Immunohistochemical features of progesterone receptors expression of placental barrier in women with multiple pregnancies resulting from assisted reproduction

Authors

  • T. D. Zadorozhna SI «Institute of Pediatrics, Obstetrics and Gynecology of National Academy of Medical Sciences of Ukraine»,
  • B. M . Ventskivsky Bogomolets National Medical University,
  • S. M. Kylyhevich SI «Institute of Pediatrics, Obstetrics and Gynecology of National Academy of Medical Sciences of Ukraine»,
  • I. V. Poladich Bogomolets National Medical University,

DOI:

https://doi.org/10.14739/2310-1237.2016.1.71183

Keywords:

Progesterone Receptors, Placenta, Immunohistochemistry, Multiple Pregnancy, Assisted Reproductive Technology

Abstract

Hormonal disorders are one of the main known causes of miscarriage and preterm birth in multiple pregnancies resulting from assisted reproductive technology (ART). Progesterone and the number of its receptors play an important role in the preservation and prolongation of pregnancy and it is the pressing issue of our time. The study of placentas, as the main site of synthesis of progesterone, has high informative potential and it is the most important diagnostic object, and information received by its research is essential for the full conclusion on the causes, mechanisms, close and long-term effects of multiple pregnancy pathology.

Aim. The aim of our study was to investigate immunohistochemical features of placentas from women with dichorionic diamniotic twin pregnancies in spontaneous fertilization and after use of assisted reproductive technology (ART).

Methods and results.  According to this goal we examined 94 women, 44 of whom had multiple pregnancies due to ART, 42 with separate multiple pregnancy and 38 women with a singleton pregnancy. We carried out clinical and statistical analysis of the course of pregnancy and childbirth in the studied groups.

During the study it was found that multiple pregnancies due to assisted reproduction belong to the high risk of gestation, at which premature births occur much more frequently than in singleton pregnancies. We were the first to carry out the immunohistochemical study of placentas in which the highest expression of progesterone receptors in the nuclei of cells of decidua (45%) related to the parent structure of the placenta from women with multiple pregnancies caused by ART is found. It is also found that with increasing gestational age, there has been a significant decrease in the expression of the activity of progesterone receptors (from 45 to 2.5%), regardless of the method of conception and the number of fetuses.

Conclusions. The results of the study point to the definitive link of structures of placental-endometrial relations as an important component of the appropriateness of hormone therapy.

References

Arzhanova, O. N., & Pajkacheva, Yu. M. (2009) Gormonal'naya podderzhka mnogoplodnoj beremennosti [Hormonal support multiple pregnancy]. Zhurnal akusherstva i zhenskikh boleznej, LVIII (5), 96–97. [in Russian].

Boichuk A. H., Vakaliuk, L. M., Drohomyretskyi L. V., Liubіnets, V. O., & Yunger, V. І. (2013) Osoblyvosti perebihu bahatoplidnoi vahitnosti pislia zastosuvannia dopomizhnykh reproduktyvnykh tekhnolohii [Pregnancy outcomes after assisted reproductive technology]. Medyko-sotsialni problemy simi, 4, 5–8. [in Ukrainian].

Zharkich, A. V., Lubomirska, E. S., Carpenter, V. A., & Babinchuk, E. V. (2013) Problema nevynashivaniya pri mnogoplodnoj beremennosti [Рroblem of miscarriage in multiple pregnancies]. Zaporozhskij medicinskij zhurnal, 4(79), 78–80. [in Ukrainian].

Kamіnskyi, V. V., Sukhanova, A. A., Sparrow, L. І., et al. (2009) Bahatoplidna vahitnist pislia zastosuvannia dopomizhnykh reproduktyvnykh tekhnolohii. Osoblyvosti perebihu vahitnostei iz samovilnoiu ta shtuchnoiu reduktsiieiu embrioniv [Multiple pregnancy after assisted reproductive technology. Characteristics of spontaneous pregnancies and artificial reduction of embryos]. Zdorov'e zhenschiny, 6(42), 34–40. [in Ukrainian].

Markovych, T. Ye., & Zarytskyi, O. O. (2015) Analiz perebihu hestatsiinoho periodu ta uskladnen pry bahatoplidnii vahitnosti [Analysis of the gestational period and complications of multiple pregnancy]. Aktualni pytannia teoretychnoi ta praktychnoi medytsyny Proceedings of the 3rd International Scientific Conference, N.V. Demіhova (Ed.), (S. 329). Sumy: SumDU. [in Ukrainian].

Menshikova, V. V. (2009) Obespechenie kachestva laboratornykh issledovanij. Preanaliticheskij e′tap [Laboratory quality assurance. Preanalytical phase]. Moscow: Znanie. [in Russian].

Franka, G. A., & Mal'kova, P. G. (Eds.) (2011) Immunogistokhimicheskie metody: Rukovodstvo. Moscow. [in Russian].

Savel'eva, G. M., Kareva, E. N., Kramarenko, M. P., Kappusheva, L. M., Klimenko, P. A., & Suknovalova, M. V. (2011) Sostoyanie recepcii zhenskikh polovykh gormonov i e′ffektivnost' e′kstrakorporal'nogo oplodotvoreniya [The state of reception of female sex hormones and the efficacy of in-vitro fertilization]. Voprosy ginekologii, akusherstva i perinatologii, 10(1), 24–28. [in Russian].

Suknovalova, M. V., Klimenko (Kramarenko), M. P., & Karev, E. N. (2011) Svyazyvayuschie svojstva receptorov progesterona i e′stradiola s Dyufastonom u pacientok vklyuchennykh v programmu e′kstrakorporal'nogo oplodotvoreniya [The binding properties of progesterone and estradiol receptors with Duphaston in patients included in the program of in vitro fertilization]. Vestnik Rossijskogo gosudarstvennogo medicinskogo universiteta. Special'nyj vypusk, 1, 217. [in Russian].

Shherbyna, M. O., Kuzmina, I. Yu., & Plakhotna, I. Yu. (2015) Bahatoplidna vahitnist : metodychni vkazivky dlia samostiinoi roboty studentiv [Multiple pregnancy: guidelines for students]. Kharkіv: HNMU. [in Ukrainian].

Gonzalez, R. (2009) Prenatal priznachennya progesterone for Poperedjennia peredchasnih pologіv Sered zhіnok Yakimov zagrozhuyut peredchasnі canopies. RHL comment (stop redaktsіya 1 breast-2009r.). Geneva: Vsesvіtnya organіzatsіya receptionists Health Protection.

Ohta, Y., Sato, T., & Iguchi, T. (1993) Immunocytochemical Localization of Progesterone Receptor in the Reproductive Tract of Adult. Female Rats. Biology of reproduction, 48, 205–213.

Szekeres-Bartho, J. (2001) Progesterone-receptor mediated immunomodulation and anti-abortive effects. The role of progesterone induced Blocking Factor (PIBF): IX World congress of gynec. Endocrinology. Solvey Pharmacenticals Satelite Symposium, 4 Dec., 2001.

Downloads

How to Cite

1.
Zadorozhna TD, Ventskivsky BM ., Kylyhevich SM, Poladich IV. Immunohistochemical features of progesterone receptors expression of placental barrier in women with multiple pregnancies resulting from assisted reproduction. Pathologia [Internet]. 2016Jun.23 [cited 2024Nov.27];(1). Available from: http://pat.zsmu.edu.ua/article/view/71183

Issue

No. 1 (2016)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Лицензия Creative Commons
    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).