The activity of markers of oxidative and nitrosative stresses in blood plasma of Parkinson’s disease patients at the early stages

Authors

DOI:

https://doi.org/10.14739/2310-1237.2021.2.233431

Keywords:

Parkinson’s disease, oxidative stress, nitrosative stress

Abstract

The aim of the study is to investigate activity of markers of oxidative and nitrosative stresses in blood plasma of patients in the I–II stages of Parkinson’s disease (PD) and to determine correlations between their concentrations and severity of non-motor PD symptoms.

Materials and methods. 67 patients at I–II PD stages and 20 healthy controls took part in the research. Cognitive functions were examined due to the Montreal Cognitive Assessment test – MoCA test. For the severity of psycho-emotional disorders evaluation the following scales and questionnaires were used: Night Sleep Assessment Questionnaire by A. M. Vein, Zung test for anxiety, apathy Starkstein scale, Boston stress-resistance test, Beck Depression Inventory (BDI-II). We performed ELISA test for determination of glutathione peroxidase (GPx) and glutathione-S-transferase (GST) activities and 3-nitrotyrosine (3-NT) level in blood plasma of participants (Elabscience® kit).

Results. The middle age of PD patients and healthy controls was 64.35 ± 1.22 and 66.40 ± 0.70 years, respectively. GPx activity in plasma of patients at І–ІІ PD stages was significantly lower than in healthy controls (P < 0.001) and was higher at the I stage compared to the II PD stage (P = 0.003). Also GPx activity in PD patients with normal cognition was higher than in PD patients with cognitive impairment (P = 0.042).

The GST activity in plasma of PD patients with anxiety was significantly lower (P = 0.002) compared to those without anxiety, and 3-NT blood plasma level in PD patients with moderate anxiety was higher than in those without one (P = 0.029).

Conclusions. The activity of antioxidant GPx was significantly lower in PD patients at early stages compared to healthy controls, and in PD patients in the II stage of the disease compared to the I stage, and it was significantly lower in PD patients with cognitive impairment. PD patients with moderate anxiety had lower 3-NT levels and GST activity in blood plasma.

Author Biographies

A. V. Demchenko, Zaporizhzhia State Medical University, Ukraine

MD, PhD, DSc, Associate Professor of the Department of Family Medicine, Therapy, Cardiology and Neurology of FPE

V. V. Biriuk, Zaporizhzhia State Medical University, Ukraine

MD, PhD-student of the Department of Family Medicine, Therapy, Cardiology and Neurology of FPE

A. V. Abramov, Zaporizhzhia State Medical University, Ukraine

MD, PhD, DSc, Professor of the Department of Pathological Physiology with Course of Normal Physiology

