganti R. Antioxidant therapies in traumatic brain injury. Neurochem Int. 2022;152:105255. doi: https://doi.org/https://doi.org/10.1016/j.neuint.2021.105255

Ziablitsev SV, Yelskyi VM. Syndromy travmatychnoi khvoroby pry cherepno-mozkovii travmi [Syndromes of traumatic disease in traumatic brain injury]. Kramatorsk: Kashtan; 2020. Ukrainian.

Luca L, Rogobete AF, Bedreag OH. Oxidative Stress and Antioxidant Therapy in Critically Ill Polytrauma Patients with Severe Head Injury. The Journal of Critical Care Medicine. 2015;1(3):83-91. doi: https://doi.org/https://doi.org/10.1515/jccm-2015-0014

Sushko YI, Hudyma AA, Zachepa OA. [Influence of cranioskeletal trauma on the manifestations of cytolytic Syndrome in conditions of cranioskeletal trauma in rats of different ages]. Hospital Surgery. Journal named after LY. Kovalchuk. 2022;0(3):54-62. Ukrainian. doi: https://doi.org/https://doi.org/10.11603/2414-4533.2022.3.13393

Vlizla VV, editor. Laboratorni metody doslidzhennia u biolohii, tvarynnytstvi i veterynarnii medytsyn [Laboratory methods of research in biology, animal husbandry and veterinary medicine]. Lviv: SPOLOM; 2012. Ukrainian.

Sikirinskaya DA, Hudyma AA, Hospodarsky IY, Pokhodun KA. [Effect of cranioskeletal trauma complicated with blood loss on the activity of cytolysis and endogenous intoxication in the early period in rats with different hypoxia resistance]. Medychna ta klinichna khimiia. 2021;0(2):55-62. Ukrainian. doi: https://doi.org/https://doi.org/10.11603/mcch.2410-681X.2021.i2.12238

Mykhaylyuk IA, Gudyma AA. Dynamics of antioxident ptotection indices in answer to skeletal, cranial-cerebral trauma and combined traumas in the period of early signs of traumatic disease. Journal of Education, Health and Sport. 2015;5(4):29-40. doi: https://doi.org/https://doi.org/10.5281/zenodo.16591

Ali J, Aziz MA, Rashid MMO, Basher MA, Islam MS. Propagation of age-related diseases due to the changes of lipid peroxide and antioxidant levels in elderly people: A narrative review. Health Sci Rep. 2022;5(3):e650. doi: https://doi.org/https://doi.org/10.1002/hsr2.650

Stepanova HM. [Peculiarities of the course polytrauma at different ages]. Achievements of clinical and experimental medicine. 2015;0(2-3):18-20. Ukrainian. doi: https://doi.org/https://doi.org/10.11603/1811-2471.2015.v23.i2-3.5217

Shen Q, Hiebert JB, Hartwell J, Thimmesch AR, Pierce JD. Systematic Review of Traumatic Brain Injury and the Impact of Antioxidant Therapy on Clinical Outcomes. Worldviews Evid Based Nurs. 2016;13(5):380-9. doi: https://doi.org/https://doi.org/10.1111/wvn.12167

Additional Files

Published

2024-08-30

How to Cite

1.
Sushko YI, Badіuk MI, Hudyma AA, Zachepa OA, Dobrorodnii AV, Hrytsyshyn LY. Dynamics of enzymatic and glutathione link of antioxidant protection of the liver of rats of different age in case of experimental cranioskeletal injury. Pathologia [Internet]. 2024Aug.30 [cited 2024Nov.2];21(2):156-61. Available from: http://pat.zsmu.edu.ua/article/view/302020

Issue

Section

Original research