Estimation of thiocetam influence on glutathione link of thiol disulfide cerebral system in conditions of experimental chronic ischemia

Authors

  • A. V. Demchenko
  • І. F. Belenichev

DOI:

https://doi.org/10.14739/2310-1237.2015.2.51154

Keywords:

Brain ischemia, Tiocetam

Abstract

Aim. Pharmacological remodeling of pathologic changes when cerebral ischemia takes place is actual task for today. With the purpose of thiocetam neuroprotective properties studying in conditions of experimental chronic cerebral ischemia the investigations of 45 white rats with simulated chronic cerebral ischemia have been carried out.


Methods and results. A number of biochemical, enzyme immunoassay, pharmacological and statistic methods of investigation were used. Positive thiocetam influence on molecular and biochemical changes in brain of laboratory animals subjected to ischemia is established. This influence is showed in decreasing processes of oxidative and nitrosative stresses: increasing of reduced glutathione contents, activity of antioxidant enzymes of glutathione link, recreation of thiol disulfide state of a cell, decreasing of neurotoxic compound contents of nitrotyrosine in cerebral tissue.


Conclusion. A positive influence of thiocetam on remembering processes among laboratory animals with simulated chronic cerebral ischemia has been noted.

 

References

Arushanyan, E. B., & Beier, E. V. (2007). Gippokamp i narusheniya poznavatel'noj deyatel'nosti [Hyppocampus and cognitive disturbances]. Zhurnal nevrologii i psikhiatrii, 7, 72–77. [in Russian].

Belenichev, I. F. Chernij, V. I., & Kolesnyk, Yu. M. (2009). Racional`naya nejroprotekciya [Rational neuroprotection]. Doneck: Zaslavskij. [in Ukrainian].

Buresh, Ya., Bureshova, O., & H'yuston, D. (1991). Metodiki i osnovnye e`ksperimenty po izucheniyu mozga i povedeniya [Methods and principle experiments of studing brain and behaviour] Moscow: Vysshaya shkola. [in Russian].

Vasylieva, N. V., Bilous, I. I., Yaremchuk, O. B., & Zhukovskyi, O. O. (2011). Otsinka efektyvnosti tiotsetamu u khvorykh na dystsyrkuliatornu entsefalopatiiu [Estimation of efficiency of thiocetam in patients with dyscirculatory encephalopathy]. Visnyk naukovykh doslidzhen, 4, 138–139. [in Ukrainian].

Gubskij, Yu. I., Belenichev, I. F., & Pavlov, S. V. (2005). Toksikologicheskie posledstviya okislitel'noj modifikacii belkov pri razlichnykh patologicheskikh sostoyaniyakh (obzor literatury) [Toxicological consequences of oxidative midification of proteins in different pathological states (survey of literature)]. Sovremennye problemy toksikologii, 3, 20–26. [in Ukrainian].

Dosenko, V. Ye., Zahorii, V. Yu., & Moibenko, O. O. (2002). Patofiziolohichni aspekty henetychnoho polimorfizmu endotelialnoi NO-syntazy [Pathophysiological aspects of endothelial NO-synthase genetic polymorphism]. Fiziolohichnyi zhurnal, 48(6), 86–101. [in Ukrainian].

Kozelkin, A. A., Kozelkina, S. A., & Cherkez, A. N. (2006). Dinamika kognitivnykh rasstrojstv u bol'nykh s khronicheskoj nedostatochnost'yu mozkovogo krovoobrascheniya na fone kompleksnogo lecheniya s primeneniem preparata Tiocetam [Dynamics of cognitive diseases among patients with chronical cerebral circulatory insufficiency against the background of holiatry treatment using Tiocetam]. Mezhdunarodnyj nevrologicheskij zhurnal, 5, 57–60. [in Ukrainian].

Chekman, I. S., Gubskij, Yu. I., & Belenichev, I. F. (2010) Doklinicheskoe izuchenie specificheskoj aktivnosti potencial'nykh nejroprotektivnykh preparatov [Pre-clinical studying of potential neuroprotecor medicines specific activity]. Кyiv: GFC MZ Ukrainy. [in Ukrainian].

Di Matteo, M. A., Loweth, A. C., Thomas, S., Mabley, J. G., Morgan, N. G., Thorpe, J. R., & Green, I. C. (1997). Superoxide, nitric oxide, peroxynitrite and cytokine combinations all cause functional impairment and morpfological changes in rat islets of Langerhans and insulin secreting cell lines, but dictate cell death by different mechanisms. Apoptosis, 2, 164–169. doi: 10.1023/A:1026412414666.

Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265–275.

Downloads

How to Cite

1.
Demchenko AV, Belenichev ІF. Estimation of thiocetam influence on glutathione link of thiol disulfide cerebral system in conditions of experimental chronic ischemia. Pathologia [Internet]. 2015Oct.8 [cited 2024Nov.8];(2). Available from: http://pat.zsmu.edu.ua/article/view/51154

Issue

No. 2 (2015)

Section

Original research

License

Authors who publish with this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Лицензия Creative Commons
    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.

  1. Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeThe Effect of Open Access).