References

  1. Musgrove, R. E., Helwig, M., Bae, E. J., Aboutalebi, H., Lee, S. J., Ulusoy, A., & Di Monte, D. A. (2019). Oxidative stress in vagal neurons promotes parkinsonian pathology and intercellular α-synuclein transfer. The Journal of clinical investigation, 129(9), 3738-3753. https://doi.org/10.1172/JCI127330
  2. Llagostera-Reverter, I., López-Alemany, M., Sanz-Forner, R., González-Chordá, V., & Orts-Cortés, M. (2019). Quality of life and self-care in patients with Parkinson in a regional hospital: descriptive study. Enfermería Global, (53), 360-372. http://dx.doi.org/10.6018/eglobal.18.1.294561
  3. Zahoor, I., Shafi, A., & Haq, E. (2018). Pharmacological Treatment of Parkinson’s Disease. In T. B. Stoker, J. C. Greenland (Eds.), Parkinson’S Disease: Pathogenesis and Clinical Aspects (Ch 7, pp. 129-144). https://doi.org/10.15586/codonpublications.parkinsonsdisease.2018.ch7
  4. Ortiz, G. G., Pacheco-Moisés, F. P., Mireles-Ramírez, M. A., Flores-Alvarado, L. J., González-Usigli, H., Sánchez-López, A. L., Sánchez-Romero, L., Velázquez-Brizuela, I. E., González-Renovato, E. D., & Torres-Sánchez, E. D. (2016). Oxidative Stress and Parkinson’s Disease: Effects on Environmental Toxicology. In Rizwan Ahmad, Free Radicals and Diseases. https://doi.org/10.5772/63794
  5. Rizor, A., Pajarillo, E., Johnson, J., Aschner, M., & Lee, E. (2019). Astrocytic Oxidative/Nitrosative Stress Contributes to Parkinson's Disease Pathogenesis: The Dual Role of Reactive Astrocytes. Antioxidants, 8(8), 265. https://doi.org/10.3390/antiox8080265
  6. Blesa, J., Trigo-Damas, I., Quiroga-Varela, A., & Jackson-Lewis, V. R. (2015). Oxidative stress and Parkinson’s disease. Frontiers in Neuroanatomy, 9, 91. https://doi.org/10.3389/fnana.2015.00091
  7. Hermanowicz, N., Jones, S. A., & Hauser, R. A. (2019). Impact of non-motor symptoms in Parkinson's disease: a PMDAlliance survey. Neuropsychiatric disease and treatment, 15, 2205-2212. https://doi.org/10.2147/NDT.S213917
  8. Ragab, O. A., Elheneedy, Y. A., & Bahnasy, W. S. (2019). Non-motor symptoms in newly diagnosed Parkinson’s disease patients. The Egyptian Journal of Neurology, Psychiatry and Neurosurgery, 55, 24. https://doi.org/10.1186/s41983-019-0070-2
  9. Takamatsu, Y., Fujita, M., Ho, G. J., Wada, R., Sugama, S., Takenouchi, T., Waragai, M., Masliah, E., & Hashimoto, M. (2018). Motor and Nonmotor Symptoms of Parkinson's Disease: Antagonistic Pleiotropy Phenomena Derived from α-Synuclein Evolvability?. Parkinson's disease, 2018, 5789424. https://doi.org/10.1155/2018/5789424
  10. Lin, C. H., Yang, S. Y., Horng, H. E., Yang, C. C., Chieh, J. J., Chen, H. H., Liu, B. H., & Chiu, M. J. (2017). Plasma α-synuclein predicts cognitive decline in Parkinson's disease. Journal of neurology, neurosurgery, and psychiatry, 88(10), 818-824. https://doi.org/10.1136/jnnp-2016-314857
  11. Wang, W., Song, N., Jia, F., Tang, T., Bao, W., Zuo, C., Xie, J., & Jiang, H. (2018). Genomic DNA levels of mutant alpha-synuclein correlate with non-motor symptoms in an A53T Parkinson's disease mouse model. Neurochemistry international, 114, 71-79. https://doi.org/10.1016/j.neuint.2018.01.006
  12. Demchenko, A. V., & Biriuk, V. V. A modern view on potential biomarkers of Parkinson’s disease (review). Pathologia. 2020. 17(2), 241-247. https://doi.org/10.14739/2310-1237.2020.2.212810
  13. Yuan, Y., Tong, Q., Zhang, L., Jiang, S., Zhou, H., Zhang, R., Zhang, S., Xu, Q., Li, D., Zhou, X., Ding, J., & Zhang, K. (2016). Plasma antioxidant status and motor features in de novo Chinese Parkinson's disease patients. The International journal of neuroscience, 126(7), 641-646. https://doi.org/10.3109/00207454.2015.1054031
