Features of angiotensin II expression in the arcuate nucleus of the hypothalamus of experimental rats with arterial hypertension of various etiology

Authors

  • S. V. Tishсhenko Zaporizhzhia State Medical University, Ukraine,

DOI:

https://doi.org/10.14739/2310-1237.2018.3.151864

Keywords:

hypothalamus, arcuate nucleus, angiotensin II, arterial hypertension, rats

Abstract

It is proved that the effctiveness of blood pressure regulation mainly depends on those neuromodulators and neurohormones
that are formed in or enter the hypothalamus. The important role of the arcuate nucleus (ArC) both as the intrahypothalamic
regulator of the functional activity of its nuclear structures, and the coordinator over the suprasegmental and segmental centers of blood pressure regulation is proved. There are data that blood pressure is dependent on the content of the pressor neurohormone angiotensin II in the ArC of hypothalamus.
The aim of the study was to determine the specifi properties of angiotensin II content in the ArC of hypothalamus in the rats of Wistar and SHR lines with etiologically diffrent arterial hypertension: endocrine-salt and essential.
Materials and methods. The study was carried out on 24 mature male rats 250–270 g weight at the age of 13–14 months, which were divided into three experimental groups each consisting of 8 animals. First group of the Wistar rats with normal BP parameters (Ps/Pd = 110/75 ± 5 mm) was a control one. The second was the group of Wistar rats with the endocrine-salt model
of arterial hypertension (Ps/Pd 145/110 ± 10 mm). The third group was presented by the SHR rats with spontaneous arterial hypertension (Ps/Pd = 150/110 ± 10 mm). An immunohistochemical method was used to study the content of angiotensin II in the ArC, which was followed by digital processing of the data by Image J and EXCEL-7.0.
Results. Reduction in the content and concentration of angiotensin II is observed in the rats with arterial hypertension independently of its etiology and pathogenesis. Peculiarities of angiotensin II content in ArC of hypothalamus are dependent from the etiology and pathogenesis of arterial hypertension. In essential hypertension (SHR) lower levels of angiotensin II expression in the ArC structure of the hypothalamus are observed than in secondary endocrine associated arterial hypertension.

References

Mozaffarian, D., & Benjamin, E. J. (2015). Heart disease and stroke statistics-(2015) update: a report from the American Heart Association. Circulation, 4, 29–322.

Gerasimova, A. S., & Olejnikov, V. E. (2008). Arterial'naya gipertenziya associirovannaya s metabolicheskim sindromom, osobennosti porazheniya organov mishenej. Obzor literatury [Arterial hypertension associated with metabolic syndrome: features of the course and lesion of target organs. Literature Reviews]. Medicinskie nauki, 3. [in Russian].

Kawabe, T., Kawabe, K., & Sapru, H. (2012). Cardiovascular Responses to Chemical Stimulation of the Hypothalamic Arcuate Nucleus in the Rat: Role of the Hypothalamic Paraventricular Nucleus. PLoS ONE, 7(9), e45180. doi: 10.1371/journal.pone.0045180.

Chronwall, B. (1985). Anatomy and physiology of the neuroendocrine arcuate nucleus. Peptides, 6, 1–11. doi: 1016/0196-9781(85)90128-7.

Peruzzo, B., Pastor, F. E, Blazquez, J. L., Amat, P., & Rodríguez, E. M. (2004). Polarized endocytosis and transcytosis in the hypothalamic tanycytes of the rat. Cell Tissue Res. 317(2), 147–164. doi: 10.1007/s00441-004-0899-1.

Arakawa, H., Chitravanshi, V., & Sapru, H. (2011). The hypothalamic arcuate nucleus: a new site of cardiovascular action of angiotensin-(1–12) and angiotensin II. American Journal of Physiology-Heart and Circulatory Physiology, 300(3), H951–960. doi: 10.1152/ajpheart.01144.2010.

Iellamo, F. (2001). Neural mechanisms of cardiovascular regulation during exercise. Autonomic Neuroscience, 90(1–2), 66–75. doi: 10.1016/S1566-0702(01)00269-7.

McKinley, M. J., Albiston, A. L., Allen, A. M., Mathai, M. L., May, C. N., McAllen, R. M., et al. (2003). The brain renin-angiotensin system: location and physiological roles. The International Journal of Biochemistry & Cell Biology, 35(6), 901–918. doi.org/10.1016/S1357-2725(02)00306-0.

Norstrom, A., Hansson, H., & Sjostrand, J. (1971). Effects of colchicine on axonal transport and ultrastructure of the hypothalamo-neurohypophyseal system of the rat. Zeitschrift for Zellforschung Und Mikroskopische Anatomie, 113(2), 271–293. doi.org/10.1007/BF00339421.

Paxinos, G., & Watson, C. (2007). The Rat Brain in Stereotaxic Coordinates. Academic Press.

Zajcev, V., Liflyandskij, V., & Marinkin, V. (2003) Prikladnaya medicinskaya statistika [Applied medical statistics]. Saint Petersburg: Foliant. [in Russian].

Nazarali, A. J., Gutkind, J. S., Correa, F. M., & Saavedra, J. M. (1990). Decreased Angiotensin II Receptors in Subfornical Organ of Spontaneously Hypertensive Rats After Chronic Antihypertensive Treatment With Enalapril. American Journal of Hypertension, 3(1), 59–61. doi: 10.1093/ajh/3.1.59.

Gunther, S., & Gimbrone M. A., & Alexander, R. W. (1980) Regulation by angiotensin II of its receptors in resistance blood vessels. Nature, 287, 230–232. doi: 10.1038/287230a0.

Eudy, R. J., Sahasrabudhe, V., Sweeney, K., Tugnait, M., King-Ahmad, A., Near, K. et al. (2011). The use of plasma aldosterone and urinary sodium to potassium ratio as translatable quantitative biomarkers of mineralocorticoid receptor antagonism. Journal Of Translational Medicine, 9, 180. doi: 10.1186/1479-5876-9-180.

How to Cite

1.
Tishсhenko SV. Features of angiotensin II expression in the arcuate nucleus of the hypothalamus of experimental rats with arterial hypertension of various etiology. Pathologia [Internet]. 2018Dec.19 [cited 2024Nov.13];(3). Available from: http://pat.zsmu.edu.ua/article/view/151864

Issue

Section

Original research