Phosphohistone H3 as the standard of immunohistochemical study in the assessment of differentiation level of oropharyngeal zone squamous cell carcinomas

Authors

  • А. V. Lysak Central Pathoanatomical Laboratory of the Ministry of Defense of Ukraine,
  • О. О. Dyadyk National Medical Academy of Postgraduate Education,

DOI:

https://doi.org/10.14739/2310-1237.2016.2.80994

Keywords:

Oropharyngeal Neoplasms, Carcinoma, Ki-67

Abstract

Verification and grading of tumors of oropharyngeal areas, determination of their malignancy potential remain an urgent problem as well as diagnosis and prognosis today.

The aim of our research was to determine the proliferative index (Ki67), and the actual number of mitoses (pHH3) and to compare them with the morphological (histological) picture.

Materials and methods. 121 cases of flat-cell cancer using monoclonal and polyclonal antibodies were analyzed in this article. The dependence between proliferation index (Ki67), the number of mitoses (pHH3) and morphological criteria was investigated. The assessment of differentiation level with Anneroth et al. 1987 u. Bryne et al. 1992 technique was used.

Results and discussion. We received the following distribution by three main groups after re-assessment of the level of differentiation. Group №1 which contains G1 tumors includes 22 patients, group №2 G2 of 45 patients and №3 G3 of 54 patients.

All three groups were morphologically described.

The groups were evaluated on the results of IHC markers Ki67 and pHH3 further.

Statistically the significant difference between groups (p <0,001 by Kruskal-Wallis test for all indicators) was found when comparing indicators Ki-67, pHH3. So with the decrease of Me level the differentiation of KI67 significantly (p <0.05) increases and the increase of pHH3 reflects the growing of tumors malignancy degree.

Conclusions. The relationship between Ki67, pHH3 and the level of differentiation of squamous cell carcinomas of oropharyngeal area has been statistically proved.

The opportunity to use pHH3 marker as a reliable criterion for assessing the level of differentiation of tumors, including squamous cell carcinomas which are difficult to be diagnosed, was set as a result of our work.

References

Gerdes, J., Stein, H., Mason, D. Y., & Ziegler, A. (1983) Human dendritic reticulum cells of lymphoid follicles: Their antigenic profile and their identification as multinucleated giant cells. Virchows Archiv B Cell Pathology Including Molecular Pathology, 42(1), 161–172. doi: 10.1007/BF02890379.

Gerdes, J., Lemke, H., Baisch, H., Wacker, H. H., Schwab, U., & Stein, H. (1984) Cell cycle analysis of a cell proliferation-associated human nuclear antigen defined by the monoclonal antibody Ki-67. The Journal of Immunology, 133(4), 1710–1715.

Van Dierendonck, J., Keijzer, R., van de Velde, C. J., & Cornelisse, C. J. (1989) Nuclear Distrsbution of the Ki-67 Antigen during the Cell Cycle: Comparsion with Growth Fraction in Human Brest Cancer Cells. Cancer Research, 49(11), 2999–3006.

Scholzen, T., & Gerdes, J. (2000) The Ki-67 Protein: From the Known and the Unknown. Journal of cellular physiology, 182(1), 311–322. doi: 10.1002/(SICI)1097-4652(200003)182:3<311::AID-JCP1>3.0.CO;2-9.

Du Manoir, S., Guillaud, P., Camus, E., Seigneurin, D., & Brugal, G. (1991) Ki-67 Labeling in Postmitotic Cell Defines Different Ki-67 Pathways Within the 2c Compartment. Cytometry, 12(1), 455–463.

Lopez, F., Belloc, F., Lacombe, F., Dumain, P., Reiffers, J., Bernard, P. & Boisseau, M. R. (1991) Modalities of Synthesis of Ki-67 Antigen During the Stimulation of Lymphocytes. Cytometry, 12(1), 42–49. doi: 10.1002/cyto.990120107.

Georg, G. Y. (2011) Phosphohistone H3: new standard ICH marker for rading tumors via mitotic indexing. MLO Med Lab Obs., 43(7), 20–23.

Retrived from: https://de.wikipedia.org/wiki/Zellzyklus.

Nowak, M., Swensson, M. A., Clarsson, J., Vogel, W., Kebschull, M., Wernert, N., et al. (2014) Prognostic significance of phospho-histone H3 in prostate carcinoma. World J. Urol., 32(1), 703–707. doi: 10.1007/s00345-013-1135-y.

Nasr, M. R., & El-Zammar, O. (2008) Comparison of pHH3, Ki-67, and survivin immunoreactivity in benign and malignant melanocytic lesions. Am J. Dermatopathol., 30(2), 117–122. doi: 10.1097/DAD.0b013e3181624054.

Skaland, I., Janssen, E., Gudlaugsson, E., Klos, J., Kjellevold, K. H., & Søiland, H., & Baak, J. P. (2007) Phosphohistone H3 expression has much stronger prognostic value than classical prognosticators in invasive lymph node-negative breast cancer patients less then 55 years of age. Modern Pathology, 20, 1307–1315. doi: 10.1038/modpathol.380097.

Uguen, A., Cong, G., Doucet, L., Talagas, M., Costa, S., De Braekeleer, M., & Marcorelles, P. (2015) Immunostaining of phosphor-histone H3 and Ki-67 improves reproducibility of recurrence risk assessment of gastrointestinal stromal tumors. Virchows Arch., 467(1), 47–54. doi: 10.1007/s00428-015-1763-2.

Diest, P., Brugal, G., & Baak, J. (1998) Proliferation markers in tumours: interpretation and clinical value. Clinical Pathology, 51(1), 716–724. doi:10.1136/jcp.51.10.716.

Kill, I. R. (1996) Localisation of the Ki-67 antigen within the nucleolus Evidence for a fibrillarin-deficient region of the dense fibrillar component. Journal of Cell Science, 109(1), 1253–1263.

Sеifert, G. (2000) Orаlpathologie III. Mundhöhle, angrenzendes Weichteil- und Knochengewebe. 1st. Berlin (Germany): Springer-Verlag.

Petri, A., & Se´bin, К. (2003) Nagladnaya statistika v medicine [Transparent statistics in medicine]. Moscow: GE´OTAR MED. [in Russian].

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1.
Lysak АV, Dyadyk ОО. Phosphohistone H3 as the standard of immunohistochemical study in the assessment of differentiation level of oropharyngeal zone squamous cell carcinomas. Pathologia [Internet]. 2016Oct.27 [cited 2024Dec.25];13(2). Available from: http://pat.zsmu.edu.ua/article/view/80994

Issue

No. 2 (2016)

Section

Original research

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