Peri-implant osteogenesis on alumina-coated titanium implants in rat femur: morphological and elemental analysis of implant surfaces
DOI:
https://doi.org/10.14739/2310-1237.2024.2.306822Keywords:
orthopedics, implants, functional-protective coatings, corundum ceramics, aluminum oxide, osteoinduction, osseointegration, scanning electron microscopy, energy dispersive X-ray spectroscopy, peri-implant osteogenesisAbstract
Peri-implant bone tissue regeneration involves complex processes that are not yet fully understood at the cellular and molecular levels, leaving significant gaps in our knowledge that require further investigation.
Aim. The study aimed to compare peri-implant osteogenesis on titanium femoral implants with alumina composite coatings applied by different methods to conventional titanium implants in an animal model.
Materials and methods. Implants underwent sandblasting with silicon carbide, plasma torch treatment, and coating with titanium, corundum, sprayed titanium wire, or hydroxyapatite, resulting in seven different surfaces. 105 female Wistar rats received implants in their right femurs and were divided into 7 groups based on implant type and exposure duration (1, 2, or 4 weeks). Implant fragments were analyzed using scanning electron microscopy and energy dispersive X-ray spectroscopy to quantify chemical elements. Ratios of carbon to nitrogen and calcium to phosphorus were calculated. Data were analyzed using the U-Mann–Whitney test, with р < 0.05 as a significant value.
Results. The energy dispersive X-spectrometry results confirmed morphological analysis findings by quantitatively and qualitatively assessing implants surface chemical composition. The key elements were evaluated, relevant for identifying bone tissue components like collagen (C and N) and hydroxyapatite (Ca and P), as well as implant coatings (Ti, Al, Ca, and P). Carbon and phosphorus showed fluctuations over time, with notable differences among groups. Aluminum appeared stable in some groups but varied in others. Calcium levels remained low initially and increased steadily in hydroxyapatite coated implants. Titanium levels were high initially, decreasing slightly over time. Morphological analysis correlated with surface roughness measurements. Notably, fibrin, collagen, and bone tissue presence varied among groups over time, with some groups showing significant mineralized bone tissue accumulation. After four weeks, blood clots persisted in some groups, while others exhibited bone tissue remodeling with the presence of osteoblasts and osteoclasts. Alumina-based coatings showed signs of degradation, with alumina cement scales found among macrophages and fibers.
Conclusions. Our study found that stable bone implants outperform alumina-composite coatings in long-term osseointegration due to mechanical stability. Although ceramic composites initially enhance osteoinductive properties, better attachment to titanium substrates is needed.
References
Kumar M, Kumar R, Kumar S. Coatings on orthopedic implants to overcome present problems and challenges: A focused review. Materials Today: Proceedings. 2021;45(6):5269-76. doi: https://doi.org/https://doi.org/10.1016/j.matpr.2021.01.831
Huynh V, Ngo NK, Golden TD. Surface Activation and Pretreatments for Biocompatible Metals and Alloys Used in Biomedical Applications. Int J Biomater. 2019;2019:3806504. doi: https://doi.org/https://doi.org/10.1155/2019/3806504
Zhang LC, Chen LY. A review on biomedical titanium alloys: recent progress and prospect. Adv Eng Mater. 2019;21(4):1801215. doi: https://doi.org/https://doi.org/10.1002/adem.201801215
Schüpbach P, Glauser R, Rocci A, Martignoni M, Sennerby L, Lundgren A, et al. The human bone-oxidized titanium implant interface: A light microscopic, scanning electron microscopic, back-scatter scanning electron microscopic, and energy-dispersive x-ray study of clinically retrieved dental implants. Clin Implant Dent Relat Res. 2005;7 Suppl 1:S36-S43. doi: https://doi.org/https://doi.org/10.1111/j.1708-8208.2005.tb00073.x
