Pathomorphology of rat brain meninges in experimental subarachnoid hemorrhage

Authors

  • A. M. Netlyukh Danylo Halitsky Lviv National Medical University,

DOI:

https://doi.org/10.14739/2310-1237.2015.3.56110

Keywords:

Intracranial Aneurysm, Subarachnoid Hemorrhage, Brain, Nitric Oxide Donors

Abstract

Aim. To study pathomorphological changes and vascular reactions of meninges and influence of nitric oxide donators on it in subarachnoid hemorrhage.

Methods and results. Eighteen white rats underwent suboccipital injection of autologous arterial blood to create model of subarachnoid hemorrhage (SAH) and study microanatomy of brain meninges stained with hematoxylin-eosin and metylen blue-basic fuchsine. Disorientation of dural collagen microfibers, infiltration with leukocytes, irregularity of connective fibers structure and tissue cell apoptosis were noted. In animals with SAH after treatment with donor of nitric oxide L-arginin Tivortin on background of arteriolar smooth muscle layer edema order of myocytes nuclei in accordance with their spiral orientation in thickened media was noted, spindly nuclei of endothelial cells are normochromic, located evenly along basal membranes of arterioles, apoptosis phenomenon in micropreparations does not occur.

Conclusion. This shows that nitric oxide donator L-arginin injections provide reduction of inflammatory, necrobiotic processes and cell apoptosis of brain membranes and arterioles in the experimental SAH.

References

Shvedskyi, V. V., Shtryhol, S. Yu., & Khodakivskyi, О. А. (2011). Suchasna tserebroprotektorna terapiia hostrykh porushen mozkovoho krovoobihu pry tsukrovomu diabeti ta shliakhy yii optymizatsii [Modern cerebroprotective therapy of acute brain circulation disorders in diabetes mellitus and ways of its optimisation]. Klinichna farmatsiia, 15(2), 7–12. [in Ukrainian].

Bahauri, O. V., & Khodakivskyi, O. A. (2013). Kharakterystyka morfolohichnykh zmin somato-sensornoi kory holovnoho mozku shchuriv na tli eksperymentalnoi terapii modelnoho insultu pokhidnym 3,2-spiro-pirrolo-2-oksindolu (spoluka r-86) ta tsytykolinom [Characteristics of Morphological Changes of Somatosensory Cerebral Cortex of Rats Against Experimental Therapy of Model Insult with Derivative 3,2'- Spiro-Pyrrhol-2-Oxindole (Compound R-86) and Citicoline]. Ukrainskyi medychnyi almanakh, 16(5), 3–7. [in Ukrainian].

Mamchur, V. I., Zhuravel, N. V., Zhyliuk, V. I., & Kravchenko, K.O. (2007) Analiz vplyvu zasobiv iz tserebroprotektyvnymy vlastyvostiamy na mnestychni protsesy ta povedinkovi reaktsii shchuriv v umovakh hostroi ishemii holovnoho mozku [Analisys of cerebroprotective agents’ action on mnestic processes and behavioral reactions of rats in conditions of acute cerebral ischemia] Medychni perspektyvy, 12(1), 4–8.

Mack, J., Squier, W., & Eastman, J. T. (2009). Anatomy and development of the meninges: implications for subdural collections and CSF circulation. Pediatr. Radiol., 39, 200–210. doi: 10.1007/s00247-008-1084-6.

Cherno, B. S. & Khylko, Yu. K. (2010). Histotopohrafiia arterialnykh sudyn tverdoi obolonky holovnoho mozku ta yii pokhidnykh – stinok synusiv [Histotopography of hard arachnoid membrane’s arterial vessels and its derivatives– walls of sinuses]. Visnyk problem biolohii i medytsyny, 4, 185–188. [in Ukrainian].

Friedrich, B., Müller, F., Feiler, S., Schöller, K., & Plesnila, N. (2012). Experimental subarachnoid hemorrhage causes early and long-lasting microarterial constriction and microthrombosis: an in-vivo microscopy study. Journal of Cerebral Blood Flow & Metabolism, 32, 447–455. doi: 10.1038/jcbfm.2011.154.

Prunel dos Santos, G. F. (2003). Pathophysiology of experimental subarachnoid hemorrhage at the rat. Karolinska University Press.

Kozhemiakin, Yu. M., Khromov, O. S., Filonenko, M. A., & Saifetdinova, H. A. (2002). Naukovo-praktychni rekomendatsii z utrymannia laboratornykh tvaryn ta roboty z nymy [Scientific and practical advice on keeping laboratory animals and work with them]. Kyiv. [in Ukrainian].

Dudhani, R. V., Kyle, M., Dedeo, C., Riordan, M., & Deshaies, E. M. (2013). A Low Mortality Rat Model to Assess Delayed Cerebral Vasospasm After Experimental Subarachnoid Hemorrhage. J. Vis. Exp. 71, 4157. http://doi.org/10.3791/4157.

Aparicio, S. R., & Marsden, P. (1969). A rapid methylene blue-basic fuchsin stain for semi-thin sections of peripheral nerve and other tissues. Journal of Microscopy, 89, 139–141. doi: 10.1111/j.1365-2818.1969.tb00659.x.

Nikonenko, A. G. (2013). Vvedeniye v kolichestvennuyu gistologiyu [Introduction to quantitive histology]. Кyiv: Knyha-Plus. [in Ukrainian].

Kuvenov, A. O., Kuvenova, O. M., & Tatarenko, D. M. (patentee) (2014). Patent Ukrainy №89743 MPK G01N 1/28. Sposib vyznachennia shariv tverdoi obolonky holovnoho mozku ta yikhnikh parametriv [Patent of Ukraine №89743 MPK G01N 1/28. Method of determining of layers of brain dura mater and their parameters]. Biuleten, 8. [in Ukrainian].

Vovenko, E. P., & Chuikin, A. E. (2011). Prodol’nye gradienty napryageniya kisloroda na melchaishykh micrososudakh kory golovnogo mozga krysy pri razvitii ostroj anemii [Longitudinal oxygen gradiets in cerebral micro vessels in acute anaemia in rats]. Rossijskij fiziologicheskij zhurnal, 97(11), 1270–1280. [in Russian].

How to Cite

1.
Netlyukh AM. Pathomorphology of rat brain meninges in experimental subarachnoid hemorrhage. Pathologia [Internet]. 2015Dec.18 [cited 2024Apr.21];(3). Available from: http://pat.zsmu.edu.ua/article/view/56110

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Section

Original research