Histopathological features and protein ubiquitin immunohistochemical expression in peritumoral tissue of the testicular germ cell tumors

Authors

  • S. V. Bazalytska
  • A. M. Romanenko

DOI:

https://doi.org/10.14739/2310-1237.2015.1.42827

Keywords:

Ovarian Neoplasms, Seminiferous Tubules, Proteins, TIN

Abstract

Aim. For the purpose of determination of characteristic morphological changes as well as the immunohistochemical protein Ubiquitin expression in peritumoral testicular tissue, which could be characterized as precancer lesions, the 40 patients with testicular germ cell tumors (TGCT) were investigated.

Methods and results. Peritumoral testicular tissue in patients with the spermatogenesis alteration (that was detected in 95% of cases) demonstrated: 1) the dramatic increase of ubiquitination processes in seminiferous tubules, confirming the intensive proteolysis of  the damaged intracellular proteins, 2) occurrence of atypical germ cells (TIN), which differ from the normal spermatogenesis cells by the authentically lower levels of the nuclear and cytoplasmatic protein Ubiquitin expression, 3) alteration of ubiquitination processes in Leydig cells with the significant increase of the protein Ubiquitin cytoplasmatic expression and the total disappearance of protein Ubiquitin nuclear expression.

Conclusion. Our results show the important role of the structural and functional ubiquitin-proteolysis system components alterations at the initial stages of the testicular tissue carcinogenesis.

References

(2013) Rak v Ukraini, 2011–2012. Zakhvoriuvanist, smertnist, pokaznyky diialnosti onkolohichnoi sluzhby. [Cancer in Ukraine, 2011–2012. Disease, mortality, indicators of activity oncologic service]. Biuleten Natsionalnoho kantser-reiestru Ukrainy, 14. [in Ukrainian].

Ciechanover, A. (1998). The ubiquitin-proteasome pathway: on protein death and cell life. EMBO J., 17(24), 7151–7160. doi: 10.1093/emboj/17.24.7151.

Ciechanover, A. (2003). The ubiquitin proteolytic system and pathogenesis of human diseases: a novel platform for mechanism-based drug targeting. Biochem. Soc. Trans., 31(2), 474–481. :doi: 10.1042/BST0310474.

Huyghe, E., Soulie, M., Escourrou, G., Mieusset, R., Plante, P., & Thonneau,P. (2005). Conservative management of small testicular tumors relative to carcinoma in situ prevalence. J. Urol., 173, 820–823. doi: 10.1097/01.ju.0000152532.34475.4e.

Ng, J. M., Vrieling, H., Sugasawa, K., Ooms, M. P., Grootegoed, J. A, Vreeburg, J. T., et al. (2002). Developmental defects and male sterility in mice lacking the ubiquitin-like DNA repair gene mHR23B. Mol. Cell. Biol., 22(4), 1233–1245. doi: 10.1128/MCB.22.4.1233-1245.2002.

Malmstrum, P. U., Busch, C., Norben, B. J., & Andersson, B. (1998). Expression of ABH blood group isoantigen as a prognostic factor in transitional cell bladder carcinoma. Scand. J. Urol. Nephrol., 22, 265–270.

Mostofi, F. K., Sesterhenn, I. A., & Sobin, L. H. (1998). Histological typing of testis tumours. Berlin.

Tal, R., Holland, R., Belenky, A., Konichezky, M., & Baniel, J. (2004) Incidental testicular tumors in infertile men. Fertil. Steril., 82, 469–471. doi: 10.1016/j.fertnstert.2003.12.048.

Raman Jay, D., Nobert Craig, F., & Goldstein, M. (2005) Increased incidence of testicular cancer in men presenting with infertility and abnormal semen analysis. The Journal of Urology, 174, 1819–1822.

Bedard, N., Hingamp, P., Pang, Z., Karaplis, A., Morales, C., Trasler, J., et al. (2005). Mice lacking the UBC4-testis gene have a delay in postnatal testis development but normal spermatogenesis and fertility. Mol. Cell. Biol., 25(15), 6346–6354. doi: 10.1128/MCB.25.15.6346-6354.2005.

Linke, J., Loy, V., & Dieckmann, K. P. (2005). Prevalence of testicular intraepithelial neoplasia in healthy males. J. Urol, 173, 1577–1579. doi: 10.1097/01.ju.0000154348.68575.95.

Jonnes, T. D., MacLennan, G. T., Bonnin, J. M., Varsegi, M. F., Biair, J. E., Cheng, L. (2006). Screening for intratubular neoplasia of the testis using OCT4 immunoihistochemistry. Am. J. Surg. Pathology, 30(11), 1427–1431. doi: 10.1097/01.pas.0000213288.50660.f7.

Stoop, H., Honecker, F., van de Geijn, G. J., Gillis, A. J., Cools, M. C., de Boer, M., et al. (2008). Stem cell factor as a novel diagnostic marker for early malignant germ cells. J. Pathology, 216(1), 43–54. doi: 10.1002/path.2378.

Sutovsky, P. (2003). Ubiquitin-dependent proteolysis in mammalian spermatogenesis, fertilization, and sperm quality control: killing three birds with one stone. Microsc. Res. Tech., 61(1), 88–102. doi: 10.1002/jemt.10319.

Glickman, M. H., & Ciechanover, A. (2002). The ubiquitin-proteasome proteolytic pathway: destruction for the sake of construction. Physiol. Rev., 82, 373–428. doi: 10.1152/physrev.00027.2001.

How to Cite

1.
Bazalytska SV, Romanenko AM. Histopathological features and protein ubiquitin immunohistochemical expression in peritumoral tissue of the testicular germ cell tumors. Pathologia [Internet]. 2015May19 [cited 2024Nov.24];(1). Available from: http://pat.zsmu.edu.ua/article/view/42827

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Section

Original research