Bahraminasab M, Arab S, Safari M, Talebi A, Kavakebian F, Doostmohammadi N. In vivo performance of Al2O3-Ti bone implants in the rat femur. J Orthop Surg Res. 2021;16(1):79. doi: https://doi.org/https://doi.org/10.1186/s13018-021-02226-7
Albrektsson T, Johansson C. Osteoinduction, osteoconduction and osseointegration. Eur Spine J. 2001;10 Suppl 2:S96-101.doi: https://doi.org/https://doi.org/10.1007/s005860100282
Davies JE. Bone bonding at natural and biomaterial surfaces. Biomaterials. 2007;28(34):5058-67. doi: https://doi.org/https://doi.org/10.1016/j.biomaterials.2007.07.049
Iancu IA, Iancu SA, Epistatu D, Comaneanu M, Badarau IA. Scanning electron microscopy and energy-dispersive X-ray spectroscopy on the degree of bone mineralization at the bone-implant interface. Ro J Stomatol. 2024;70(1):21-6. doi: https://doi.org/https://doi.org/10.37897/RJS.2024.1.7
Shah FA, Ruscsák K, Palmquist A. 50 years of scanning electron microscopy of bone-a comprehensive overview of the important discoveries made and insights gained into bone material properties in health, disease, and taphonomy. Bone Res. 2019;7:15. doi: https://doi.org/https://doi.org/10.1038/s41413-019-0053-z
Mangano F, Raspanti M, Maghaireh H, Mangano C. Scanning Electron Microscope (SEM) Evaluation of the Interface between a Nanostructured Calcium-Incorporated Dental Implant Surface and the Human Bone. Materials (Basel). 2017;10(12):1438. doi: https://doi.org/https://doi.org/10.3390/ma10121438
Depprich R, Zipprich H, Ommerborn M, Mahn E, Lammers L, Handschel J, et al. Osseointegration of zirconia implants: an SEM observation of the bone-implant interface. Head Face Med. 2008;4:25. Published 2008 Nov 6. doi: https://doi.org/https://doi.org/10.1186/1746-160X-4-25
Loskutov O, Shponka I, Bondarenko O, Bondarenko N, Bozhko A. Histological and histochemical assessment of short-term events in peri-implant bone for osteoinductivity evaluation of functional-protective implant coatings. Medicni perspektivi. 2021;26(3):4-10. doi: https://doi.org/https://doi.org/10.26641/2307-0404.2021.3.241875
Schwarcz HP, Nahal H. Theoretical and observed C/N ratios in human bone collagen. J Archeol Sci. 2021;131:105396.doi: https://doi.org/https://doi.org/10.1016/j.jas.2021.105396
Hernandez-Becerra E, Londoño-Restrepo SM, Hernández-Urbiola MI, Jimenez-Mendoza D, Aguilera-Barreiro ML, Perez-Torrero E, et al. Determination of basal bone mineral density in the femur bones of male and female Wistar rats. Lab Anim. 2021;55(1):30-42. doi: https://doi.org/https://doi.org/10.1177/0023677220922566
Nikolova MP, Apostolova MD. Advances in Multifunctional Bioactive Coatings for Metallic Bone Implants. Materials (Basel). 2022;16(1):183. doi: https://doi.org/https://doi.org/10.3390/ma16010183
Büchter A, Joos U, Wiesmann HP, Seper L, Meyer U. Biological and biomechanical evaluation of interface reaction at conical screw-type implants. Head Face Med. 2006;2:5. doi: https://doi.org/https://doi.org/10.1186/1746-160X-2-5
Pobloth AM, Mersiowsky MJ, Kliemt L, Schell H, Dienelt A, Pfitzner BM, et al. Bioactive coating of zirconia toughened alumina ceramic implants improves cancellous osseointegration. Sci Rep. 2019;9(1):16692. doi: https://doi.org/https://doi.org/10.1038/s41598-019-53094-5
Ibrahim SW, Rafeeq AK, Ahmedhamdi MS. Histomorphometric assessment of implant coated with mixture of nano-alumina and fluorapatite in rabbits. Saudi Dent J. 2021;33(8):1142-8. doi: https://doi.org/https://doi.org/10.1016/j.sdentj.2021.02.005
Lin S, Maekawa H, Moeinzadeh S, Lui E, Alizadeh HV, Li J, et al. An osteoinductive and biodegradable intramedullary implant accelerates bone healing and mitigates complications of bone transport in male rats. Nat Commun. 2023;14(1):4455. doi: https://doi.org/https://doi.org/10.1038/s41467-023-40149-5
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