Histological and immunohistochemical features of the ovarian malignant epithelial tumors and granulosa cell tumors

Authors

  • O. A. Savchenko SE «Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine», Dnipro,
  • I. S. Shponka SE «Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine», Honored Worker of Science and Technology of Ukraine, Dnipro,
  • V. R. Skoryk SE «Dnipropetrovsk Medical Academy of the Ministry of Health of Ukraine», Dnipro,
  • P. V. Savchenko SE «Dnipropetrovsk medical academy of the Ministry of Health of Ukraine», Dnipro,

DOI:

https://doi.org/10.14739/2310-1237.2019.2.177078

Keywords:

ovarian cancer, granulosa cell tumors, histological type of neoplasm, immunohistochemistry

Abstract

 

Among cancers the ovarian cancer is one of the top ten in prevalence occupying the 7th place and one of the first five in mortality occupying the 4th place. These facts are explained, on the one hand, by the absence of specific clinical manifestations, which causes the diagnosis of the process at its later stages with a frequent metastatic lesion. On the other hand, there is a problem of reliable verification of the diagnosis.

The purpose of the study is to improve the complex of differential diagnostic morphological criteria for malignant neoplasms of the ovaries verification, which is based on the latest international classifications and features of practical oncomorphology in Ukraine.

Methods. Retrospective analysis of 115 malignant ovarian tumors (where 67 cases of different types carcinomas and 48 cases of granulosa cell tumor were included) contained assessment of histological, immunohistochemical characteristics followed by statistical processing of the results.

Results. Received data made it possible to state the diagnostic value of each feature for the studied malignant tumors of the ovaries. Among the histological characteristics the most important were: macro- and micropapillas, follicular / glandular structures, polymorphism degree, mitotic activity, cell necrosis. Among the panel of immunohistochemical antibodies the fractions of cytokeratins (pancytoceratin AE1 / AE3, cytokeratin 7, cytokeratin 20), p53, WT-1, EMA and vimentin were mentioned. However, P > 0.05 for all the indicators.

Conclusions. The necessity of using is based on a combination of histological criteria for a specific panel of IgG antibodies, which is statistically confirmed. Presence of papillary structures, mitotic activity, polymorphism and expression of p53 are clue for differentiation between HGSC and LGSC. Granulosa cell tumors had a special phenotype Vimentin + / Calretinin + / PanCK + (focally).

 

References

Fedorenko et al. (2019) Biuleten natsionalnoho kantser-reiestru Ukrainy №20 – «Rak v Ukraini, 2017–2018» [Bulletin of the National Chancery Register of Ukraine No.20. "Cancer in Ukraine 2017–2018"]. (P. 52–53). Kyiv. [in Ukrainian].

Pozharisskiĭ, K. M., Raskin, G. A., Vinokurov, V. L., Iurkova, L. E., & Mukhina, M. S. (2015). Immunogistikhimicheskie osobennosti kletok seroznoi adenokarzinomy yaichnikov, opredeliaiushchie klinicheskoe techenie zabolevaniia i vyzhivaemost' bol'nykh [The immunohistochemical features of ovarian serous adenocarcinoma cells, which determine a disease course and survival rates in patients]. Arhiv patologii, 77(1), 38–40. [in Russian]. doi: 10.17116/patol201577138-

Savshenko, O. A. & Shponka, І. S. (2018). Analis rivnia ekspresii pantsytokeratyniv v epitelialnykh ta hranulosoklitynnykh pukhlynakh yaiechnykiv [Analysis of the expression of pancitotereperatesinepithelialandgranulosicovariantumorcells.]. Medychni perspektyvy, 23(2). [in Ukrainian]. doi: https://doi.org/10.26641/2307-0404.2018.2.133693

Kitamura, S., Abiko, K., Matsumura, N., Nakai, H., Akimoto, Y., Tanimoto, H., & Konishi, I. (2017). Adult granulosa cell tumors of the ovary: a retrospective study of 30 cases with respect to the expression of steroid synthesis enzymes. Journal of gynecologic oncology, 28(4). e31. doi: 10.3802/jgo.2017.28.e31

Kucukodaci, Z., Keles, M., Yapanoglu, T., Alp, B. F., Aksoy, Y., & Ozbey, I. (2008). Adult type granulosa cell tumor of the testis: case report and review of the literature. The Eurasian journal of medicine, 40(1), 39–41.

Bennett, J. A., Dong, F., Young, R. H., & Oliva, E. (2015). Clear cell carcinoma of the ovary: evaluation of prognostic parameters based on a clinicopathological analysis of 100 cases. Histopathology, 66(6), 808–815. doi: 10.1111/his.12514

Fletcher, C. D. M. (2013). Diagnostic histopatology of tumors.

Ahn, G., Folkins, A. K., McKenney, J. K., & Longacre, T. A. (2016). Low-grade Serous Carcinoma of the Ovary. The American journal of surgical pathology, 40(9), 1165–1176. doi: 10.1097/PAS.0000000000000693

Okoye, E., Euscher, E. D., & Malpica, A. (2016). Ovarian Low-grade Serous Carcinoma. The American journal of surgical pathology, 40(5), 627–635. doi: 10.1097/PAS.0000000000000615

Miyamoto, M., Takano, M., Aoyama, T., Soyama, H., Yoshikawa, T., Tsuda, H., & Furuya, K. (2017). Seromucinous component in endometrioid endometrial carcinoma as a histological predictor of prognosis. Journal of gynecologic oncology, 29(2), e20. doi: 10.3802/jgo.2018.29.e20

Anglesio, M. S., Wang, Y. K., Maassen, M., Horlings, H. M., Bashashati, A., Senz, J., et al. (2016). Synchronous endometrial and ovarian carcinomas: evidence of clonality. JNCI: Journal of the National Cancer Institute, 108(6). djv428. doi: 10.1093/jnci/djv428

McNamee, T., Damato, S., & McCluggage, W. G. (2016). Yolk sac tumours of the female genital tract in older adults derive commonly from somatic epithelial neoplasms: somatically derived yolk sac tumours. Histopathology, 69(5), 739–751. doi: 10.1111/his.13021

Li, J., Bao, R., Peng, S., & Zhang, C. (2018). The molecular mechanism of ovarian granulosa cell tumors. Journal of ovarian research, 11(1), 13. doi: 10.1186/s13048-018-0384-1

Kurman, R. J., Carcangiu, M. L., Herrington, S., & Young, R. H.

(2014). WHO classification of tumours of female reproductive organs. Lyon: IARC.

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How to Cite

1.
Savchenko OA, Shponka IS, Skoryk VR, Savchenko PV. Histological and immunohistochemical features of the ovarian malignant epithelial tumors and granulosa cell tumors. Pathologia [Internet]. 2019Sep.2 [cited 2024Nov.15];(2). Available from: http://pat.zsmu.edu.ua/article/view/177078

Issue

No. 2 (2019)

Section

Original research

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