Immunohistochemical changes of maxillary sinus mucosa with underlying adenomatous polyposis
DOI:
https://doi.org/10.14739/2310-1237.2017.1.97793Keywords:
maxillary sinusitis, adenomatous polyp, subpopulation of T-lymphocytes, macrophages, plasma cellsAbstract
Introduction
In maxillary sinusitis pathogenesis researchers paid much attention to the role of paranasal sinuses, sinus drainage dysfunctions, mucocyliary transport system disorders in cases of damage of sinuses mucous membranes. Pathohistological, immunohistochemical and morphometric studies of mucosa were conducted. However, to evaluate the local inflammatory response in cases of chronic sinusitis and improvement of individual therapy for the patients, the immunohistochemical studies of the composition of inflammatory immunocyte infiltrates of paranasal sinus mucosa and adenoid vegetations bioptate would be reasonable.
The aim of the research was to study the immunohistochemical content of immunocyte infiltrates of paranasal sinuses adenomatous polyps in biopsy samples of patients suffering from chronic sinusitis.
Materials and Methods. Paranasal sinuses adenomatous polyps removed during surgery of 22 patients suffering from chronic maxillary sinusitis were studied. Immunohistochemical studies of paraffin sections of the removed polyps were performed using appropriate primary antibodies and visualization systems DAKO EnVision+ System (DAKO, Denmark).
Results. During microscopic investigation of paranasal sinuses adenomatous polyps of the patients suffering from chronic sinusitis, moderate and severe immunocyte infiltration of polyp stroma sometimes with focal follicular structures was presented. Immunohistochemical investigation of the cellular composition of these infiltrates proved the presence of CD8+ T-killer cells, CD4+ T-helper cells, CD68+ macrophages as well as single plasma cells. The irregularity of these cells markers expression was determined in dependence of the severity of inflammatory cell infiltration.
Conclusions. In the patients with chronic maxillary polyp sinusitis with mild and moderate immunocyte infiltration of paranasal sinus mucosa, among immune cells the CD4+ T-lymphocytes occupied the most relative area in polyp stroma, plasma cells – the least (р < 0.001). In the patients with significant immunocellular infiltration in immunocyte follicular infiltration of polyp stroma the relative area of CD4+ and CD8+ T-lymphocytes significantly exceeded the relative area of macrophages and plasma cells.
References
Betlejewski, S., & Betlejewski, A. (2008) The influence of nasal flow aerodynamics on the nasal physiology. Otolaryngol, 62(3), 321–325. doi: 10.1016/S0030-6657(08)70263-4.
Zavalii, M. A., & Bezshapochny, S. D. (2010) Rezulʹtaty kompleksnogo lecheniya bolʹnykh s obostreniem khronicheskogo gnojnogo sinusita [Results of complex treatment for patients suffering from exacerbation of chronic purulent sinusitis]. Zhurnal vushnykh, nosovykh i horlovykh khvorob, 6, 16–21. [in Russian].
Meusel, Т., Negoias, S., Scheibe, M., & Hummel, T. (2010) Topographical differences in distribution and responsiveness of trigeminal sensitivity within the human nasal mucosa. Pain, 151(2), 516–521. doi: 10.1016/j.pain.2010.08.013.
Ramadan, H. H., & Makary, C. A. (2014) Can computed tomography score predict outcome of adenoidectomy for chronic rhinosinusitis in children. The American Journal of Rhinology & Allergy, 28(1), 80–82. doi: 10.2500/ajra.2014.28.4004.
Evcimik, M. F., Dogru, M., Cirik, A. A., & Nepesov, M. I. (2015) Adenoid hypertrophy in children with allergic disease and influential factors. International Journal of Pediatric Otorhinolaryngology, 79(5), 694–697. doi: 10.1016/j.ijporl.2015.02.017.
Carvalho, F. G., Puppin-Rontani, R. M., Fúcio, S. B., & Negrini, T. C.; Carlo, H. L., Garcia-Godoy, F. (2012) Analysis by confocal laser scanning microscopy of the MDPB bactericidal effecton S. mutans biofilm CLSM analysis of MDPB bactericidal effect on biofilm. Journal of Applied Oral Science, 20(5), 568–575. doi: 10.1590/S1678-77572012000500013.
Pukhlik, S. M., & Buchatsky, M. S. (2013) Sovremennye metody vyzualizacii sostoyaniya okolonosovykh pazukh. Opyt ulʹtrazvukovoj diagnostiki [Modern methods of visualisation of paranasal sinuses. Experience of ultrasonic diagnostics]. Rynolohiya, 2, 58–64. [in Russian].
Zakharova, H. P., & Ilʹinskaya, E. V. (2002) Kharakteristika ulʹtrastrukturnykh osobennostej slizistoj obolochki verkhnechelyustnykh pazukh pri khronicheskikh rinosinusitakh [Characteristic ultrastructural features of the mucosa of the maxillary sinus in chronic rinosinuita]. Novosti otorinolaringologii, 3, 25–31. [in Russian].
Tan, B. K., Li, Q.-Zh., Suh, L., Kato, A., Conley, D. B., Chandra, R. K., et al. (2011). Evidence for intranasal anti-nuclear autoantibodies in Chronic Rhinosinusitis with Nasal Polyps. J Allergy Clin Immunol, 128(6), 1198–1206. doi: 10.1016/j.jaci.2011.08.037.
Hulse, K. E., Stevens, W. W., Tan, B. K., & Schleimer, R. P. (2015). Pathogenesis of nasal polyposis. Clin Exp Allergy, 45(2), 328–346. doi: 10.1111/cea.12472.
Dutsch-Wicherek, M., Tomaszewska, R., Lazar, A., Stręk, P., Wicherek, L., Piekutowski, K., & Jóźwicki, W. (2010). The evaluation of metallothionein expression in nasal polyps with respect to immune cell presence and activity. BMC Immunol., 11, 10. doi: 10.1186/1471-2172-11-10.
Claeys, S., De Belder, T., Holtappels, G., Gevaert, P., Verhasselt, B., Van Cauvenberge, P., Bachert, C. (2004). Macrophage mannose receptor in chronic sinus disease. Allergy; 59, 606–612. doi: 10.1111/j.1398-9995.2004.00471.x.
Könnecke, M., Böscke, R., Waldmann, A., Bruchhage, K.-L., Linke, R., Pries, R., et al. (2014). Immune Imbalance in Nasal Polyps of Caucasian Chronic Rhinosinusitis Patients Is Associated with a Downregulation of E-Selectin. J Immunol Res., 2014, 959854. doi: 10.1155/2014/959854.
Fokkens, W., Lund, V., & Mullol, J. (2007). European Position Paper on Rhinosinusitis and Nasal Polyps Group. Rhino, 20, 1–136.
Gevaert, P., Holtappels, G., Johansson, S.G.O., Cuvelier, C., Van Cauwenberge, P., & Bachert, C. (2005). Organisation of secondary lymphoid tissue and local IgE formation to Staphylococcus aureus enterotoxins in nasal polyps. Allergy, 60(1), 71–79. doi: 10.1111/j.1398-9995.2004.00621.x.
Dutsch-Wicherek, M., Tomaszewska, R., Strek, P., Wicherek, L., & Skladzien, J. (2006). The analysis of RCAS1 and DFF-45 expression in nasal polyps with respect to immune cells infiltration. BMC Immunol., 21, 7–4. doi: 10.1186/1471-2172-7-4.
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).
