Complex pathologic differential diagnosis of precancerous processes and gastric cancer

Authors

  • V.D. Markovskiy
  • A.V. Kharchenko

DOI:

https://doi.org/10.14739/2310-1237.2012.3.16987

Keywords:

dysplasia, phenotypes, amplicones, DNA, PCR

Abstract

For the first time, a special comprehensive study of the phenotypes of dysplasia of gastric mucosa by histological techniques and their respective genomes using  molecular-biological   method of ISSR-PCR was performed.    Main  effect of precancerous lesions, chronic atrophic gastritis and  Helicobacter pylori infection in the formation of precancerous changes in the form of gastric epithelium dysplasia and in the form of intestinal metaplasia foci was shown.  We study manifestations of the mitotic regime in various zones of gastric mucosa in patients with chronic duodenal ulcer, gastric ulcer, and ulcero-infiltrative gastric cancer. The regularities and characteristics of the frequency and prevalence of precancerous processes in different zones of stomach in patients with duodenal ulcer and stomach ulcer were determined. Diagnostic criteria for differenting them from stomach cancer were worked out. On the material of postoperative stomach gastrobiopsies repeated during five years, statement about heterogeneity of gastric mucosa epithelium dysplasia of the second and third degree was grounded, and criteria for the detection of early gastric cancer using histological methods in combination with the method of ISSR-PCR were found. The use of ISSR-PCR method for detecting  tumor cells in peripheral blood of patients with category М0   cancer revealed their presence  in 27,8% cases, which indicates risk of early metastasis in these patients.

References

Абрамов Д.Д. Точность метода полимеразной цепной реакции «в реальном времени / Абрамов Д.Д., Трофимов Д.Ю., Ребриков Д.В. // Прикл. биохимия и микробиология. – 2006. – №42. – С. 485–488.

Алов И.А. Определение митотического режима ткани в патогистологической диагностике предраковых процессов и рака: метод. указания) / Алов И.А., Аспиз М.Е., Казанцева И.А. – М., 1973. – 143 с.

Канцерогенез / Под ред Д.Г.Заридзе. – М.: Медицина, 2004. – 576 с.

ПЦР «в реальном времени» / [Ребриков Д.В., Саматов Г.А., Трофимов Д.Ю., Семенов П.А., Савилова А.М., Кофиади И.А., Абрамов Д.Д.]; под ред. Д.В. Ребрикова. – М.: БИНОМ. Лаборатория знаний, 2009. – 215 с.

Садчиков В.Д. Дисплазия покровно-ямочного эпителия слизистой оболочки желудка / Садчиков В.Д. // Врачебное дело. – 1986. – №10. –С. 65–68.

Свердлов Е.Д. Перспективы использования достижений геномики в медицине: начало эры полногеномной медицинской генетики / Свердлов Е.Д. // Журн. патол. физиол. эксперим. терапии. – 2001. – №1. – С. 3–22.

Mullis K.B. Specific syntesis of DNA in vitro via a polymerase catalyzed chain reaction / Mullis K.B. and Faloona F. // Meth. Ensymol. – 1987. – №155. – C. 335–350.

Nagayo T. Dysplasia of the gastric mucosa and its relation to the precancerous state / Nagayo T. // GANN. – 1981. – Vol. 72, №6. – P. 813–823.

Tsanev R. Molecular mechanisms of cancer cells survival / Tsanev R. // J.BUON. – 2005. – №10. – P. 309–318.

How to Cite

1.
Markovskiy V, Kharchenko A. Complex pathologic differential diagnosis of precancerous processes and gastric cancer. Pathologia [Internet]. 2013Aug.29 [cited 2024Nov.13];(3). Available from: http://pat.zsmu.edu.ua/article/view/16987

Issue

Section

Original research