  14. Sarıkaya, E., & Doğan, S. (2020). Glutathione Peroxidase in Health and Diseases. In M. D. Bagatini, Glutathione System and Oxidative Stress in Health and Disease. https://doi.org/10.5772/intechopen.91009
  15. Gökçe Çokal, B., Yurtdaş, M., Keskin Güler, S., Güneş, H. N., Ataç Uçar, C., Aytaç, B., Durak, Z. E., Yoldaş, T. K., Durak, İ., & Çubukçu, H. C. (2017). Serum glutathione peroxidase, xanthine oxidase, and superoxide dismutase activities and malondialdehyde levels in patients with Parkinson's disease. Neurological sciences, 38(3), 425-431. https://doi.org/10.1007/s10072-016-2782-8
  16. Wei, Z., Li, X., Li, X., Liu, Q., & Cheng, Y. (2018). Oxidative Stress in Parkinson's Disease: A Systematic Review and Meta-Analysis. Frontiers in molecular neuroscience, 11, 236. https://doi.org/10.3389/fnmol.2018.00236
  17. Vida, C., Kobayashi, H., Garrido, A., Martínez de Toda, I., Carro, E., Molina, J., & De la Fuente, M. (2019). Lymphoproliferation Impairment and Oxidative Stress in Blood Cells from Early Parkinson’s Disease Patients. International journal of molecular sciences, 20(3), 771. https://doi.org/10.3390/ijms20030771
  18. Demchenko, A. V., Belenichev, I. F., & Bobrova, V. I. (2016). Farmakolohichna korektsiia stanu systemy hlutationu krovi khvorykh na khronichnu ishemiiu mozku [Pharmacological correction of glutathione system state in the patients with chronic cerebral ischemia]. Farmakolohiia ta likarska toksykolohiia, (6), 89-96. [in Ukrainian].
  19. Svatikova, A., Wolk, R., Wang, H. H., Otto, M. E., Bybee, K. A., Singh, R. J., & Somers, V. K. (2004). Circulating free nitrotyrosine in obstructive sleep apnea. American journal of physiology. Regulatory, integrative and comparative physiology, 287(2), R284-R287. https://doi.org/10.1152/ajpregu.00241.2004
  20. Jelic, S., Padeletti, M., Kawut, S. M., Higgins, C., Canfield, S. M., Onat, D., Colombo, P. C., Basner, R. C., Factor, P., & LeJemtel, T. H. (2008). Inflammation, oxidative stress, and repair capacity of the vascular endothelium in obstructive sleep apnea. Circulation, 117(17), 2270-2278. https://doi.org/10.1161/CIRCULATIONAHA.107.741512
  21. Cakmak, E., Yardim-Akaydin, S., Caliskan-Can, E., Firat, H., & Ardic, S. (2015). Serum nitrotyrosine and nitric oxide measurements in obstructive sleep apnea syndrome. Oxidation Communications, 38(4A), 2064-2075.
  22. Odebrecht Vargas Nunes, S., Pizzo de Castro, M. R., Ehara Watanabe, M. A., Losi Guembarovski, R., Odebrecht Vargas, H., Reiche, E. M., Kaminami Morimoto, H., Dodd, S., & Berk, M. (2014). Genetic polymorphisms in glutathione-S-transferases are associated with anxiety and mood disorders in nicotine dependence. Psychiatric genetics, 24(3), 87-93. https://doi.org/10.1097/YPG.0000000000000023
  23. Syusyuka, V. G. (2018). Kliniko-patohenetychni aspekty akusherskykh i perynatalmykh uskladnen u zhinok z urakhuvanniam psykhoemotsiinoho stanu ta yikh medyko-psykholohichna korektsiia [Clinical and pathogenetic aspects of obstetric and perinatal complications in women with regard to psycho-emotional state and their medical and psychological correction (Doctoral dissertation)]. Zaporizhzhia State Medical University, Zaporizhzhia. [in Ukrainian].

Downloads

Published

2021-08-20

How to Cite

1.
Demchenko AV, Biriuk VV, Abramov AV. The activity of markers of oxidative and nitrosative stresses in blood plasma of Parkinson’s disease patients at the early stages. Pathologia [Internet]. 2021Aug.20 [cited 2026May25];18(2):183-8. Available from: https://pat.zsmu.edu.ua/article/view/233431

Issue

Vol. 18 No. 2 (2021)

Section

Original research

License

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.

Лицензия Creative Commons