Immunohistochemical characteristics of the angiogenesis processes in brain tissue in diabetes mellitus type 2
DOI:
https://doi.org/10.14739/2310-1237.2020.1.203736Keywords:
diabetes mellitus, angiogenesis, VEGF, VEGFR-2, CD31, CD105Abstract
Aim – to study the features of VEGF, VEGFR-2 and CD31, CD105 immunohistochemical expression in the cerebral cortex in diabetes mellitus type 2.
Materials and methods. Pathomorphological and immunohistochemical studies of the brain tissue section material were performed in 3 groups of observations: group I – control without DM and cerebrovascular pathology, group II – died with dyscirculatory-ischemic encephalopathy (DIE), group III – died with DM type 2.
Results. Increased expression of VEGF and VEGFR-2 was found both in dyscirculatory-ischemic encephalopathy and in patients with diabetes mellitus type 2 (VEGF in the control group – 0.337 (0.232; 0.617) %, in the group with DIE – 0.713 (0.438); 1.304) %, in the group with diabetes mellitus type 2 – 1.003 (0.699; 1.631) %; VEGFR-2 in the group of control – 0.916 (0.550; 1.56) %, in the group with DIE – 1,238 (0.76; 1.61) %, in the group with DM type 2 – 1.15 (0.58; 1.784) %. There is direct correlation between the level of expression of CD105 and VEGFR-2: r = 0.31 for diabetes type 2. The distribution density of microvessels significantly increases in the group with DM type 2. Significant increase in expression of CD105 in the group with DM type 2 (0.434 (0.265; 0.741) %) was found, compared with the group of control (0.346 (0.263; 0.46) %). There are direct correlations between CD31 and CD105 expression in DIE (r = 0,41) and in the group with DM type 2 (r = 0,39, P < 0.05).
Conclusions. Angiogenesis is activated in the cerebral cortex in diabetes type 2, which may be due to the activation of the transcription factor HIF-1a.
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