Quantitative indexes of stromal markers expression in the pancreatic ductal adenocarcinoma and chronic pancreatitis
DOI:
https://doi.org/10.14739/2310-1237.2015.2.48621Keywords:
Pancreas, Carcinoma, Pancreatitis, Stromal CellsAbstract
Differential diagnosis between pancreatic ductal adenocarcinoma and chronic pancreatitis with severe fibrosis of the stroma is often complicated by the fact that the rich desmoplastic tumor stroma imitates diffuse foci of fibrosis in pancreatitis.
Aim. In order to quantify the expression of stromal markers in desmoplastic stroma of pancreatic ductal adenocarcinoma and foci of severe fibrosis in chronic pancreatitis in 60 patients relative area of expression of α-SMA, desmin, fibronectin, and collagen types I and III was studied by immunohistochemistry and digital photomorphomety.
Results. There were significant differences in the quantitative expression of these markers in the desmoplastic stroma of ductal carcinoma and in areas of severe fibrosis in chronic pancreatitis, which can be used in the differential biopsy diagnosis of these diseases.
References
Tumanskij, V. A., & Kovalenko, I. S. (2013). Tyazhyolyj fibroz podzheludochnoj zhelezy pri khronicheskom pankreatite: osnovnye patomorfologicheskie sostavlyayushchie, immunofenotip fibrogennykh kletok i kollagena [Severe fibrosis of the pancreas in chronic pancreatitis: main pathological components, immunophenotype of fibrogenic cells and collagen]. Pathologia, 2013, 1(27), 27–30. [in Ukrainian].
Sinn, M., Denkert, C., Striefler, J. K., Pelzer, U., Stieler, J. M., Bahra, M., et al. (2014). α-Smooth muscle actin expression and desmoplastic stromal reaction in pancreatic cancer: results from the CONKO-001 study. British Journal of Cancer, 111, 1917–1923. doi: 10.1038/bjc.2014.495.
Brimienė, V., Brimas, G., & Strupas, K. (2011). Differential diagnosis between chronic pancreatitis and pancreatic cancer: a prospective study of 156 patients. Medicina (Kaunas), 47(3), 154–162.
Dabbs, D. J. (2010). Diagnostic Immunohistochemistry. New York: Ch. Livingstone.
Apte, M. V., Park, S., Phillips, P. A., Santucci, N., Goldstein, D., Kumar, R. K., et al. (2004). Desmoplastic reaction in pancreatic cancer. Role of pancreatic stellate cells. Pancreas, 29, 179–187. doi: 10.1097/00006676-200410000-00002.
Korc, M. (2007) Pancreatic cancer associated stroma production. American Journal of Surgery, 194(4, Suppl. 1), 84–86. doi: 10.1016/j.amjsurg.2007.05.004.
Neoptolemos, J. P., Urrutia, R., Abbruzzese, J. L., Büchler, M. W. (Eds.) (2010). Pancreatic Cancer. Springer Science+Business Media, LLC.
García-Pravia, C., Galván, J. A., Gutiérrez-Corral, N., Solar-García, L., García-Pérez, E., García-Ocaña, M., et al. (2013). Overexpression of COL11A1 by cancer-associated fibroblasts: clinical relevance of a stromal marker in pancreatic cancer. PLoS ONE, 8(10), e78327. doi:10.1371/journal.pone.0078327.
Rucki, A. A., & Zheng, L. (2014). Pancreatic cancer stroma: understanding biology leads to new therapeutic strategies. World Journal of Gastroenterology, 20(9), 2237–2246. doi: 10.3748/wjg.v20.i9.2237.
Neesse, A., Michl, P., Frese, K. K., Feig, C., Cook, N., Jacobetz, M.A., et al. (2011). Stromal biology and therapy in pancreatic cancer. Gut, 60, 861–868. doi: 10.1136/gut.2010.226092.
Erkan, M., Hausmann, S., Michalski, C. W., Fingerle, A. A., Dobritz, M., Kleeff, J., & Friess H. (2012). The role of stroma in pancreatic cancer: diagnostic and therapeutic implications. Nature Reviews Gastroenterology and Hepatology, 9, 454–467. doi: 10.1038/nrgastro.2012.115.
Klöppel, G., Hruban, R. H., Longnecker, D. S., et al. (2000). Tumours of the exocrine pancreas. World Health Organization Classification of Tumours-Pathology and Genetics of Tumours of the Digestive System. S. Hamilton, L. A. Aaltonen (Eds), (pp. 219–305). Lyon: IARC Press.
Downloads
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
